bacterium B13(2017)

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 465 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202DCB1|A0A202DCB1_9BACT Uncharacterized protein (Fragment) OS=bacterium B13(2017) OX=1932701 GN=BVX93_01295 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 10.06ILYY5 pKa = 9.59IVILFFSTYY14 pKa = 8.89SYY16 pKa = 11.79AVIFDD21 pKa = 3.75IYY23 pKa = 10.81GIDD26 pKa = 3.38EE27 pKa = 4.53GGIGSSIIDD36 pKa = 3.45LSIEE40 pKa = 4.1NNSLQLSIDD49 pKa = 3.26NTSPINLIDD58 pKa = 3.53STLPNTPGIVGFGFNLINNDD78 pKa = 3.88EE79 pKa = 4.6IFVQDD84 pKa = 3.85WNLTAYY90 pKa = 10.08DD91 pKa = 4.07FNGEE95 pKa = 4.23TFINEE100 pKa = 4.14QIGGSSNSVLDD111 pKa = 3.41KK112 pKa = 10.67WIMSDD117 pKa = 4.5FIAGVQLDD125 pKa = 5.29YY126 pKa = 11.08IPQTDD131 pKa = 3.22KK132 pKa = 11.86SMDD135 pKa = 3.28GALYY139 pKa = 10.35NPYY142 pKa = 8.8VTLQEE147 pKa = 4.32FNNLPEE153 pKa = 4.51GNNDD157 pKa = 3.23NFFTKK162 pKa = 10.65AFFTMNFDD170 pKa = 3.44QVPIIDD176 pKa = 3.53NSSFFVRR183 pKa = 11.84MQNVGEE189 pKa = 4.58DD190 pKa = 4.1GEE192 pKa = 5.06GSLKK196 pKa = 10.64LYY198 pKa = 8.98PDD200 pKa = 4.53PVPEE204 pKa = 4.62PSTLLLLSICFPFLFGLKK222 pKa = 9.88RR223 pKa = 11.84FQKK226 pKa = 10.19II227 pKa = 3.07

Molecular weight: 25.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202DDR9|A0A202DDR9_9BACT Addiction module antidote protein HigA family OS=bacterium B13(2017) OX=1932701 GN=BVX93_00535 PE=4 SV=1
MM1 pKa = 7.26IRR3 pKa = 11.84SFHH6 pKa = 6.04CKK8 pKa = 7.59KK9 pKa = 9.42TKK11 pKa = 10.66ALFNDD16 pKa = 2.66IDD18 pKa = 3.9VAKK21 pKa = 10.33FRR23 pKa = 11.84AFEE26 pKa = 3.78RR27 pKa = 11.84FARR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 10.14LMYY35 pKa = 10.48LNRR38 pKa = 11.84ARR40 pKa = 11.84NLKK43 pKa = 9.89DD44 pKa = 4.35LRR46 pKa = 11.84IPPQNRR52 pKa = 11.84LEE54 pKa = 4.05ALKK57 pKa = 10.66GDD59 pKa = 3.71RR60 pKa = 11.84KK61 pKa = 9.65GQYY64 pKa = 9.84SIRR67 pKa = 11.84INDD70 pKa = 3.07QWRR73 pKa = 11.84ICFVWKK79 pKa = 10.65NNEE82 pKa = 3.9VYY84 pKa = 10.55NVEE87 pKa = 3.75ITDD90 pKa = 3.39YY91 pKa = 10.95HH92 pKa = 6.36

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

465

0

465

261342

25

25271

562.0

64.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.775 ± 0.183

0.637 ± 0.072

6.503 ± 0.143

8.182 ± 0.24

4.703 ± 0.112

6.176 ± 0.104

1.532 ± 0.06

8.906 ± 0.179

8.102 ± 0.278

8.763 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.954 ± 0.061

6.716 ± 0.158

2.625 ± 0.147

3.377 ± 0.095

3.857 ± 0.128

7.004 ± 0.095

5.625 ± 0.168

5.19 ± 0.066

0.954 ± 0.042

4.42 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski