Anaerococcus prevotii ACS-065-V-Col13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Anaerococcus; Anaerococcus prevotii

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1634 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0GVF7|F0GVF7_9FIRM Phosphomethylpyrimidine kinase OS=Anaerococcus prevotii ACS-065-V-Col13 OX=879305 GN=thiD PE=3 SV=1
MM1 pKa = 7.57PADD4 pKa = 5.82LINDD8 pKa = 4.64MIPFLGILDD17 pKa = 4.06DD18 pKa = 5.76AIVLPFLIYY27 pKa = 9.12ITSSNLYY34 pKa = 9.89NKK36 pKa = 10.12QNDD39 pKa = 3.23PRR41 pKa = 11.84FSYY44 pKa = 11.0GG45 pKa = 3.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0GV62|F0GV62_9FIRM 50S ribosomal protein L14 OS=Anaerococcus prevotii ACS-065-V-Col13 OX=879305 GN=rplN PE=3 SV=1
MM1 pKa = 7.56AKK3 pKa = 10.0KK4 pKa = 10.26SLRR7 pKa = 11.84AKK9 pKa = 7.7QQRR12 pKa = 11.84KK13 pKa = 7.12QKK15 pKa = 10.37FSTRR19 pKa = 11.84EE20 pKa = 3.49YY21 pKa = 10.91SRR23 pKa = 11.84CKK25 pKa = 10.36LCGRR29 pKa = 11.84PHH31 pKa = 6.86GYY33 pKa = 9.06LRR35 pKa = 11.84KK36 pKa = 10.07YY37 pKa = 9.32GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.99LANNGQIPGVKK57 pKa = 9.29KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1634

0

1634

511794

32

4524

313.2

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.904 ± 0.061

0.733 ± 0.02

7.053 ± 0.064

7.643 ± 0.068

4.551 ± 0.054

6.255 ± 0.054

1.264 ± 0.023

9.352 ± 0.072

8.982 ± 0.067

9.124 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.03

5.902 ± 0.045

2.664 ± 0.034

2.203 ± 0.028

3.693 ± 0.046

6.478 ± 0.05

4.734 ± 0.047

6.118 ± 0.049

0.572 ± 0.015

4.209 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski