Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Dehalococcoidia; Dehalococcoidales; Dehalococcoidaceae; Dehalococcoides; Dehalococcoides mccartyi

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1502 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3Z7K4|Q3Z7K4_DEHM1 Major tail protein phi13 family OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) OX=243164 GN=DET1080 PE=4 SV=1
MM1 pKa = 7.55TYY3 pKa = 11.32DD4 pKa = 3.57NVIEE8 pKa = 4.05MLEE11 pKa = 4.16EE12 pKa = 4.56AGLPLAYY19 pKa = 10.36DD20 pKa = 3.87HH21 pKa = 6.69FAEE24 pKa = 5.4GEE26 pKa = 4.4SPDD29 pKa = 3.72PPFLVFLFPGTDD41 pKa = 2.92NVFADD46 pKa = 3.36DD47 pKa = 3.77TVYY50 pKa = 11.24KK51 pKa = 10.64KK52 pKa = 10.03IDD54 pKa = 3.33QLNIEE59 pKa = 5.2LYY61 pKa = 9.7TDD63 pKa = 3.32KK64 pKa = 10.73KK65 pKa = 10.89DD66 pKa = 3.67PEE68 pKa = 4.38IEE70 pKa = 4.18TTIEE74 pKa = 4.84DD75 pKa = 3.3ILLSHH80 pKa = 6.88EE81 pKa = 4.32LPYY84 pKa = 10.79EE85 pKa = 4.17KK86 pKa = 10.66SEE88 pKa = 3.8VWIEE92 pKa = 3.98SEE94 pKa = 4.12KK95 pKa = 10.3MYY97 pKa = 10.92EE98 pKa = 4.0VLYY101 pKa = 7.25QTQMIGGG108 pKa = 3.85

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3Z7P4|Q3Z7P4_DEHM1 SpoIIIJ-associated protein Jag putative OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) OX=243164 GN=DET1039 PE=4 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.97RR4 pKa = 11.84TYY6 pKa = 8.92QPKK9 pKa = 9.8RR10 pKa = 11.84IPRR13 pKa = 11.84MRR15 pKa = 11.84VHH17 pKa = 6.83GFLSRR22 pKa = 11.84MSTRR26 pKa = 11.84GGRR29 pKa = 11.84GVLSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.9KK42 pKa = 10.78LIVVV46 pKa = 4.09

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1502

0

1502

416566

30

1834

277.3

30.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.723 ± 0.096

1.367 ± 0.032

5.083 ± 0.049

6.55 ± 0.064

3.818 ± 0.049

7.61 ± 0.063

1.813 ± 0.028

7.137 ± 0.051

5.858 ± 0.053

10.302 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.026

3.867 ± 0.043

4.358 ± 0.036

3.307 ± 0.035

5.07 ± 0.049

6.353 ± 0.05

5.21 ± 0.055

6.725 ± 0.058

1.018 ± 0.025

3.198 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski