Beihai sobemo-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KED7|A0A1L3KED7_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 12 OX=1922683 PE=4 SV=1
MM1 pKa = 7.99LEE3 pKa = 4.34DD4 pKa = 3.49VTTFTSWINNINRR17 pKa = 11.84EE18 pKa = 4.24TMRR21 pKa = 11.84MDD23 pKa = 4.68PDD25 pKa = 3.83SSPGLPWGGTNGDD38 pKa = 3.32RR39 pKa = 11.84FGYY42 pKa = 9.16VPCAGYY48 pKa = 10.55DD49 pKa = 3.63PNKK52 pKa = 10.36LDD54 pKa = 4.14EE55 pKa = 4.76LRR57 pKa = 11.84MAVLIRR63 pKa = 11.84IEE65 pKa = 4.05EE66 pKa = 4.3LKK68 pKa = 10.67VAPALDD74 pKa = 4.6PIFVFIKK81 pKa = 10.62QEE83 pKa = 3.64MHH85 pKa = 6.51KK86 pKa = 10.29KK87 pKa = 9.43SKK89 pKa = 10.42AVEE92 pKa = 4.13GRR94 pKa = 11.84WRR96 pKa = 11.84LICGVGLTDD105 pKa = 4.3QIVARR110 pKa = 11.84ILFEE114 pKa = 4.51PYY116 pKa = 10.02FDD118 pKa = 4.84FLLDD122 pKa = 3.49HH123 pKa = 6.53PLIYY127 pKa = 9.33KK128 pKa = 7.74TAIGWGLTSHH138 pKa = 6.93GSLSYY143 pKa = 10.86MNFFLGGGQLQAADD157 pKa = 3.6KK158 pKa = 10.86SAWDD162 pKa = 3.43WTVQPWVFEE171 pKa = 4.13VFKK174 pKa = 10.86EE175 pKa = 4.27IMHH178 pKa = 6.28KK179 pKa = 8.42MHH181 pKa = 7.52IGRR184 pKa = 11.84EE185 pKa = 4.3DD186 pKa = 3.16LWHH189 pKa = 6.37QLMEE193 pKa = 3.93HH194 pKa = 6.87HH195 pKa = 7.15IDD197 pKa = 4.23AICYY201 pKa = 9.8HH202 pKa = 6.6KK203 pKa = 11.12VLDD206 pKa = 3.67IVGRR210 pKa = 11.84KK211 pKa = 9.03LDD213 pKa = 4.3CPPGIMPSGWFMTIAFNSVAQLILHH238 pKa = 7.31AYY240 pKa = 10.04ADD242 pKa = 4.53DD243 pKa = 3.93NNEE246 pKa = 3.85FDD248 pKa = 3.73WPFVMGDD255 pKa = 3.43DD256 pKa = 5.06TIQKK260 pKa = 8.91PASDD264 pKa = 4.4RR265 pKa = 11.84YY266 pKa = 7.25WKK268 pKa = 10.84RR269 pKa = 11.84MTTTGAKK276 pKa = 9.77IKK278 pKa = 10.59DD279 pKa = 3.21IVTTRR284 pKa = 11.84EE285 pKa = 3.52FVGFVFKK292 pKa = 10.78DD293 pKa = 3.53RR294 pKa = 11.84SYY296 pKa = 10.95HH297 pKa = 5.51PSYY300 pKa = 9.09TVKK303 pKa = 10.8YY304 pKa = 7.82NCKK307 pKa = 9.12MNNIPPEE314 pKa = 4.14VLVEE318 pKa = 4.17TLTSYY323 pKa = 9.78QWLYY327 pKa = 11.28AFEE330 pKa = 4.34PEE332 pKa = 4.05KK333 pKa = 11.03LKK335 pKa = 10.64IIHH338 pKa = 7.21RR339 pKa = 11.84YY340 pKa = 6.0MCEE343 pKa = 3.49IGASSHH349 pKa = 5.93IVTSSEE355 pKa = 3.63MADD358 pKa = 2.88RR359 pKa = 11.84VLGLKK364 pKa = 9.74SLPVGSSWW372 pKa = 3.74

Molecular weight: 42.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KED7|A0A1L3KED7_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 12 OX=1922683 PE=4 SV=1
MM1 pKa = 7.85AAQQVDD7 pKa = 3.88TFFQQWMQTSWKK19 pKa = 10.42VLEE22 pKa = 3.86QDD24 pKa = 2.86VWSSYY29 pKa = 10.41IIYY32 pKa = 10.29LRR34 pKa = 11.84VVYY37 pKa = 10.41SSSSFLMDD45 pKa = 3.65VIANFCHH52 pKa = 6.25YY53 pKa = 10.65LGDD56 pKa = 3.87RR57 pKa = 11.84PGIMNFLGFLLAVLIYY73 pKa = 10.62LVLNLPIRR81 pKa = 11.84KK82 pKa = 8.86NLSSLKK88 pKa = 10.22NRR90 pKa = 11.84YY91 pKa = 8.72KK92 pKa = 11.0DD93 pKa = 3.3SFLGSWICFFLSGFIMITKK112 pKa = 9.48YY113 pKa = 10.89AVISLLVMILWLLLTRR129 pKa = 11.84IILTTALLRR138 pKa = 11.84TGFPRR143 pKa = 11.84AVVDD147 pKa = 4.51FVVGAYY153 pKa = 9.83AAGDD157 pKa = 3.67VIVTYY162 pKa = 10.8KK163 pKa = 10.9FITLFVLGYY172 pKa = 9.39KK173 pKa = 8.78VARR176 pKa = 11.84ASFEE180 pKa = 4.24VKK182 pKa = 10.0IQADD186 pKa = 3.84EE187 pKa = 4.14SDD189 pKa = 4.08SIKK192 pKa = 10.62IPGTTEE198 pKa = 3.17IDD200 pKa = 3.53MNSCKK205 pKa = 10.43SAVAVGTIKK214 pKa = 10.64NGKK217 pKa = 9.07FNIVGQATCINAHH230 pKa = 6.33CLGIDD235 pKa = 4.04GVADD239 pKa = 3.69TFLTATHH246 pKa = 6.49IVKK249 pKa = 10.47AFPIDD254 pKa = 4.45DD255 pKa = 4.23LYY257 pKa = 10.83IWRR260 pKa = 11.84NGVAVKK266 pKa = 10.65VGLGLQCDD274 pKa = 3.84PDD276 pKa = 4.17GLFDD280 pKa = 4.06IYY282 pKa = 10.86LLRR285 pKa = 11.84VRR287 pKa = 11.84DD288 pKa = 3.9GASKK292 pKa = 10.83LGVSPAKK299 pKa = 10.18VANFPLGKK307 pKa = 10.07CAGTLFSISTEE318 pKa = 3.89GLLQSVGMIKK328 pKa = 10.02IATDD332 pKa = 3.54QEE334 pKa = 4.13DD335 pKa = 3.53SNYY338 pKa = 9.77TFIGNTIEE346 pKa = 4.16GWSGSGLYY354 pKa = 10.53VSGALVGMHH363 pKa = 5.87TVRR366 pKa = 11.84LGTDD370 pKa = 2.73KK371 pKa = 10.99KK372 pKa = 11.21LNAGYY377 pKa = 10.26DD378 pKa = 3.76ISWVPHH384 pKa = 5.74LVRR387 pKa = 11.84STLPLQHH394 pKa = 6.89RR395 pKa = 11.84CKK397 pKa = 10.21QCNEE401 pKa = 4.42EE402 pKa = 3.79IHH404 pKa = 6.85HH405 pKa = 6.43FDD407 pKa = 2.95ISEE410 pKa = 4.28GFDD413 pKa = 3.04IPKK416 pKa = 10.09KK417 pKa = 9.35QGKK420 pKa = 9.25KK421 pKa = 10.54GEE423 pKa = 4.5EE424 pKa = 3.75ICLSCDD430 pKa = 3.2NEE432 pKa = 4.4NEE434 pKa = 3.68HH435 pKa = 7.0SYY437 pKa = 11.0PFEE440 pKa = 4.48KK441 pKa = 10.15KK442 pKa = 10.24GKK444 pKa = 8.32NKK446 pKa = 10.45SSISDD451 pKa = 2.89TWDD454 pKa = 3.18YY455 pKa = 11.72LDD457 pKa = 4.66RR458 pKa = 11.84QLKK461 pKa = 9.48QDD463 pKa = 3.35STKK466 pKa = 10.65VKK468 pKa = 7.66KK469 pKa = 8.98TKK471 pKa = 9.31WGLEE475 pKa = 3.61NLYY478 pKa = 10.71AITLEE483 pKa = 3.89KK484 pKa = 10.61GKK486 pKa = 10.63VYY488 pKa = 10.8RR489 pKa = 11.84FTDD492 pKa = 4.06DD493 pKa = 4.51EE494 pKa = 5.95LDD496 pKa = 3.68TQLGAEE502 pKa = 4.07RR503 pKa = 11.84AKK505 pKa = 10.69VIRR508 pKa = 11.84ARR510 pKa = 11.84AEE512 pKa = 4.52GYY514 pKa = 10.13SSNSRR519 pKa = 11.84SDD521 pKa = 3.4YY522 pKa = 10.41FGEE525 pKa = 4.46SKK527 pKa = 11.12FEE529 pKa = 4.28GKK531 pKa = 10.58GPAQIDD537 pKa = 3.76SKK539 pKa = 10.63RR540 pKa = 11.84RR541 pKa = 11.84PIAPVDD547 pKa = 3.67LHH549 pKa = 6.59HH550 pKa = 7.02LQLARR555 pKa = 11.84LWIQNIEE562 pKa = 4.1NTQSQVSGLIRR573 pKa = 11.84ALSQLMITEE582 pKa = 4.04RR583 pKa = 11.84RR584 pKa = 11.84NWEE587 pKa = 3.74ASEE590 pKa = 4.39SEE592 pKa = 3.86EE593 pKa = 4.92DD594 pKa = 3.2MGKK597 pKa = 9.99IIQTQVDD604 pKa = 3.55IRR606 pKa = 11.84SLQLFLEE613 pKa = 4.67QIAEE617 pKa = 4.35YY618 pKa = 10.52NFEE621 pKa = 4.07ATEE624 pKa = 4.64LYY626 pKa = 10.35QLVEE630 pKa = 3.95IGSNVDD636 pKa = 2.68ITLKK640 pKa = 10.72NRR642 pKa = 11.84VEE644 pKa = 4.12RR645 pKa = 11.84QKK647 pKa = 11.2KK648 pKa = 9.98SYY650 pKa = 10.5LKK652 pKa = 9.83EE653 pKa = 4.17HH654 pKa = 7.08PLPTTSPDD662 pKa = 2.95IDD664 pKa = 3.87LTIDD668 pKa = 3.29NADD671 pKa = 3.9RR672 pKa = 11.84NLTEE676 pKa = 4.04GLEE679 pKa = 4.04ILRR682 pKa = 11.84NIPKK686 pKa = 10.36RR687 pKa = 11.84LTPEE691 pKa = 3.69DD692 pKa = 3.73TASVEE697 pKa = 4.15AAFGPVIAEE706 pKa = 3.63ILKK709 pKa = 10.36RR710 pKa = 11.84RR711 pKa = 11.84GIVAQLPMDD720 pKa = 3.95WEE722 pKa = 4.72NNDD725 pKa = 3.23GMDD728 pKa = 5.06PEE730 pKa = 5.63FDD732 pKa = 5.4DD733 pKa = 6.33DD734 pKa = 6.57DD735 pKa = 4.63EE736 pKa = 4.83EE737 pKa = 6.0QDD739 pKa = 3.8FQDD742 pKa = 4.13PRR744 pKa = 11.84STEE747 pKa = 3.54QPAPSKK753 pKa = 10.71GKK755 pKa = 10.06KK756 pKa = 9.06AALGQTTKK764 pKa = 10.97GKK766 pKa = 5.99TTSAKK771 pKa = 10.59SPATSTKK778 pKa = 10.35QSMSQEE784 pKa = 3.88RR785 pKa = 11.84KK786 pKa = 8.92PRR788 pKa = 11.84SLSRR792 pKa = 11.84GNSQRR797 pKa = 11.84TQSMSNLRR805 pKa = 11.84KK806 pKa = 9.9LEE808 pKa = 3.91KK809 pKa = 10.29EE810 pKa = 4.12KK811 pKa = 10.66QLKK814 pKa = 8.54AQYY817 pKa = 10.49EE818 pKa = 4.64SILSYY823 pKa = 7.84TQKK826 pKa = 10.4QKK828 pKa = 11.32KK829 pKa = 8.29EE830 pKa = 3.99LEE832 pKa = 4.15SLQMEE837 pKa = 4.57ISDD840 pKa = 4.21GLLKK844 pKa = 10.62EE845 pKa = 4.07SSAYY849 pKa = 9.68FRR851 pKa = 11.84KK852 pKa = 9.37QQTKK856 pKa = 9.91

Molecular weight: 96.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1228

372

856

614.0

69.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.945 ± 0.295

1.384 ± 0.119

6.352 ± 0.192

5.945 ± 0.295

4.316 ± 0.411

6.596 ± 0.065

2.036 ± 0.758

7.166 ± 0.048

7.085 ± 0.608

9.446 ± 0.718

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.881

3.664 ± 0.228

3.909 ± 0.763

4.397 ± 1.028

4.642 ± 0.177

7.41 ± 0.917

5.782 ± 0.211

5.863 ± 0.306

2.117 ± 0.716

3.339 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski