Gordonia phage Sekhmet

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DIE7|A0A514DIE7_9CAUD Uncharacterized protein OS=Gordonia phage Sekhmet OX=2591209 GN=52 PE=4 SV=1
MM1 pKa = 6.69VTPSNVAEE9 pKa = 3.98FHH11 pKa = 7.05DD12 pKa = 4.5AGEE15 pKa = 4.6GTFWWHH21 pKa = 7.15DD22 pKa = 3.55PAADD26 pKa = 4.11EE27 pKa = 4.73YY28 pKa = 11.38LITDD32 pKa = 4.49TFTPPVDD39 pKa = 3.19GALYY43 pKa = 10.63LMPWDD48 pKa = 4.55GAWFAQWDD56 pKa = 4.45GDD58 pKa = 3.46WEE60 pKa = 4.26AAVEE64 pKa = 3.96QLNSIIEE71 pKa = 4.2RR72 pKa = 11.84RR73 pKa = 11.84QEE75 pKa = 3.84TAA77 pKa = 3.28

Molecular weight: 8.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DIG4|A0A514DIG4_9CAUD Uncharacterized protein OS=Gordonia phage Sekhmet OX=2591209 GN=58 PE=4 SV=1
MM1 pKa = 6.16TTLRR5 pKa = 11.84RR6 pKa = 11.84CTRR9 pKa = 11.84PGCEE13 pKa = 3.41KK14 pKa = 10.45RR15 pKa = 11.84PRR17 pKa = 11.84SRR19 pKa = 11.84GLCHH23 pKa = 5.62THH25 pKa = 6.78YY26 pKa = 9.14MDD28 pKa = 6.13HH29 pKa = 6.62ITRR32 pKa = 11.84QKK34 pKa = 11.18AYY36 pKa = 10.13GRR38 pKa = 11.84WEE40 pKa = 3.89SSLIDD45 pKa = 3.68AEE47 pKa = 4.29PTRR50 pKa = 11.84QHH52 pKa = 4.68VQRR55 pKa = 11.84LRR57 pKa = 11.84AAGVGTRR64 pKa = 11.84KK65 pKa = 9.59ISEE68 pKa = 4.23TAGVTRR74 pKa = 11.84SVLQSLIHH82 pKa = 5.91GKK84 pKa = 7.21TRR86 pKa = 11.84NGHH89 pKa = 6.0RR90 pKa = 11.84EE91 pKa = 4.03PPSKK95 pKa = 9.88TIAKK99 pKa = 9.77RR100 pKa = 11.84IADD103 pKa = 4.46SILAVPVPAQPLHH116 pKa = 6.35NGDD119 pKa = 3.52VPALGTRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84LQALIAHH135 pKa = 7.62GYY137 pKa = 7.86SQAEE141 pKa = 3.93INRR144 pKa = 11.84RR145 pKa = 11.84FGLAADD151 pKa = 4.22NLTEE155 pKa = 5.09LIRR158 pKa = 11.84GEE160 pKa = 4.14RR161 pKa = 11.84NYY163 pKa = 10.51VDD165 pKa = 4.02PLTEE169 pKa = 3.8RR170 pKa = 11.84RR171 pKa = 11.84VTALFRR177 pKa = 11.84EE178 pKa = 4.6LQLTPGPCTRR188 pKa = 11.84ARR190 pKa = 11.84NRR192 pKa = 11.84GKK194 pKa = 10.85KK195 pKa = 9.9NGWPLPLDD203 pKa = 3.41WDD205 pKa = 4.19EE206 pKa = 5.62DD207 pKa = 4.45HH208 pKa = 7.42IDD210 pKa = 3.91DD211 pKa = 5.31PNYY214 pKa = 9.03QAEE217 pKa = 4.18RR218 pKa = 11.84SHH220 pKa = 5.94VRR222 pKa = 11.84SIVRR226 pKa = 11.84DD227 pKa = 3.49PEE229 pKa = 4.52RR230 pKa = 11.84IEE232 pKa = 3.68WRR234 pKa = 11.84KK235 pKa = 9.64EE236 pKa = 3.33RR237 pKa = 11.84VAALTAAGYY246 pKa = 9.4SAEE249 pKa = 4.42EE250 pKa = 3.47IAAQLRR256 pKa = 11.84CTARR260 pKa = 11.84TVQRR264 pKa = 11.84DD265 pKa = 3.38RR266 pKa = 11.84SDD268 pKa = 3.43LGLVDD273 pKa = 3.71QQVASS278 pKa = 3.79

Molecular weight: 31.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15485

37

2178

196.0

21.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.882 ± 0.642

0.936 ± 0.198

6.774 ± 0.336

5.819 ± 0.266

2.383 ± 0.188

8.227 ± 0.442

2.118 ± 0.234

4.598 ± 0.22

3.171 ± 0.204

7.846 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.138 ± 0.168

3.029 ± 0.259

5.735 ± 0.267

3.797 ± 0.352

7.51 ± 0.419

5.276 ± 0.285

7.427 ± 0.296

7.11 ± 0.216

1.912 ± 0.172

2.312 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski