Cellulomonas sp. NEAU-YY56

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4017 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M2J461|A0A3M2J461_9CELL Alpha-D-glucose phosphate-specific phosphoglucomutase OS=Cellulomonas sp. NEAU-YY56 OX=2483352 GN=EBM89_12640 PE=3 SV=1
MM1 pKa = 7.01TAVPHH6 pKa = 5.37GRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84GIVTGAGLVATALVLAACGGSGDD33 pKa = 4.49DD34 pKa = 4.32SSDD37 pKa = 3.22GGGDD41 pKa = 3.51GGSGGALLIGTTDD54 pKa = 4.02KK55 pKa = 10.49ITTIDD60 pKa = 3.49PAGSYY65 pKa = 11.17DD66 pKa = 3.54NGSFAVMNQIYY77 pKa = 8.82PFLMNTPYY85 pKa = 10.85GSPDD89 pKa = 3.56VEE91 pKa = 3.89PDD93 pKa = 3.01IAEE96 pKa = 4.25SAEE99 pKa = 4.09FTSPTEE105 pKa = 3.79YY106 pKa = 10.53TVTLKK111 pKa = 10.98EE112 pKa = 4.14GLTFANGNDD121 pKa = 3.63LTSSDD126 pKa = 3.75VKK128 pKa = 10.88FSFDD132 pKa = 2.97RR133 pKa = 11.84QLAIFEE139 pKa = 4.56SGADD143 pKa = 3.78DD144 pKa = 4.27GNGPGSLLYY153 pKa = 11.01NLDD156 pKa = 3.53SVAAPDD162 pKa = 4.09DD163 pKa = 3.73LTVVFTLKK171 pKa = 10.97SPDD174 pKa = 3.65DD175 pKa = 3.57QVFPQILSSPVGPIVDD191 pKa = 3.83EE192 pKa = 4.73DD193 pKa = 4.16VFAADD198 pKa = 4.7ALTPDD203 pKa = 3.73AEE205 pKa = 4.49IVDD208 pKa = 4.35GQAFAGPYY216 pKa = 10.22VLTDD220 pKa = 3.23YY221 pKa = 10.96AQNDD225 pKa = 4.17LLSYY229 pKa = 8.41TANPDD234 pKa = 3.47YY235 pKa = 10.82QGLLGAPKK243 pKa = 9.69TEE245 pKa = 4.66EE246 pKa = 4.11INVQYY251 pKa = 9.51YY252 pKa = 10.09TDD254 pKa = 3.67ASNLKK259 pKa = 10.73LEE261 pKa = 4.43VQQGTVDD268 pKa = 3.28VAFRR272 pKa = 11.84SLSATDD278 pKa = 3.76IEE280 pKa = 5.27DD281 pKa = 4.16LRR283 pKa = 11.84GDD285 pKa = 4.08DD286 pKa = 3.5NVKK289 pKa = 10.33VVDD292 pKa = 4.44GPGGEE297 pKa = 3.65IRR299 pKa = 11.84YY300 pKa = 8.28ITFNFNTQPYY310 pKa = 9.66GATTPEE316 pKa = 4.22ADD318 pKa = 3.52PAKK321 pKa = 10.57ALAVRR326 pKa = 11.84QAVADD331 pKa = 4.63LIDD334 pKa = 3.98RR335 pKa = 11.84EE336 pKa = 4.31EE337 pKa = 4.55IADD340 pKa = 3.64QVYY343 pKa = 10.41KK344 pKa = 10.16GTYY347 pKa = 7.53TPLYY351 pKa = 9.34SYY353 pKa = 10.97VPEE356 pKa = 4.62GLTGANEE363 pKa = 4.08ALKK366 pKa = 10.81GLYY369 pKa = 10.38GDD371 pKa = 4.56GEE373 pKa = 4.58GGADD377 pKa = 3.34ADD379 pKa = 4.19KK380 pKa = 11.05AAEE383 pKa = 4.1TLEE386 pKa = 4.26AAGVEE391 pKa = 4.6TPVQLSLQYY400 pKa = 11.43SNDD403 pKa = 3.17HH404 pKa = 6.36YY405 pKa = 11.18GPSSGEE411 pKa = 3.74EE412 pKa = 3.59YY413 pKa = 11.33ALIKK417 pKa = 10.67DD418 pKa = 3.81QLEE421 pKa = 4.46STGLFAVDD429 pKa = 4.19LQTTEE434 pKa = 3.56WVQYY438 pKa = 11.09AKK440 pKa = 10.96DD441 pKa = 3.58RR442 pKa = 11.84TADD445 pKa = 3.41VYY447 pKa = 10.63PAYY450 pKa = 10.26QLGWFPDD457 pKa = 3.67YY458 pKa = 11.48SDD460 pKa = 4.11ADD462 pKa = 3.57NYY464 pKa = 8.96LTPFFLTDD472 pKa = 3.18NFLGNHH478 pKa = 6.09YY479 pKa = 11.16DD480 pKa = 3.59NAEE483 pKa = 4.06VNDD486 pKa = 5.44LILQQAVTTDD496 pKa = 3.3TAEE499 pKa = 3.89RR500 pKa = 11.84TALIGEE506 pKa = 4.25IQDD509 pKa = 4.82AVAQDD514 pKa = 4.57LSTVPYY520 pKa = 10.3LQGAQVAVVGADD532 pKa = 3.2VTGAEE537 pKa = 4.4DD538 pKa = 3.51TLDD541 pKa = 3.3ASFKK545 pKa = 10.38FRR547 pKa = 11.84YY548 pKa = 8.66GALAIGG554 pKa = 3.91

Molecular weight: 58.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M2JFS1|A0A3M2JFS1_9CELL DUF885 domain-containing protein OS=Cellulomonas sp. NEAU-YY56 OX=2483352 GN=EBM89_07975 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4017

0

4017

1294718

29

3745

322.3

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.894 ± 0.064

0.527 ± 0.01

6.587 ± 0.04

5.04 ± 0.035

2.422 ± 0.023

9.61 ± 0.04

2.035 ± 0.023

2.683 ± 0.028

1.204 ± 0.02

10.459 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.509 ± 0.016

1.369 ± 0.018

6.048 ± 0.037

2.626 ± 0.022

7.91 ± 0.052

4.739 ± 0.03

6.54 ± 0.046

10.486 ± 0.048

1.529 ± 0.016

1.783 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski