Folsomia candida (Springtail)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Collembola; Entomobryomorpha; Isotomoidea; Isotomidae; Proisotominae; Folsomia

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28565 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A226EJN9|A0A226EJN9_FOLCA Uncharacterized protein OS=Folsomia candida OX=158441 GN=Fcan01_07653 PE=4 SV=1
MM1 pKa = 7.16ACKK4 pKa = 10.01RR5 pKa = 11.84RR6 pKa = 11.84QEE8 pKa = 4.04PDD10 pKa = 2.73TGQKK14 pKa = 10.04RR15 pKa = 11.84SSEE18 pKa = 4.27TGFKK22 pKa = 10.48PSVVWGSGRR31 pKa = 11.84EE32 pKa = 4.1DD33 pKa = 3.11VGGGHH38 pKa = 6.84FLGPVLFFCLFLFFLGPGCGRR59 pKa = 11.84GEE61 pKa = 4.46DD62 pKa = 4.1DD63 pKa = 5.47DD64 pKa = 6.19GGGSGDD70 pKa = 4.44DD71 pKa = 4.55DD72 pKa = 4.84GGSGDD77 pKa = 4.92DD78 pKa = 5.06DD79 pKa = 4.74GGSGDD84 pKa = 4.92DD85 pKa = 5.06DD86 pKa = 4.74GGSGDD91 pKa = 4.92DD92 pKa = 5.06DD93 pKa = 4.74GGSGDD98 pKa = 4.92DD99 pKa = 5.06DD100 pKa = 4.74GGSGDD105 pKa = 4.92DD106 pKa = 5.06DD107 pKa = 4.74GGSGDD112 pKa = 4.92DD113 pKa = 5.06DD114 pKa = 4.74GGSGDD119 pKa = 4.92DD120 pKa = 5.06DD121 pKa = 4.74GGSGDD126 pKa = 4.92DD127 pKa = 5.06DD128 pKa = 4.74GGSGDD133 pKa = 4.92DD134 pKa = 5.06DD135 pKa = 4.74GGSGDD140 pKa = 4.96DD141 pKa = 4.41DD142 pKa = 4.49SGSGGDD148 pKa = 4.49DD149 pKa = 3.47DD150 pKa = 5.93DD151 pKa = 4.88GGRR154 pKa = 11.84GGSSVKK160 pKa = 8.84FTYY163 pKa = 9.73FHH165 pKa = 7.05ISSDD169 pKa = 3.67LKK171 pKa = 11.14SGIVGCGSDD180 pKa = 4.84CCINWLLGLLCCVPTVVHH198 pKa = 6.55ACCFVGEE205 pKa = 4.33EE206 pKa = 4.81DD207 pKa = 4.53IGPPKK212 pKa = 10.5QPVTNVVIVQQPGQPQFHH230 pKa = 6.31VQPQQGGQFQQQCPQAQSPPQYY252 pKa = 11.24VMPQTHH258 pKa = 4.59QQYY261 pKa = 9.83GFKK264 pKa = 10.61YY265 pKa = 10.02

Molecular weight: 26.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A226DBG9|A0A226DBG9_FOLCA V(D)J recombination activating protein 1 OS=Folsomia candida OX=158441 GN=Fcan01_22896 PE=4 SV=1
MM1 pKa = 6.92STVRR5 pKa = 11.84PNFSSPPHH13 pKa = 7.01LLIAALLSVQSNSSDD28 pKa = 3.75VIHH31 pKa = 6.79GGSEE35 pKa = 3.9LFSRR39 pKa = 11.84HH40 pKa = 5.0CTTSIGRR47 pKa = 11.84RR48 pKa = 11.84DD49 pKa = 4.15DD50 pKa = 4.06LHH52 pKa = 6.24TLPSILLHH60 pKa = 5.22QFRR63 pKa = 11.84PEE65 pKa = 3.9LVQKK69 pKa = 10.13SIPARR74 pKa = 11.84VHH76 pKa = 5.01TKK78 pKa = 10.2RR79 pKa = 11.84FVMDD83 pKa = 3.73GTVGLAGLIISIPLCNKK100 pKa = 9.17GCSTMFICSGGGGIRR115 pKa = 11.84SNVCKK120 pKa = 10.22FARR123 pKa = 11.84LKK125 pKa = 11.32NNLFSHH131 pKa = 6.68NSHH134 pKa = 5.91VSYY137 pKa = 10.9CIIIVSYY144 pKa = 9.0TFITPNGDD152 pKa = 3.24SVIIIIVVVVQLPLLLRR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84QKK173 pKa = 10.75AAALCKK179 pKa = 9.39SCKK182 pKa = 9.78YY183 pKa = 10.29GQLLRR188 pKa = 11.84TRR190 pKa = 11.84RR191 pKa = 11.84LCNVPMGPASSGRR204 pKa = 11.84QTQASGGRR212 pKa = 11.84NEE214 pKa = 4.5GRR216 pKa = 11.84DD217 pKa = 3.75GSGGGSDD224 pKa = 2.88AGARR228 pKa = 11.84GEE230 pKa = 4.0MSRR233 pKa = 11.84VKK235 pKa = 10.25AGRR238 pKa = 11.84VGWVQWIHH246 pKa = 5.83PSLRR250 pKa = 11.84RR251 pKa = 11.84RR252 pKa = 11.84VSEE255 pKa = 4.39LEE257 pKa = 3.83

Molecular weight: 27.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28565

0

28565

14149570

101

16927

495.3

55.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.711 ± 0.012

1.976 ± 0.013

5.228 ± 0.01

5.993 ± 0.018

4.619 ± 0.011

6.133 ± 0.017

2.503 ± 0.009

5.977 ± 0.013

6.129 ± 0.018

9.17 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.006

4.955 ± 0.011

5.338 ± 0.016

3.837 ± 0.012

5.071 ± 0.011

8.33 ± 0.018

6.132 ± 0.02

6.257 ± 0.012

1.287 ± 0.005

3.086 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski