Mycobacterium virus Rumpelstiltskin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronvirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3ME81|G3ME81_9CAUD Uncharacterized protein OS=Mycobacterium virus Rumpelstiltskin OX=1086751 GN=101 PE=4 SV=1
MM1 pKa = 7.5SEE3 pKa = 4.33VYY5 pKa = 9.76TSEE8 pKa = 3.8VFARR12 pKa = 11.84CLEE15 pKa = 4.22CDD17 pKa = 3.45DD18 pKa = 5.43PIRR21 pKa = 11.84LGDD24 pKa = 4.57EE25 pKa = 3.54ITEE28 pKa = 4.15YY29 pKa = 11.03AGEE32 pKa = 4.2LFHH35 pKa = 7.5AEE37 pKa = 4.36CVV39 pKa = 3.53

Molecular weight: 4.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3ME27|G3ME27_9CAUD Esterase OS=Mycobacterium virus Rumpelstiltskin OX=1086751 GN=47 PE=4 SV=1
MM1 pKa = 6.8TVIYY5 pKa = 10.14LGEE8 pKa = 3.99PTGPRR13 pKa = 11.84LRR15 pKa = 11.84SLSRR19 pKa = 11.84TKK21 pKa = 10.54EE22 pKa = 3.89RR23 pKa = 11.84EE24 pKa = 3.26LDD26 pKa = 3.27AWLTSALKK34 pKa = 10.51NPPKK38 pKa = 10.43RR39 pKa = 11.84RR40 pKa = 11.84DD41 pKa = 3.55QIPSDD46 pKa = 3.18LAEE49 pKa = 4.04YY50 pKa = 9.54TRR52 pKa = 11.84ALSALAKK59 pKa = 10.06RR60 pKa = 11.84RR61 pKa = 11.84GIQFHH66 pKa = 6.65FNGDD70 pKa = 3.25RR71 pKa = 11.84MRR73 pKa = 11.84VRR75 pKa = 11.84GEE77 pKa = 3.84VVLGKK82 pKa = 9.89NLPGCVGGSKK92 pKa = 10.25RR93 pKa = 11.84KK94 pKa = 8.44VTVTDD99 pKa = 3.12EE100 pKa = 4.08RR101 pKa = 11.84SAVTKK106 pKa = 10.31AGQRR110 pKa = 11.84VEE112 pKa = 4.07PWNMRR117 pKa = 11.84DD118 pKa = 2.82WRR120 pKa = 11.84EE121 pKa = 3.59

Molecular weight: 13.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

21109

36

1711

191.9

21.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.683 ± 0.38

0.985 ± 0.155

6.362 ± 0.187

5.945 ± 0.307

3.188 ± 0.12

8.646 ± 0.466

1.914 ± 0.148

4.216 ± 0.154

5.041 ± 0.347

8.489 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.217 ± 0.103

3.667 ± 0.16

5.088 ± 0.293

3.591 ± 0.187

6.4 ± 0.295

5.538 ± 0.22

5.87 ± 0.281

7.243 ± 0.298

2.084 ± 0.196

2.833 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski