Cactus mild mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8PRG8|B8PRG8_9VIRU Movement protein OS=Cactus mild mottle virus OX=229030 GN=MP PE=4 SV=1
QQ1 pKa = 7.26LTTQPITRR9 pKa = 11.84GVNMYY14 pKa = 10.3IPTAKK19 pKa = 10.54CGDD22 pKa = 3.78PSDD25 pKa = 4.39LQFFYY30 pKa = 11.37DD31 pKa = 4.53AVLPNNSTVLNDD43 pKa = 3.5FDD45 pKa = 6.09SFTLTALPMQLHH57 pKa = 6.23VGDD60 pKa = 4.54CTLDD64 pKa = 3.55LSKK67 pKa = 11.13ADD69 pKa = 3.7PNLYY73 pKa = 10.11ARR75 pKa = 11.84KK76 pKa = 7.94TGFLKK81 pKa = 10.59PVLRR85 pKa = 11.84TGTEE89 pKa = 4.12LPRR92 pKa = 11.84QPNLWEE98 pKa = 4.31NLLALIKK105 pKa = 10.72RR106 pKa = 11.84NFNAPYY112 pKa = 11.11LMGTVDD118 pKa = 3.98IDD120 pKa = 3.66RR121 pKa = 11.84MAEE124 pKa = 4.04SVVNRR129 pKa = 11.84FFEE132 pKa = 4.44VYY134 pKa = 9.42IDD136 pKa = 4.97GSIEE140 pKa = 3.98SCKK143 pKa = 10.25FRR145 pKa = 11.84LLAQNTFAEE154 pKa = 4.42WLKK157 pKa = 10.73LQNPDD162 pKa = 4.81VIGKK166 pKa = 8.76LANFDD171 pKa = 4.67YY172 pKa = 10.88IDD174 pKa = 4.7LPAIDD179 pKa = 4.77QYY181 pKa = 11.93YY182 pKa = 11.11HH183 pKa = 5.92MIKK186 pKa = 10.26RR187 pKa = 11.84KK188 pKa = 9.38PKK190 pKa = 9.61IKK192 pKa = 10.16LDD194 pKa = 3.49QSICTEE200 pKa = 4.1YY201 pKa = 10.11PALQTIVYY209 pKa = 9.09HH210 pKa = 5.82SKK212 pKa = 10.4EE213 pKa = 3.67INAIFGPIFKK223 pKa = 10.34EE224 pKa = 3.79LTRR227 pKa = 11.84VFLDD231 pKa = 3.46SVDD234 pKa = 3.38SSRR237 pKa = 11.84FLFYY241 pKa = 10.18TRR243 pKa = 11.84KK244 pKa = 9.99SADD247 pKa = 3.46DD248 pKa = 3.5VSEE251 pKa = 4.29FFSDD255 pKa = 4.32LPHH258 pKa = 6.81VSQTDD263 pKa = 3.83VYY265 pKa = 10.62EE266 pKa = 4.36LDD268 pKa = 2.8ISKK271 pKa = 10.62YY272 pKa = 10.5DD273 pKa = 3.29KK274 pKa = 11.02SQNEE278 pKa = 3.73LHH280 pKa = 6.64CAIEE284 pKa = 3.96YY285 pKa = 10.08KK286 pKa = 9.93IWEE289 pKa = 4.19RR290 pKa = 11.84LGFDD294 pKa = 3.38GFLRR298 pKa = 11.84DD299 pKa = 3.27VWAQGHH305 pKa = 6.04RR306 pKa = 11.84RR307 pKa = 11.84TVLRR311 pKa = 11.84DD312 pKa = 3.53FQAGIKK318 pKa = 9.98ALIWFQRR325 pKa = 11.84KK326 pKa = 9.56SGDD329 pKa = 3.21VTTFIGNTFINAAAIATLMPLEE351 pKa = 4.43HH352 pKa = 7.02CIKK355 pKa = 10.79AGFCGDD361 pKa = 3.96DD362 pKa = 3.44SVIYY366 pKa = 9.88LPKK369 pKa = 9.05GTPVGDD375 pKa = 3.83VQAKK379 pKa = 10.63ANLEE383 pKa = 4.18WNFEE387 pKa = 3.88AKK389 pKa = 10.2LYY391 pKa = 10.49KK392 pKa = 9.16KK393 pKa = 9.17TYY395 pKa = 10.33GYY397 pKa = 10.63FCGRR401 pKa = 11.84FIIPHH406 pKa = 4.6STGAIVYY413 pKa = 9.27PDD415 pKa = 3.79VLKK418 pKa = 11.33VIAKK422 pKa = 10.34LGAKK426 pKa = 9.33DD427 pKa = 4.07VKK429 pKa = 11.1DD430 pKa = 3.99FDD432 pKa = 3.92HH433 pKa = 8.01LEE435 pKa = 3.95EE436 pKa = 5.1LRR438 pKa = 11.84VSMCDD443 pKa = 3.01TYY445 pKa = 11.47KK446 pKa = 10.81QLGNCAYY453 pKa = 11.0VDD455 pKa = 4.03LLYY458 pKa = 11.17SAMTEE463 pKa = 4.14VYY465 pKa = 9.87PSVVDD470 pKa = 3.53PRR472 pKa = 11.84FAINTVWKK480 pKa = 10.07YY481 pKa = 9.52LTDD484 pKa = 3.19KK485 pKa = 11.04RR486 pKa = 11.84LFKK489 pKa = 10.65SCHH492 pKa = 4.77GFCRR496 pKa = 11.84RR497 pKa = 11.84GPCEE501 pKa = 3.87ALEE504 pKa = 4.36SEE506 pKa = 4.58

Molecular weight: 57.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q70P76|Q70P76_9VIRU Capsid protein OS=Cactus mild mottle virus OX=229030 GN=CP PE=3 SV=1
MM1 pKa = 7.29GFVVEE6 pKa = 5.95DD7 pKa = 3.74PAKK10 pKa = 9.64HH11 pKa = 5.75LKK13 pKa = 10.16VNEE16 pKa = 3.87FLGVSGLEE24 pKa = 3.96RR25 pKa = 11.84FKK27 pKa = 11.39SVLTQPFRR35 pKa = 11.84TVSIVNSDD43 pKa = 3.21IVRR46 pKa = 11.84VASAAPLSVPIDD58 pKa = 3.28ILSSLKK64 pKa = 10.5SSKK67 pKa = 9.07LTYY70 pKa = 9.96KK71 pKa = 10.16YY72 pKa = 10.95VYY74 pKa = 9.98VLAVLISGRR83 pKa = 11.84WHH85 pKa = 6.71ISSASPGSVLLVLYY99 pKa = 10.17DD100 pKa = 4.19RR101 pKa = 11.84RR102 pKa = 11.84LQGHH106 pKa = 6.49RR107 pKa = 11.84SCIYY111 pKa = 8.78GGCMSKK117 pKa = 10.48VSANKK122 pKa = 9.52FQVKK126 pKa = 9.79YY127 pKa = 10.75SVGHH131 pKa = 6.07SLTVNDD137 pKa = 5.4FSRR140 pKa = 11.84NPLSLCVALNGVPCDD155 pKa = 4.79DD156 pKa = 3.14GWEE159 pKa = 4.05PLSIEE164 pKa = 4.26VATLLMFTNYY174 pKa = 9.57ILEE177 pKa = 4.33EE178 pKa = 4.19SLTSNILKK186 pKa = 10.02VPPLSFDD193 pKa = 3.76NNSVCLDD200 pKa = 3.24QDD202 pKa = 3.63IIMSKK207 pKa = 10.24FNSVLSTVAVPRR219 pKa = 11.84NVLKK223 pKa = 10.03CTTNFEE229 pKa = 4.5KK230 pKa = 10.71KK231 pKa = 9.86RR232 pKa = 11.84LRR234 pKa = 11.84KK235 pKa = 9.91GKK237 pKa = 8.59WVGNKK242 pKa = 9.89SEE244 pKa = 4.43FVLKK248 pKa = 10.73GDD250 pKa = 3.96TNVDD254 pKa = 3.09PMRR257 pKa = 11.84YY258 pKa = 9.54NGGFLHH264 pKa = 6.0QRR266 pKa = 11.84EE267 pKa = 4.55AEE269 pKa = 4.44HH270 pKa = 6.68ICVPDD275 pKa = 3.61SVVGRR280 pKa = 11.84TGKK283 pKa = 8.11ITQLLNPGATSHH295 pKa = 6.8LSDD298 pKa = 4.3AASSDD303 pKa = 3.86TTCC306 pKa = 4.77

Molecular weight: 33.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2115

161

1142

528.8

59.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.139 ± 0.7

2.411 ± 0.086

6.714 ± 0.494

4.397 ± 0.264

5.296 ± 0.433

5.106 ± 0.275

2.222 ± 0.177

5.248 ± 0.296

6.099 ± 0.472

8.889 ± 0.863

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.749 ± 0.209

5.012 ± 0.267

4.255 ± 0.203

3.073 ± 0.491

5.059 ± 0.295

6.998 ± 1.143

6.761 ± 0.782

8.652 ± 0.796

1.418 ± 0.141

3.499 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski