Bacillus virus Andromeda

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Andromedavirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1I9M7|M1I9M7_9CAUD Uncharacterized protein OS=Bacillus virus Andromeda OX=1273739 GN=ANDROMEDA_62 PE=4 SV=1
MM1 pKa = 7.49AKK3 pKa = 9.48HH4 pKa = 6.15WEE6 pKa = 4.58TICIMEE12 pKa = 5.01DD13 pKa = 2.63NWDD16 pKa = 3.78FGTMEE21 pKa = 4.17VEE23 pKa = 4.06VLEE26 pKa = 5.34GFDD29 pKa = 3.21FTYY32 pKa = 10.83AQLIEE37 pKa = 4.87DD38 pKa = 4.74YY39 pKa = 11.14YY40 pKa = 10.86MAIGDD45 pKa = 3.71QKK47 pKa = 11.73AEE49 pKa = 4.0GEE51 pKa = 4.38FIEE54 pKa = 4.31KK55 pKa = 10.36HH56 pKa = 4.96KK57 pKa = 10.55LAYY60 pKa = 9.46IGQGHH65 pKa = 6.51FGEE68 pKa = 5.2VFGLDD73 pKa = 3.3DD74 pKa = 3.92NLAIKK79 pKa = 10.19LLHH82 pKa = 6.2NLGEE86 pKa = 4.26EE87 pKa = 4.22MPDD90 pKa = 3.75GYY92 pKa = 10.53ILSQLNHH99 pKa = 6.66SDD101 pKa = 3.25MTPNVYY107 pKa = 10.4AFCDD111 pKa = 3.53QPDD114 pKa = 3.75AGFMIVDD121 pKa = 4.64KK122 pKa = 10.95IIGSNVCDD130 pKa = 3.2IHH132 pKa = 8.73SGFVLEE138 pKa = 5.85FDD140 pKa = 5.23LEE142 pKa = 4.33KK143 pKa = 10.13QLKK146 pKa = 10.15RR147 pKa = 11.84IAQFLEE153 pKa = 3.94DD154 pKa = 3.9CKK156 pKa = 10.61RR157 pKa = 11.84AEE159 pKa = 4.09VLPSDD164 pKa = 3.86LHH166 pKa = 5.69QANVMCTYY174 pKa = 10.22EE175 pKa = 5.19GDD177 pKa = 3.8LMVVDD182 pKa = 4.87VGCFEE187 pKa = 4.52YY188 pKa = 11.11GSFDD192 pKa = 3.33PHH194 pKa = 8.43SIEE197 pKa = 5.11TKK199 pKa = 8.74WCYY202 pKa = 10.81EE203 pKa = 3.77NAKK206 pKa = 10.06WEE208 pKa = 4.29MLYY211 pKa = 10.0QAYY214 pKa = 10.01FLDD217 pKa = 5.7HH218 pKa = 7.36IINDD222 pKa = 3.67QPMKK226 pKa = 10.33HH227 pKa = 6.34IPDD230 pKa = 4.35YY231 pKa = 10.44IPRR234 pKa = 11.84VFRR237 pKa = 11.84WEE239 pKa = 4.18SEE241 pKa = 4.27VAGDD245 pKa = 3.61LDD247 pKa = 3.87SRR249 pKa = 11.84VAEE252 pKa = 4.04FLKK255 pKa = 10.69DD256 pKa = 3.93FRR258 pKa = 11.84DD259 pKa = 3.45HH260 pKa = 7.43DD261 pKa = 4.38KK262 pKa = 11.38VVLPQCQQ269 pKa = 2.73

Molecular weight: 31.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1IEK9|M1IEK9_9CAUD Uncharacterized protein OS=Bacillus virus Andromeda OX=1273739 GN=ANDROMEDA_24 PE=4 SV=1
MM1 pKa = 7.21AQVTVNFNGLDD12 pKa = 3.44ARR14 pKa = 11.84LKK16 pKa = 10.68QIAKK20 pKa = 8.69EE21 pKa = 4.15CQDD24 pKa = 2.78FRR26 pKa = 11.84LPLRR30 pKa = 11.84QSSVYY35 pKa = 10.79LEE37 pKa = 4.35GSMSRR42 pKa = 11.84RR43 pKa = 11.84FSQGGGSKK51 pKa = 10.65GKK53 pKa = 9.22WKK55 pKa = 10.46SLSPATIKK63 pKa = 10.43RR64 pKa = 11.84HH65 pKa = 4.35PHH67 pKa = 5.89RR68 pKa = 11.84SGGTPLNDD76 pKa = 3.33TGRR79 pKa = 11.84LRR81 pKa = 11.84SSVSSGAVKK90 pKa = 10.45QFTPKK95 pKa = 10.5RR96 pKa = 11.84LTYY99 pKa = 10.62SIGSNVKK106 pKa = 9.14YY107 pKa = 10.82ASVHH111 pKa = 5.19NFGSGRR117 pKa = 11.84IPRR120 pKa = 11.84RR121 pKa = 11.84EE122 pKa = 3.66FMYY125 pKa = 10.46TDD127 pKa = 3.29SKK129 pKa = 11.46DD130 pKa = 3.31EE131 pKa = 4.1RR132 pKa = 11.84EE133 pKa = 4.19INKK136 pKa = 8.83VFADD140 pKa = 4.04YY141 pKa = 10.5IRR143 pKa = 11.84RR144 pKa = 11.84IAQQ147 pKa = 3.09

Molecular weight: 16.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

14749

30

1029

189.1

21.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.211 ± 0.419

0.915 ± 0.137

6.326 ± 0.291

7.838 ± 0.411

4.509 ± 0.187

6.733 ± 0.332

2.048 ± 0.173

6.733 ± 0.201

8.095 ± 0.453

7.933 ± 0.401

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.179

4.956 ± 0.354

3.153 ± 0.146

3.675 ± 0.161

4.502 ± 0.229

6.251 ± 0.359

5.865 ± 0.335

6.428 ± 0.237

1.119 ± 0.116

3.953 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski