Burkholderia virus phiE122

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Tigrvirus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4JWW3|A4JWW3_9CAUD Uncharacterized protein OS=Burkholderia virus phiE122 OX=431892 GN=BPSphiE122_0018 PE=4 SV=1
MM1 pKa = 7.8NDD3 pKa = 3.35QQQSRR8 pKa = 11.84ADD10 pKa = 3.51ALTEE14 pKa = 3.77EE15 pKa = 4.75QIRR18 pKa = 11.84AFAEE22 pKa = 3.78NAFDD26 pKa = 4.13HH27 pKa = 6.42EE28 pKa = 4.83VGAGALRR35 pKa = 11.84QNVATVGNVILAIRR49 pKa = 11.84NALNLAASANEE60 pKa = 4.05TGAEE64 pKa = 4.08GAAITDD70 pKa = 3.65HH71 pKa = 6.86DD72 pKa = 4.63AKK74 pKa = 10.7GLEE77 pKa = 4.77IIAGWLYY84 pKa = 11.23KK85 pKa = 10.68DD86 pKa = 3.98GLVEE90 pKa = 3.81PAAMLRR96 pKa = 11.84RR97 pKa = 11.84LASARR102 pKa = 11.84SPAMAAAAPADD113 pKa = 3.54EE114 pKa = 5.29HH115 pKa = 6.64STIEE119 pKa = 4.39CQAHH123 pKa = 6.14SGPDD127 pKa = 3.36CTEE130 pKa = 4.19CGGTGAWSGSADD142 pKa = 3.73EE143 pKa = 4.85RR144 pKa = 11.84AALQWAAGTLQEE156 pKa = 4.49IVSGRR161 pKa = 11.84WKK163 pKa = 10.4GAKK166 pKa = 9.84EE167 pKa = 3.82SDD169 pKa = 3.44KK170 pKa = 11.27VSIGSVTKK178 pKa = 9.9TVAQVLDD185 pKa = 3.71MADD188 pKa = 3.25AALGRR193 pKa = 11.84ASQGDD198 pKa = 3.77APAEE202 pKa = 3.93MRR204 pKa = 11.84KK205 pKa = 9.31PDD207 pKa = 4.01AYY209 pKa = 11.38VPIHH213 pKa = 6.52PRR215 pKa = 11.84NGPLWASAVTSLDD228 pKa = 2.92ADD230 pKa = 3.96RR231 pKa = 11.84PKK233 pKa = 10.62SYY235 pKa = 10.23PVQAVYY241 pKa = 10.64LGASTAALSAARR253 pKa = 11.84AAASPAADD261 pKa = 3.62EE262 pKa = 4.54AQALLTVGRR271 pKa = 11.84CMGIEE276 pKa = 3.84AVEE279 pKa = 4.32KK280 pKa = 10.72YY281 pKa = 9.91IDD283 pKa = 3.81SASLGTVIDD292 pKa = 3.84YY293 pKa = 10.47AAEE296 pKa = 3.97IRR298 pKa = 11.84KK299 pKa = 8.73LAAPQPAPADD309 pKa = 3.5APADD313 pKa = 3.76VTLIPYY319 pKa = 10.23DD320 pKa = 3.55GLTEE324 pKa = 4.05EE325 pKa = 4.83FTDD328 pKa = 4.29EE329 pKa = 4.17VARR332 pKa = 11.84LANDD336 pKa = 3.24APGIRR341 pKa = 11.84EE342 pKa = 4.06AVAGALQSCGAIIAPADD359 pKa = 3.89APAHH363 pKa = 5.83AAEE366 pKa = 5.29CPHH369 pKa = 6.95CDD371 pKa = 3.29GEE373 pKa = 4.8GVIEE377 pKa = 4.5ADD379 pKa = 4.51SGASPCACAQDD390 pKa = 3.95AQEE393 pKa = 4.3GMPTFGARR401 pKa = 11.84RR402 pKa = 11.84TQADD406 pKa = 3.57APAEE410 pKa = 4.02ARR412 pKa = 11.84VV413 pKa = 3.29

Molecular weight: 42.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4JWW6|A4JWW6_9CAUD Uncharacterized protein OS=Burkholderia virus phiE122 OX=431892 GN=BPSphiE122_0021 PE=4 SV=1
MM1 pKa = 6.97EE2 pKa = 4.27QSNEE6 pKa = 3.74RR7 pKa = 11.84GVAEE11 pKa = 3.74RR12 pKa = 11.84RR13 pKa = 11.84EE14 pKa = 4.06ALFEE18 pKa = 4.51DD19 pKa = 4.45LAEE22 pKa = 4.49LGIGAGVCVPAFVVYY37 pKa = 8.84TPPRR41 pKa = 11.84TARR44 pKa = 11.84GDD46 pKa = 3.35RR47 pKa = 11.84AAANAMKK54 pKa = 10.32EE55 pKa = 4.07LRR57 pKa = 11.84PLLAKK62 pKa = 10.36LARR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84LILRR71 pKa = 3.9

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

11296

37

988

225.9

24.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.191 ± 0.631

1.027 ± 0.167

6.294 ± 0.343

5.48 ± 0.254

3.214 ± 0.206

7.994 ± 0.489

2.125 ± 0.199

4.258 ± 0.203

3.798 ± 0.296

8.401 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.165

2.797 ± 0.194

4.869 ± 0.34

3.435 ± 0.196

8.136 ± 0.424

5.046 ± 0.243

5.675 ± 0.282

7.047 ± 0.292

1.514 ± 0.137

2.399 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski