Melittangium boletus DSM 14713

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Cystobacterineae; Archangiaceae; Melittangium; Melittangium boletus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7973 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A250ITA1|A0A250ITA1_9DELT GTPase OS=Melittangium boletus DSM 14713 OX=1294270 GN=MEBOL_007998 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 5.94DD3 pKa = 5.24KK4 pKa = 10.96ITLKK8 pKa = 10.31EE9 pKa = 4.11AYY11 pKa = 9.58AAMYY15 pKa = 10.14AYY17 pKa = 10.77LQMLYY22 pKa = 11.15DD23 pKa = 3.74MTGSDD28 pKa = 4.65DD29 pKa = 4.0LGGFLGSMSLLEE41 pKa = 5.57DD42 pKa = 3.91GTPADD47 pKa = 4.13PGVWDD52 pKa = 4.27DD53 pKa = 3.73WMCAVQQARR62 pKa = 11.84HH63 pKa = 5.26GQVDD67 pKa = 4.31LTLGRR72 pKa = 11.84PDD74 pKa = 3.28EE75 pKa = 4.33

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A250IEB6|A0A250IEB6_9DELT Uncharacterized protein OS=Melittangium boletus DSM 14713 OX=1294270 GN=MEBOL_002931 PE=4 SV=1
MM1 pKa = 7.85PPRR4 pKa = 11.84GSSSTRR10 pKa = 11.84SKK12 pKa = 10.8SSKK15 pKa = 8.41AQKK18 pKa = 9.85SVRR21 pKa = 11.84TRR23 pKa = 11.84AKK25 pKa = 9.96RR26 pKa = 11.84AVTRR30 pKa = 11.84ARR32 pKa = 11.84KK33 pKa = 7.35TVAKK37 pKa = 10.25AAGKK41 pKa = 8.23TKK43 pKa = 9.77TAVKK47 pKa = 10.15RR48 pKa = 11.84AAQDD52 pKa = 3.18KK53 pKa = 9.46RR54 pKa = 11.84ASPARR59 pKa = 11.84RR60 pKa = 11.84TRR62 pKa = 11.84STGIKK67 pKa = 9.76ARR69 pKa = 11.84RR70 pKa = 11.84ATSASGKK77 pKa = 7.6TGRR80 pKa = 11.84KK81 pKa = 7.75VSRR84 pKa = 11.84SPRR87 pKa = 11.84AKK89 pKa = 9.95KK90 pKa = 10.36VPRR93 pKa = 11.84TSTPIAAII101 pKa = 3.82

Molecular weight: 10.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7973

0

7973

2961835

29

8113

371.5

40.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.355 ± 0.033

0.954 ± 0.012

4.965 ± 0.02

6.622 ± 0.03

3.387 ± 0.015

8.661 ± 0.03

2.186 ± 0.011

3.055 ± 0.019

2.941 ± 0.023

11.206 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.012

2.254 ± 0.019

6.105 ± 0.023

3.426 ± 0.017

8.029 ± 0.029

5.962 ± 0.024

5.401 ± 0.025

7.892 ± 0.025

1.449 ± 0.011

2.18 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski