Streptococcus phage phiSS12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1I9S5|M1I9S5_9CAUD Putative DNA-binding phage protein OS=Streptococcus phage phiSS12 OX=1277891 GN=SS12_0048 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 3.97GTYY5 pKa = 10.33PWFDD9 pKa = 3.48YY10 pKa = 11.19DD11 pKa = 4.91RR12 pKa = 11.84DD13 pKa = 3.88YY14 pKa = 11.5LQPEE18 pKa = 4.87EE19 pKa = 4.43PRR21 pKa = 11.84QVHH24 pKa = 7.25DD25 pKa = 3.72PDD27 pKa = 2.99EE28 pKa = 4.13WVFRR32 pKa = 11.84GGQWIYY38 pKa = 11.49VGDD41 pKa = 3.78AA42 pKa = 3.3

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1IER4|M1IER4_9CAUD Putative HNH endonuclease OS=Streptococcus phage phiSS12 OX=1277891 GN=SS12_0025 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.24KK7 pKa = 9.47KK8 pKa = 10.25YY9 pKa = 9.91RR10 pKa = 11.84PIQALEE16 pKa = 3.72QWLLAISTTIDD27 pKa = 3.39IIVNGQNRR35 pKa = 11.84LYY37 pKa = 11.14NRR39 pKa = 11.84VAEE42 pKa = 4.27LEE44 pKa = 4.16NIIEE48 pKa = 4.31RR49 pKa = 11.84NAQATNSRR57 pKa = 11.84FDD59 pKa = 3.65YY60 pKa = 10.95LEE62 pKa = 4.15KK63 pKa = 10.68KK64 pKa = 9.57VANKK68 pKa = 10.12LSKK71 pKa = 10.66KK72 pKa = 9.69SWFSRR77 pKa = 11.84KK78 pKa = 8.79

Molecular weight: 9.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11026

42

1770

196.9

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.419 ± 0.439

0.526 ± 0.118

6.457 ± 0.196

7.537 ± 0.614

3.891 ± 0.252

6.33 ± 0.511

1.342 ± 0.173

6.866 ± 0.25

7.854 ± 0.389

7.9 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.204 ± 0.286

5.414 ± 0.343

2.866 ± 0.303

4.725 ± 0.256

4.798 ± 0.36

5.995 ± 0.326

6.394 ± 0.782

6.113 ± 0.317

1.297 ± 0.15

4.072 ± 0.331
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski