Deinococcus sp. K2S05-167

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus; unclassified Deinococcus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A418V806|A0A418V806_9DEIO Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Deinococcus sp. K2S05-167 OX=2320857 GN=D3875_12345 PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 10.62LNPKK6 pKa = 9.83LLALMTALAAGAASAAPPAPNTTSTGAGVTISNTATATFTDD47 pKa = 4.26PATNAAATPVQSNTVDD63 pKa = 3.61TVVLPQPGFDD73 pKa = 2.96IVYY76 pKa = 10.75ADD78 pKa = 3.77GTNDD82 pKa = 3.55GNTLGNTTKK91 pKa = 10.18TVTNATPGQSNSTDD105 pKa = 3.33YY106 pKa = 11.49YY107 pKa = 10.93VVNNGNTPLTVQVTANTSGSASGATVTYY135 pKa = 10.83LDD137 pKa = 3.81ASGNALPSSTTGGVTTYY154 pKa = 10.32TVTLPAGAAGMVKK167 pKa = 8.51ITQVITLPGNAPANTTYY184 pKa = 10.74GASPEE189 pKa = 4.22GTVGGTGTGTGQNGIPTGSTLYY211 pKa = 10.58EE212 pKa = 4.19NQTVTTTNGTTTVNGTPAQGADD234 pKa = 3.5LQFVKK239 pKa = 9.83LTTFKK244 pKa = 10.41PSLSATPNDD253 pKa = 4.21PNTPNPTAPDD263 pKa = 3.65GTTPATPPTLGTVQVPTVPAGTANTPNPTNPAQGYY298 pKa = 9.93LSTPSNPGDD307 pKa = 3.69PTSGGTPIVPDD318 pKa = 3.56LTGNTQIAYY327 pKa = 8.61PKK329 pKa = 10.25ADD331 pKa = 4.1PDD333 pKa = 4.1NNPTTIPAAGQTNDD347 pKa = 3.45QPGSADD353 pKa = 3.2IINFTNEE360 pKa = 3.51VKK362 pKa = 10.84NAGTVSDD369 pKa = 4.55KK370 pKa = 10.97VQLYY374 pKa = 8.68PAGPDD379 pKa = 3.27GKK381 pKa = 10.41LLAGTTFDD389 pKa = 4.04ASTGTFTLPGGIQVVFLGPNTNAAIPVASGATYY422 pKa = 7.62PTLTVPAGGSAVFRR436 pKa = 11.84TQVITTDD443 pKa = 3.48ANDD446 pKa = 3.72NLLTNAINIVVGADD460 pKa = 3.4SLNDD464 pKa = 3.46ADD466 pKa = 5.19IIADD470 pKa = 3.61ATTTDD475 pKa = 3.73VILPAAAQFGDD486 pKa = 4.1SNGTSPLGAVPTPAPVQQVTPSSNTSPSTDD516 pKa = 3.46PNLSSDD522 pKa = 3.66NVAVFPMDD530 pKa = 3.47VANLGQYY537 pKa = 8.27TDD539 pKa = 4.44SYY541 pKa = 9.77TLSATVAGLPAGATISYY558 pKa = 9.57VNSSGVALPTNGAGKK573 pKa = 10.05FVTPVVAAGQEE584 pKa = 3.94IVVYY588 pKa = 10.45AVVTVPTGTAAGNYY602 pKa = 6.83TVKK605 pKa = 10.36QQAVGNYY612 pKa = 7.15STITMTDD619 pKa = 3.25LNDD622 pKa = 3.72VIKK625 pKa = 10.75VGAVGDD631 pKa = 3.63VALAKK636 pKa = 10.17FVQDD640 pKa = 3.84GKK642 pKa = 9.6TSAGSNSYY650 pKa = 11.1AGINNPQNYY659 pKa = 7.5TVNNTSALPGTNIVYY674 pKa = 9.85QIIGKK679 pKa = 9.14NNYY682 pKa = 8.8NSPVANFALNDD693 pKa = 3.87SVPPNTTFQGAVLTINGTAVSRR715 pKa = 11.84VIYY718 pKa = 10.28KK719 pKa = 10.34VGAGTWSTTAPTVGTPAGTSIAVAADD745 pKa = 3.61TDD747 pKa = 3.95SDD749 pKa = 4.6NIPDD753 pKa = 4.46ALPAGSSMEE762 pKa = 3.99LVFTVKK768 pKa = 10.4VNN770 pKa = 3.28

Molecular weight: 76.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A418V7P3|A0A418V7P3_9DEIO Uncharacterized protein OS=Deinococcus sp. K2S05-167 OX=2320857 GN=D3875_11680 PE=4 SV=1
MM1 pKa = 7.25NRR3 pKa = 11.84NFTPEE8 pKa = 3.85LSSVYY13 pKa = 9.74RR14 pKa = 11.84DD15 pKa = 3.35EE16 pKa = 4.56GRR18 pKa = 11.84QISVALRR25 pKa = 11.84PGNYY29 pKa = 8.48TFLVMARR36 pKa = 11.84DD37 pKa = 3.6ARR39 pKa = 11.84TGRR42 pKa = 11.84TLWKK46 pKa = 10.13FHH48 pKa = 5.65GQGTASVDD56 pKa = 3.05ARR58 pKa = 11.84LAGQGAVMVTVITTGAITRR77 pKa = 11.84SQALLLDD84 pKa = 3.72ARR86 pKa = 11.84TGSVRR91 pKa = 11.84RR92 pKa = 11.84KK93 pKa = 10.48GLFTLEE99 pKa = 3.99GVRR102 pKa = 11.84DD103 pKa = 4.18GKK105 pKa = 11.03ALFMQYY111 pKa = 11.08DD112 pKa = 3.93EE113 pKa = 5.56APPSAAFLADD123 pKa = 3.85PNVLLGEE130 pKa = 4.28VMQVRR135 pKa = 11.84TGRR138 pKa = 11.84TLTRR142 pKa = 11.84NLPIPTRR149 pKa = 11.84PGCGPLKK156 pKa = 9.06TLKK159 pKa = 10.63VGSNMQAFRR168 pKa = 11.84MNDD171 pKa = 3.29RR172 pKa = 11.84QQLVATRR179 pKa = 11.84QDD181 pKa = 2.96RR182 pKa = 11.84CGTFQATFDD191 pKa = 3.33WWKK194 pKa = 10.76VPLPAPKK201 pKa = 9.53IEE203 pKa = 4.38SSASS207 pKa = 3.19

Molecular weight: 22.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4078

0

4078

1205993

28

3540

295.7

32.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.435 ± 0.043

0.632 ± 0.012

5.034 ± 0.028

5.738 ± 0.038

3.281 ± 0.023

8.525 ± 0.043

2.326 ± 0.021

3.753 ± 0.029

3.217 ± 0.034

11.516 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.019

2.785 ± 0.024

5.662 ± 0.032

4.454 ± 0.031

7.029 ± 0.039

5.157 ± 0.025

6.033 ± 0.036

7.56 ± 0.034

1.417 ± 0.017

2.386 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski