Sutterella sp. CAG:521

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Sutterellaceae; Sutterella; environmental samples

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1632 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7KGP2|R7KGP2_9BURK Outer membrane autotransporter barrel domain protein OS=Sutterella sp. CAG:521 OX=1262977 GN=BN692_01007 PE=4 SV=1
MM1 pKa = 7.62SSFKK5 pKa = 10.62KK6 pKa = 10.55SSFVMALMVAVGSAQAASVEE26 pKa = 4.55VYY28 pKa = 9.43GTVDD32 pKa = 2.82TGLLYY37 pKa = 10.1THH39 pKa = 7.23EE40 pKa = 4.75SSDD43 pKa = 3.67SKK45 pKa = 11.66VNNEE49 pKa = 4.47KK50 pKa = 10.39KK51 pKa = 10.11HH52 pKa = 5.72EE53 pKa = 4.28SADD56 pKa = 3.41NWGVASGTNTASVIGIRR73 pKa = 11.84GMEE76 pKa = 4.64NISDD80 pKa = 4.03DD81 pKa = 3.96LSVGFVLEE89 pKa = 4.12NSFNSDD95 pKa = 3.09DD96 pKa = 3.96GSFAEE101 pKa = 4.94EE102 pKa = 3.45NTLFDD107 pKa = 4.87KK108 pKa = 10.66EE109 pKa = 4.13AQLFVTSSFGTVSLGRR125 pKa = 11.84MGALTAGEE133 pKa = 4.2GTYY136 pKa = 10.4DD137 pKa = 2.83IFMVNGDD144 pKa = 4.17AMDD147 pKa = 4.35GGYY150 pKa = 10.81ADD152 pKa = 5.0YY153 pKa = 10.86IGAGYY158 pKa = 10.13WMDD161 pKa = 3.4RR162 pKa = 11.84GIYY165 pKa = 10.37DD166 pKa = 4.04NMISFQSPEE175 pKa = 3.92FAGITAFAQYY185 pKa = 11.25SFGTSGDD192 pKa = 3.59DD193 pKa = 3.33MVPSRR198 pKa = 11.84DD199 pKa = 3.4KK200 pKa = 10.94EE201 pKa = 3.93RR202 pKa = 11.84YY203 pKa = 8.04AALGATFSAGNFSAVAVVDD222 pKa = 3.62TVMKK226 pKa = 10.55NRR228 pKa = 11.84QTVTGYY234 pKa = 11.16DD235 pKa = 3.42KK236 pKa = 11.36AIDD239 pKa = 3.62DD240 pKa = 4.52AMAFSIGANYY250 pKa = 10.95DD251 pKa = 3.29MGFMKK256 pKa = 10.32PFIGFQYY263 pKa = 9.8GQHH266 pKa = 5.27EE267 pKa = 4.28QMIGGVPVVEE277 pKa = 4.17TDD279 pKa = 3.11EE280 pKa = 4.45TEE282 pKa = 4.51SYY284 pKa = 11.26VGDD287 pKa = 3.73LDD289 pKa = 4.28GYY291 pKa = 10.46AVAVGSAFPIWGGEE305 pKa = 4.08LQVSVYY311 pKa = 10.62YY312 pKa = 11.05ADD314 pKa = 4.58GDD316 pKa = 4.06GKK318 pKa = 10.94AYY320 pKa = 10.17GYY322 pKa = 10.72DD323 pKa = 3.43QDD325 pKa = 4.99ATTQSPKK332 pKa = 9.15VSKK335 pKa = 10.53MNVKK339 pKa = 10.25RR340 pKa = 11.84YY341 pKa = 9.45GVALFHH347 pKa = 6.92NYY349 pKa = 9.05EE350 pKa = 3.95LSKK353 pKa = 10.37RR354 pKa = 11.84TSLYY358 pKa = 10.83AGIGYY363 pKa = 9.38DD364 pKa = 3.41YY365 pKa = 11.3QEE367 pKa = 4.24YY368 pKa = 10.8DD369 pKa = 3.09EE370 pKa = 6.01SYY372 pKa = 10.49VNSSEE377 pKa = 4.61KK378 pKa = 10.33EE379 pKa = 3.82HH380 pKa = 7.19SEE382 pKa = 3.8QDD384 pKa = 3.59SVQVGVGLVHH394 pKa = 6.97NFF396 pKa = 3.08

Molecular weight: 42.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7KM13|R7KM13_9BURK Alginate O-acetylation protein OS=Sutterella sp. CAG:521 OX=1262977 GN=BN692_01596 PE=3 SV=1
MM1 pKa = 7.58ALVKK5 pKa = 10.36LKK7 pKa = 8.84PTSAGRR13 pKa = 11.84RR14 pKa = 11.84HH15 pKa = 5.4AVKK18 pKa = 10.3VVSPEE23 pKa = 3.82LHH25 pKa = 6.13KK26 pKa = 11.05GKK28 pKa = 9.55PFAALLEE35 pKa = 4.4KK36 pKa = 10.46KK37 pKa = 10.45NRR39 pKa = 11.84IAGRR43 pKa = 11.84NNNGHH48 pKa = 4.98ITCRR52 pKa = 11.84HH53 pKa = 5.84KK54 pKa = 11.32GGGHH58 pKa = 5.61KK59 pKa = 9.77KK60 pKa = 9.89AYY62 pKa = 10.11RR63 pKa = 11.84IIDD66 pKa = 3.77FKK68 pKa = 11.1RR69 pKa = 11.84NKK71 pKa = 10.05DD72 pKa = 3.47GVPARR77 pKa = 11.84VEE79 pKa = 3.82RR80 pKa = 11.84LEE82 pKa = 3.89YY83 pKa = 10.73DD84 pKa = 3.78PNRR87 pKa = 11.84SAHH90 pKa = 6.08IALVLYY96 pKa = 10.73ADD98 pKa = 3.86GEE100 pKa = 4.19RR101 pKa = 11.84RR102 pKa = 11.84YY103 pKa = 10.3IIAPKK108 pKa = 9.91GLTVGAEE115 pKa = 3.93LMNGQEE121 pKa = 4.29APIKK125 pKa = 10.44VGNALPLRR133 pKa = 11.84NIPIGSTIHH142 pKa = 6.51CIEE145 pKa = 4.15MKK147 pKa = 9.67PGKK150 pKa = 9.77GAQLVRR156 pKa = 11.84SAGSFAQLIARR167 pKa = 11.84EE168 pKa = 4.17GEE170 pKa = 4.19YY171 pKa = 10.86AQVRR175 pKa = 11.84LRR177 pKa = 11.84SGEE180 pKa = 3.57VRR182 pKa = 11.84YY183 pKa = 10.05IHH185 pKa = 5.66VEE187 pKa = 3.84CRR189 pKa = 11.84ATIGMVGNEE198 pKa = 3.98EE199 pKa = 3.78NSLRR203 pKa = 11.84EE204 pKa = 4.01LGKK207 pKa = 10.63AGAKK211 pKa = 8.19RR212 pKa = 11.84WRR214 pKa = 11.84GIRR217 pKa = 11.84PTVRR221 pKa = 11.84GVAMNPVDD229 pKa = 3.85HH230 pKa = 6.82PHH232 pKa = 6.7GGGEE236 pKa = 4.05GRR238 pKa = 11.84TGTGRR243 pKa = 11.84APVTPWGQLTKK254 pKa = 10.73GYY256 pKa = 7.72RR257 pKa = 11.84TRR259 pKa = 11.84NNKK262 pKa = 8.36RR263 pKa = 11.84TDD265 pKa = 2.86AMIVQRR271 pKa = 11.84RR272 pKa = 11.84NRR274 pKa = 11.84KK275 pKa = 8.66

Molecular weight: 30.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1632

0

1632

526924

32

1847

322.9

35.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.282 ± 0.061

1.261 ± 0.025

5.345 ± 0.049

6.322 ± 0.067

4.298 ± 0.045

7.113 ± 0.066

2.048 ± 0.028

6.27 ± 0.046

5.61 ± 0.055

10.038 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.779 ± 0.034

3.707 ± 0.044

4.048 ± 0.035

3.698 ± 0.039

5.334 ± 0.055

5.959 ± 0.049

5.449 ± 0.04

7.423 ± 0.05

1.222 ± 0.022

2.793 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski