Paenibacillus sp. 598K

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6816 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401HXW9|A0A401HXW9_9BACL Sensor histidine kinase OS=Paenibacillus sp. 598K OX=1117987 GN=PA598K_02108 PE=4 SV=1
MM1 pKa = 7.31MAQDD5 pKa = 3.84NLQGNGIVYY14 pKa = 7.59TVRR17 pKa = 11.84TQHH20 pKa = 6.5KK21 pKa = 9.27DD22 pKa = 3.53YY23 pKa = 10.68PHH25 pKa = 6.78EE26 pKa = 4.79EE27 pKa = 4.03INASLIRR34 pKa = 11.84DD35 pKa = 4.33IIQDD39 pKa = 4.04FLTDD43 pKa = 3.48GDD45 pKa = 4.34DD46 pKa = 4.0DD47 pKa = 4.95FFVLEE52 pKa = 4.78PDD54 pKa = 3.45QPIQGSIYY62 pKa = 8.4MQAIGDD68 pKa = 3.99PEE70 pKa = 4.47GSGGILSEE78 pKa = 4.07VRR80 pKa = 11.84IVQDD84 pKa = 3.5DD85 pKa = 3.9EE86 pKa = 5.52SFQHH90 pKa = 6.06YY91 pKa = 10.08SYY93 pKa = 11.4LSADD97 pKa = 3.47PAEE100 pKa = 4.36AVSIFLAYY108 pKa = 9.49WEE110 pKa = 4.44TQQLPDD116 pKa = 3.7LSGWTDD122 pKa = 2.81ITAEE126 pKa = 4.17FEE128 pKa = 4.11

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A401I5H2|A0A401I5H2_9BACL Nucleoside diphosphate kinase OS=Paenibacillus sp. 598K OX=1117987 GN=ndk PE=3 SV=1
GG1 pKa = 7.22AGGPGRR7 pKa = 11.84GGPGGGSRR15 pKa = 11.84PGSPRR20 pKa = 11.84KK21 pKa = 9.78RR22 pKa = 11.84GAAGGSAGKK31 pKa = 9.06PGKK34 pKa = 10.13SGPGKK39 pKa = 10.3GGSGGPGAGGAGAAGSTGGGKK60 pKa = 9.59RR61 pKa = 11.84KK62 pKa = 9.61KK63 pKa = 10.55GKK65 pKa = 10.64GGGSSMAAFVRR76 pKa = 11.84KK77 pKa = 9.39KK78 pKa = 10.59KK79 pKa = 10.33KK80 pKa = 10.24

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6816

0

6816

2278485

19

3243

334.3

37.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.468 ± 0.034

0.707 ± 0.009

5.313 ± 0.024

6.847 ± 0.032

3.666 ± 0.022

7.726 ± 0.032

2.104 ± 0.017

5.887 ± 0.025

3.842 ± 0.023

10.466 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.016

3.143 ± 0.022

4.37 ± 0.018

4.147 ± 0.019

6.011 ± 0.031

6.087 ± 0.024

5.494 ± 0.033

6.92 ± 0.022

1.433 ± 0.015

3.659 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski