Gordonia phage Bakery

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wizardvirus; unclassified Wizardvirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DGU2|A0A514DGU2_9CAUD Uncharacterized protein OS=Gordonia phage Bakery OX=2591205 GN=38 PE=4 SV=1
MM1 pKa = 7.62FEE3 pKa = 4.42FDD5 pKa = 3.98IDD7 pKa = 3.81TSLGLCEE14 pKa = 5.96FPGCSQWATTVTEE27 pKa = 4.42RR28 pKa = 11.84TEE30 pKa = 3.5HH31 pKa = 6.09WLADD35 pKa = 3.89PEE37 pKa = 4.74LSDD40 pKa = 5.7DD41 pKa = 3.38GWANVCTDD49 pKa = 4.37HH50 pKa = 7.52APASS54 pKa = 3.79

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DGV0|A0A514DGV0_9CAUD Uncharacterized protein OS=Gordonia phage Bakery OX=2591205 GN=47 PE=4 SV=1
MM1 pKa = 7.78HH2 pKa = 7.55DD3 pKa = 3.97HH4 pKa = 6.19ATKK7 pKa = 9.97TLSASAASTEE17 pKa = 3.98SGIPKK22 pKa = 8.89RR23 pKa = 11.84TILYY27 pKa = 9.85AIQTRR32 pKa = 11.84KK33 pKa = 9.7LRR35 pKa = 11.84AEE37 pKa = 4.15KK38 pKa = 10.64LPGAGYY44 pKa = 9.75VIRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84DD50 pKa = 3.51FEE52 pKa = 3.82RR53 pKa = 11.84WRR55 pKa = 11.84EE56 pKa = 3.86SRR58 pKa = 11.84EE59 pKa = 3.91QVASS63 pKa = 3.62

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

19319

34

1838

201.2

21.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.237 ± 0.392

0.958 ± 0.129

7.174 ± 0.298

5.689 ± 0.261

2.542 ± 0.139

8.246 ± 0.509

2.179 ± 0.161

4.245 ± 0.191

2.754 ± 0.208

7.946 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.102 ± 0.121

2.93 ± 0.142

6.331 ± 0.307

3.406 ± 0.158

7.795 ± 0.387

5.021 ± 0.169

6.812 ± 0.245

7.309 ± 0.167

2.371 ± 0.13

1.957 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski