Streptococcus phage phiARI0004

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141E150|A0A141E150_9CAUD Uncharacterized protein OS=Streptococcus phage phiARI0004 OX=1701811 GN=phiARI0004_40 PE=4 SV=1
MM1 pKa = 7.38FNYY4 pKa = 10.14DD5 pKa = 4.02RR6 pKa = 11.84DD7 pKa = 3.52IMQPPEE13 pKa = 3.98EE14 pKa = 4.61RR15 pKa = 11.84EE16 pKa = 4.03EE17 pKa = 4.62LDD19 pKa = 3.41PADD22 pKa = 4.33WIFSAGQWIYY32 pKa = 11.59VGDD35 pKa = 4.05CC36 pKa = 3.39

Molecular weight: 4.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141E155|A0A141E155_9CAUD Uncharacterized protein OS=Streptococcus phage phiARI0004 OX=1701811 GN=phiARI0004_45 PE=4 SV=1
MM1 pKa = 7.68QEE3 pKa = 3.76LTKK6 pKa = 10.44KK7 pKa = 10.42QKK9 pKa = 10.33LKK11 pKa = 10.68KK12 pKa = 9.98QEE14 pKa = 4.21LKK16 pKa = 10.88PKK18 pKa = 9.94IKK20 pKa = 10.14LRR22 pKa = 11.84KK23 pKa = 8.15EE24 pKa = 4.07RR25 pKa = 11.84KK26 pKa = 8.3KK27 pKa = 10.38HH28 pKa = 4.81EE29 pKa = 4.04LTTVFMADD37 pKa = 4.97LIGLKK42 pKa = 9.54NRR44 pKa = 11.84RR45 pKa = 11.84QYY47 pKa = 10.16EE48 pKa = 3.97LKK50 pKa = 10.57EE51 pKa = 3.79NGKK54 pKa = 10.24APFHH58 pKa = 7.24DD59 pKa = 4.27YY60 pKa = 10.59EE61 pKa = 4.5ISIISNYY68 pKa = 6.75FHH70 pKa = 7.37KK71 pKa = 10.84SEE73 pKa = 4.62SEE75 pKa = 4.07LFFF78 pKa = 6.27

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11147

36

2425

232.2

26.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.114 ± 0.687

0.592 ± 0.13

6.396 ± 0.366

8.262 ± 0.633

4.172 ± 0.259

6.594 ± 0.624

1.238 ± 0.155

6.504 ± 0.326

8.235 ± 0.425

8.047 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.252 ± 0.231

5.239 ± 0.279

2.629 ± 0.222

4.27 ± 0.237

4.629 ± 0.357

6.091 ± 0.457

6.127 ± 0.4

6.199 ± 0.308

1.373 ± 0.187

4.037 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski