Faeces associated gemycircularvirus 11

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykroznavirus; Gemykroznavirus rabas1; Rabbit associated gemykroznavirus 1

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|T1YTA5|T1YTA5_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 11 OX=1391026 PE=3 SV=1
MM1 pKa = 7.28LVARR5 pKa = 11.84SSVPPRR11 pKa = 11.84VMPRR15 pKa = 11.84TVNWSVALEE24 pKa = 4.08SVAFLTYY31 pKa = 10.22SQYY34 pKa = 11.4GPADD38 pKa = 3.63GVSDD42 pKa = 3.67IQASSQCFEE51 pKa = 4.33YY52 pKa = 10.33IIILVAHH59 pKa = 6.59EE60 pKa = 3.85RR61 pKa = 11.84HH62 pKa = 6.34EE63 pKa = 5.04DD64 pKa = 3.63GGHH67 pKa = 6.33HH68 pKa = 5.23YY69 pKa = 10.14HH70 pKa = 7.05VLVAWEE76 pKa = 4.33SHH78 pKa = 5.41FKK80 pKa = 11.21ANDD83 pKa = 2.96VRR85 pKa = 11.84IFDD88 pKa = 4.71VGGCHH93 pKa = 7.07PNFKK97 pKa = 10.26SVRR100 pKa = 11.84GDD102 pKa = 3.39GNINRR107 pKa = 11.84VLEE110 pKa = 4.06QLRR113 pKa = 11.84LAQFLPGYY121 pKa = 8.35CLKK124 pKa = 11.18DD125 pKa = 2.95GDD127 pKa = 4.57YY128 pKa = 11.31YY129 pKa = 11.48GDD131 pKa = 3.6PEE133 pKa = 4.49VFRR136 pKa = 11.84RR137 pKa = 11.84DD138 pKa = 3.29APKK141 pKa = 10.43RR142 pKa = 11.84SQTSIWTEE150 pKa = 3.66IVSAEE155 pKa = 4.09SAGQFLQLARR165 pKa = 11.84EE166 pKa = 4.41LAPKK170 pKa = 10.16EE171 pKa = 3.68YY172 pKa = 10.49GLNYY176 pKa = 10.01DD177 pKa = 4.39RR178 pKa = 11.84LVSYY182 pKa = 10.69CRR184 pKa = 11.84AHH186 pKa = 6.0YY187 pKa = 8.54DD188 pKa = 3.09KK189 pKa = 10.6PAEE192 pKa = 4.01YY193 pKa = 10.53VPRR196 pKa = 11.84HH197 pKa = 5.32VDD199 pKa = 3.7FVVPSEE205 pKa = 3.95LDD207 pKa = 2.9DD208 pKa = 3.72WVNVNVSNAAPEE220 pKa = 4.11QPEE223 pKa = 4.22RR224 pKa = 11.84PRR226 pKa = 11.84ALCLIGRR233 pKa = 11.84SRR235 pKa = 11.84LGKK238 pKa = 7.97TEE240 pKa = 3.82WARR243 pKa = 11.84SLGKK247 pKa = 9.77HH248 pKa = 5.98SYY250 pKa = 9.45FASLFNIDD258 pKa = 5.24DD259 pKa = 4.05FDD261 pKa = 5.38GSADD265 pKa = 3.76YY266 pKa = 11.48AVMDD270 pKa = 5.72DD271 pKa = 3.68IDD273 pKa = 4.32IKK275 pKa = 10.89FFPNYY280 pKa = 9.54KK281 pKa = 9.9GWFGAQKK288 pKa = 10.17RR289 pKa = 11.84FCITGKK295 pKa = 8.46YY296 pKa = 8.94ARR298 pKa = 11.84HH299 pKa = 5.49RR300 pKa = 11.84TVDD303 pKa = 2.85WGKK306 pKa = 10.08PMIWCTNDD314 pKa = 3.66DD315 pKa = 3.4PRR317 pKa = 11.84QAVGIDD323 pKa = 3.29QDD325 pKa = 3.81WFSEE329 pKa = 4.14NPDD332 pKa = 3.35FVIVQEE338 pKa = 4.07PFLLGSLQVALALATDD354 pKa = 4.06SPRR357 pKa = 11.84FDD359 pKa = 3.36SS360 pKa = 4.38

Molecular weight:
40.94 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|T1YTA5|T1YTA5_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 11 OX=1391026 PE=3 SV=1
MM1 pKa = 7.47PRR3 pKa = 11.84YY4 pKa = 9.69AKK6 pKa = 9.26RR7 pKa = 11.84TSKK10 pKa = 10.26KK11 pKa = 9.54RR12 pKa = 11.84PAYY15 pKa = 9.56RR16 pKa = 11.84KK17 pKa = 8.54KK18 pKa = 10.17AAYY21 pKa = 9.46RR22 pKa = 11.84GKK24 pKa = 10.41KK25 pKa = 8.02RR26 pKa = 11.84TYY28 pKa = 9.94SKK30 pKa = 9.66KK31 pKa = 9.03TSSSLAKK38 pKa = 10.01KK39 pKa = 9.2IHH41 pKa = 6.18AVADD45 pKa = 4.04TKK47 pKa = 11.23KK48 pKa = 10.45SDD50 pKa = 3.3TMACAIAAPVTGANPSGVEE69 pKa = 3.96VKK71 pKa = 10.73EE72 pKa = 4.19FMDD75 pKa = 3.96WPLDD79 pKa = 3.9SQIKK83 pKa = 9.58IYY85 pKa = 9.3MWAPLYY91 pKa = 10.65RR92 pKa = 11.84VGTDD96 pKa = 2.54KK97 pKa = 11.08KK98 pKa = 11.04GPHH101 pKa = 6.39LRR103 pKa = 11.84NASRR107 pKa = 11.84VHH109 pKa = 4.41FTGVSEE115 pKa = 4.38RR116 pKa = 11.84MNIASNSRR124 pKa = 11.84VPFRR128 pKa = 11.84HH129 pKa = 5.44RR130 pKa = 11.84RR131 pKa = 11.84LVIRR135 pKa = 11.84AAVDD139 pKa = 3.69FTSRR143 pKa = 11.84MLASKK148 pKa = 10.74ADD150 pKa = 3.53PTLVFRR156 pKa = 11.84NLDD159 pKa = 3.48QLTPTALDD167 pKa = 3.78TYY169 pKa = 11.26FLGQKK174 pKa = 9.66NLDD177 pKa = 3.15WSDD180 pKa = 4.13PIKK183 pKa = 11.09ADD185 pKa = 3.29VDD187 pKa = 3.89KK188 pKa = 11.41QQLTVLFDD196 pKa = 3.05KK197 pKa = 10.63TYY199 pKa = 10.37TYY201 pKa = 11.22NPPNDD206 pKa = 3.85FGLDD210 pKa = 3.51STRR213 pKa = 11.84TSITRR218 pKa = 11.84LTAKK222 pKa = 10.33VGLPTRR228 pKa = 11.84KK229 pKa = 10.21DD230 pKa = 3.38GVNTKK235 pKa = 8.51WSAKK239 pKa = 9.99IGTKK243 pKa = 9.9EE244 pKa = 3.96PNDD247 pKa = 3.51VVIIDD252 pKa = 4.16MFRR255 pKa = 11.84SNYY258 pKa = 7.46DD259 pKa = 2.8TRR261 pKa = 11.84TNTFPLGIEE270 pKa = 3.81SWTIGSKK277 pKa = 10.52CKK279 pKa = 10.45GYY281 pKa = 11.14LEE283 pKa = 4.49GTT285 pKa = 3.83

Molecular weight:
32.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

645

285

360

322.5

36.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.062 ± 0.005

1.395 ± 0.427

7.752 ± 0.452

3.721 ± 0.995

4.651 ± 0.703

5.891 ± 0.387

2.326 ± 0.568

4.496 ± 0.04

6.512 ± 2.256

6.977 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.705 ± 0.462

3.876 ± 0.206

5.736 ± 0.075

2.946 ± 0.734

7.287 ± 0.266

7.287 ± 0.05

5.736 ± 2.517

7.287 ± 0.814

2.171 ± 0.256

4.186 ± 0.201

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski