Ralstonia phage RS138

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3VI86|A0A0M3VI86_9CAUD Uncharacterized protein OS=Ralstonia phage RS138 OX=1483485 PE=4 SV=1
MM1 pKa = 7.28SQTIDD6 pKa = 3.51LQVNDD11 pKa = 4.32TALSALFDD19 pKa = 4.38RR20 pKa = 11.84YY21 pKa = 9.13QDD23 pKa = 3.11RR24 pKa = 11.84LTNAEE29 pKa = 3.85PAMAEE34 pKa = 3.54IAEE37 pKa = 4.05ILMRR41 pKa = 11.84HH42 pKa = 5.37TSDD45 pKa = 3.31AFAAEE50 pKa = 5.81GIPKK54 pKa = 9.47WVDD57 pKa = 2.98LAEE60 pKa = 4.19STKK63 pKa = 10.64AQRR66 pKa = 11.84EE67 pKa = 4.23EE68 pKa = 4.01AGTWPGMILHH78 pKa = 5.55VTGTLADD85 pKa = 4.02SYY87 pKa = 10.22TASSGNDD94 pKa = 3.33YY95 pKa = 11.26AQVASFLPYY104 pKa = 10.38AAIHH108 pKa = 5.88NFGGKK113 pKa = 9.85AGRR116 pKa = 11.84GHH118 pKa = 6.78SATTPARR125 pKa = 11.84RR126 pKa = 11.84QIPVDD131 pKa = 4.41DD132 pKa = 4.65DD133 pKa = 3.67QQLIPAARR141 pKa = 11.84EE142 pKa = 4.08DD143 pKa = 3.46ILAFMVDD150 pKa = 3.89YY151 pKa = 11.08LSAGG155 pKa = 3.39

Molecular weight: 16.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M5TK05|A0A0M5TK05_9CAUD Phage DNA transposition protein OS=Ralstonia phage RS138 OX=1483485 PE=4 SV=1
MM1 pKa = 7.48TKK3 pKa = 8.68NTNTGKK9 pKa = 9.66PAVGPIGGVAHH20 pKa = 7.63IATLDD25 pKa = 3.76LVSATLEE32 pKa = 3.92RR33 pKa = 11.84LDD35 pKa = 3.81ARR37 pKa = 11.84RR38 pKa = 11.84NGLPGIAVLYY48 pKa = 9.97GAAGWGKK55 pKa = 7.65TFAANALGQRR65 pKa = 11.84NARR68 pKa = 11.84LLRR71 pKa = 11.84ADD73 pKa = 3.7AQRR76 pKa = 11.84VALQEE81 pKa = 4.17SAGKK85 pKa = 10.05KK86 pKa = 8.97SCRR89 pKa = 11.84KK90 pKa = 7.74WASATSAPRR99 pKa = 11.84CRR101 pKa = 11.84NCWTWWLSSS110 pKa = 3.39

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12954

44

1565

235.5

25.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.003 ± 1.139

0.741 ± 0.158

5.682 ± 0.199

5.08 ± 0.493

2.617 ± 0.202

8.021 ± 0.445

1.891 ± 0.271

3.844 ± 0.201

3.559 ± 0.288

9.055 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.183

2.926 ± 0.238

4.701 ± 0.55

5.056 ± 0.4

7.48 ± 0.483

5.234 ± 0.334

6.631 ± 0.342

7.172 ± 0.449

1.528 ± 0.192

2.54 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski