Bacillus phage Bastille

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Bastillevirus; Bacillus virus Bastille

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9PLP1|J9PLP1_9CAUD Uncharacterized protein OS=Bacillus phage Bastille OX=57477 PE=4 SV=1
MM1 pKa = 7.59AIKK4 pKa = 10.54RR5 pKa = 11.84EE6 pKa = 3.99LLEE9 pKa = 4.11VEE11 pKa = 4.31VTFNEE16 pKa = 4.31TEE18 pKa = 4.12IYY20 pKa = 9.94AQSAKK25 pKa = 10.72DD26 pKa = 3.61AVEE29 pKa = 3.78HH30 pKa = 6.2RR31 pKa = 11.84LYY33 pKa = 11.19NIDD36 pKa = 5.97GVLEE40 pKa = 4.33WDD42 pKa = 3.41THH44 pKa = 5.0MLTSLDD50 pKa = 3.93NDD52 pKa = 4.41DD53 pKa = 4.56DD54 pKa = 4.13TTTEE58 pKa = 4.04KK59 pKa = 10.77FLLCVVYY66 pKa = 8.81NTYY69 pKa = 10.98DD70 pKa = 3.28LSGSSLEE77 pKa = 4.58DD78 pKa = 3.55VIDD81 pKa = 3.87TEE83 pKa = 5.84LYY85 pKa = 10.33TEE87 pKa = 5.11DD88 pKa = 4.3GVIGYY93 pKa = 7.63ATQPLKK99 pKa = 10.94EE100 pKa = 4.27STLDD104 pKa = 3.19ATIALEE110 pKa = 4.33EE111 pKa = 3.94ISYY114 pKa = 10.94GSSNN118 pKa = 3.09

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9PM59|J9PM59_9CAUD Minor structural protein/ putative tail fiber OS=Bacillus phage Bastille OX=57477 PE=4 SV=1
MM1 pKa = 7.44AKK3 pKa = 9.74KK4 pKa = 10.02RR5 pKa = 11.84PKK7 pKa = 10.07IFQSEE12 pKa = 4.23ANMKK16 pKa = 10.32KK17 pKa = 10.72AMNNLGNNLVNNALDD32 pKa = 3.7EE33 pKa = 4.46TAKK36 pKa = 10.84SLARR40 pKa = 11.84NLPDD44 pKa = 3.81GMEE47 pKa = 4.8LKK49 pKa = 10.56RR50 pKa = 11.84KK51 pKa = 9.02PKK53 pKa = 10.18YY54 pKa = 10.51LEE56 pKa = 3.82LTEE59 pKa = 4.68NRR61 pKa = 11.84LNSLGVIDD69 pKa = 5.17LKK71 pKa = 11.17PYY73 pKa = 9.87FARR76 pKa = 11.84SSKK79 pKa = 10.28RR80 pKa = 11.84KK81 pKa = 8.3NRR83 pKa = 11.84KK84 pKa = 8.52GGGWYY89 pKa = 8.56LTVPIRR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84ARR99 pKa = 11.84GMSRR103 pKa = 11.84RR104 pKa = 11.84MYY106 pKa = 9.26EE107 pKa = 3.53QLRR110 pKa = 11.84AIDD113 pKa = 3.65IAPSQRR119 pKa = 11.84KK120 pKa = 6.25TVISDD125 pKa = 2.95YY126 pKa = 10.63MYY128 pKa = 11.18DD129 pKa = 3.39NRR131 pKa = 11.84KK132 pKa = 7.74TSDD135 pKa = 3.12ATMLNYY141 pKa = 9.76TPRR144 pKa = 11.84SNNITKK150 pKa = 9.76IRR152 pKa = 11.84SGTNRR157 pKa = 11.84HH158 pKa = 6.43DD159 pKa = 4.16YY160 pKa = 10.48IAFRR164 pKa = 11.84TVSDD168 pKa = 3.95KK169 pKa = 11.56SPANSWIINRR179 pKa = 11.84DD180 pKa = 3.06RR181 pKa = 11.84VTVSNTSKK189 pKa = 9.82TFVANVNRR197 pKa = 11.84LMKK200 pKa = 10.92YY201 pKa = 9.54NMKK204 pKa = 10.23NQQ206 pKa = 3.17

Molecular weight: 23.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

273

0

273

47263

32

2031

173.1

19.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.068 ± 0.174

0.851 ± 0.061

6.663 ± 0.122

8.171 ± 0.29

3.997 ± 0.088

6.223 ± 0.226

1.881 ± 0.115

6.68 ± 0.123

7.854 ± 0.214

8.277 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.882 ± 0.094

5.423 ± 0.17

2.861 ± 0.121

3.288 ± 0.141

4.435 ± 0.125

5.594 ± 0.18

6.026 ± 0.223

7.035 ± 0.152

1.183 ± 0.057

4.61 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski