Agromyces ramosus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7MK88|A0A4Q7MK88_9MICO Uncharacterized protein DUF1713 OS=Agromyces ramosus OX=33879 GN=EV187_1108 PE=4 SV=1
MM1 pKa = 7.32PQAEE5 pKa = 4.55LPRR8 pKa = 11.84YY9 pKa = 8.11ILAMTRR15 pKa = 11.84ISGALLAGILLAALTGCVPAADD37 pKa = 4.14AAPPTSEE44 pKa = 4.59PPSPTASAEE53 pKa = 4.02PEE55 pKa = 4.12PTVMAKK61 pKa = 8.14PTLAFAGACEE71 pKa = 4.09ALVDD75 pKa = 4.7DD76 pKa = 4.97ATLSDD81 pKa = 4.57LVGDD85 pKa = 4.35DD86 pKa = 3.25MAIAVEE92 pKa = 3.94KK93 pKa = 10.56SEE95 pKa = 4.58RR96 pKa = 11.84VWAVGVLGGLDD107 pKa = 4.71CIWLSDD113 pKa = 3.65SEE115 pKa = 5.51AYY117 pKa = 10.55VSLDD121 pKa = 3.31VLPAAGLEE129 pKa = 4.29AQVSRR134 pKa = 11.84AGADD138 pKa = 3.0SPYY141 pKa = 10.15CYY143 pKa = 10.09GGEE146 pKa = 4.03PSEE149 pKa = 4.45SRR151 pKa = 11.84CSFSTVIDD159 pKa = 4.72GYY161 pKa = 8.94WLSGIVGVANGSANTALDD179 pKa = 4.69AIDD182 pKa = 5.67AITEE186 pKa = 4.11RR187 pKa = 11.84VADD190 pKa = 4.14AASAAAPVSAARR202 pKa = 11.84PDD204 pKa = 4.07GTWSVPAEE212 pKa = 4.26CSTLADD218 pKa = 3.76AVDD221 pKa = 3.63TTGILGATFAAEE233 pKa = 3.99QEE235 pKa = 4.75SVGGEE240 pKa = 4.09VTSGFIAALHH250 pKa = 5.77AVGDD254 pKa = 4.77LSCTWATPDD263 pKa = 3.28YY264 pKa = 10.64ARR266 pKa = 11.84WFTSEE271 pKa = 4.53LLPGAGWAISEE282 pKa = 4.12LAARR286 pKa = 11.84AGAAPVTVDD295 pKa = 3.4GALQAVALPLGDD307 pKa = 3.84GQTTAVYY314 pKa = 8.56ATDD317 pKa = 3.93GVNLAWVTVPLEE329 pKa = 3.96IDD331 pKa = 3.18QTQAATLTSAVMAAASRR348 pKa = 3.73

Molecular weight: 35.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7MK98|A0A4Q7MK98_9MICO NADH dehydrogenase OS=Agromyces ramosus OX=33879 GN=EV187_0725 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3588

0

3588

1163547

27

2016

324.3

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.069 ± 0.062

0.458 ± 0.007

6.392 ± 0.033

6.02 ± 0.039

3.138 ± 0.027

9.126 ± 0.036

2.009 ± 0.018

4.295 ± 0.028

1.608 ± 0.027

10.081 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.71 ± 0.013

1.772 ± 0.019

5.616 ± 0.034

2.457 ± 0.022

7.669 ± 0.05

5.307 ± 0.026

5.667 ± 0.028

9.134 ± 0.031

1.548 ± 0.018

1.925 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski