Dragonfly-associated microphage 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0A1M0|K0A1M0_9VIRU Uncharacterized protein OS=Dragonfly-associated microphage 1 OX=1234888 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.02FDD4 pKa = 3.55EE5 pKa = 5.17KK6 pKa = 10.95GRR8 pKa = 11.84EE9 pKa = 4.2LPDD12 pKa = 3.44PTPIEE17 pKa = 4.09IPTGFRR23 pKa = 11.84RR24 pKa = 11.84PEE26 pKa = 3.83TLQEE30 pKa = 3.88QIRR33 pKa = 11.84RR34 pKa = 11.84LIRR37 pKa = 11.84SEE39 pKa = 3.82QFAAQMGRR47 pKa = 11.84PDD49 pKa = 3.48AEE51 pKa = 4.38SFEE54 pKa = 4.35EE55 pKa = 5.0ANDD58 pKa = 3.7FDD60 pKa = 5.08VDD62 pKa = 4.41DD63 pKa = 5.6DD64 pKa = 4.84GEE66 pKa = 4.55LPVTQHH72 pKa = 6.12EE73 pKa = 4.71FTAMALEE80 pKa = 4.5KK81 pKa = 10.88PNDD84 pKa = 3.86EE85 pKa = 5.09YY86 pKa = 11.8DD87 pKa = 4.27DD88 pKa = 5.93ADD90 pKa = 4.1DD91 pKa = 3.71NPRR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84EE97 pKa = 3.9VAAGPAGDD105 pKa = 4.14RR106 pKa = 11.84APSRR110 pKa = 11.84DD111 pKa = 3.83DD112 pKa = 3.69SDD114 pKa = 4.14HH115 pKa = 7.08GSDD118 pKa = 5.2ADD120 pKa = 4.13PDD122 pKa = 4.15DD123 pKa = 3.95EE124 pKa = 6.16AGSVRR129 pKa = 11.84RR130 pKa = 11.84DD131 pKa = 3.24PGQRR135 pKa = 11.84GPAGSRR141 pKa = 11.84SVGDD145 pKa = 3.48VKK147 pKa = 10.74EE148 pKa = 4.32SRR150 pKa = 11.84QPAQGDD156 pKa = 3.3SGRR159 pKa = 11.84RR160 pKa = 11.84SKK162 pKa = 10.93GASGTT167 pKa = 3.72

Molecular weight: 18.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0A2J2|K0A2J2_9VIRU Major capsid protein OS=Dragonfly-associated microphage 1 OX=1234888 PE=3 SV=1
MM1 pKa = 7.55ARR3 pKa = 11.84RR4 pKa = 11.84APRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84ISSPVQSTFTPTSQDD24 pKa = 2.66RR25 pKa = 11.84ATPARR30 pKa = 11.84WSSRR34 pKa = 11.84LAWLPRR40 pKa = 11.84SNLTAVTKK48 pKa = 10.78ALADD52 pKa = 3.32QVFQYY57 pKa = 10.57VRR59 pKa = 11.84EE60 pKa = 4.25SYY62 pKa = 10.59RR63 pKa = 11.84PGPRR67 pKa = 11.84AMVVYY72 pKa = 10.32RR73 pKa = 11.84PSQKK77 pKa = 9.96RR78 pKa = 11.84LSRR81 pKa = 11.84RR82 pKa = 11.84SRR84 pKa = 11.84VDD86 pKa = 2.99LLRR89 pKa = 11.84PVVRR93 pKa = 11.84PNKK96 pKa = 9.99SRR98 pKa = 11.84NFCQLRR104 pKa = 11.84RR105 pKa = 11.84EE106 pKa = 4.16RR107 pKa = 11.84KK108 pKa = 8.02EE109 pKa = 3.76VMFARR114 pKa = 11.84GVAGGRR120 pKa = 11.84WGRR123 pKa = 11.84RR124 pKa = 11.84GPDD127 pKa = 3.03MYY129 pKa = 10.6RR130 pKa = 11.84ASRR133 pKa = 11.84TAYY136 pKa = 10.88SNLTCRR142 pKa = 11.84RR143 pKa = 3.64

Molecular weight: 16.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1343

143

485

268.6

30.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.084 ± 1.031

0.67 ± 0.413

6.106 ± 1.34

6.106 ± 0.799

3.723 ± 0.155

8.042 ± 0.617

1.862 ± 0.461

2.755 ± 0.351

3.202 ± 0.571

7.297 ± 0.952

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.137

2.978 ± 0.367

6.255 ± 0.532

4.84 ± 0.467

10.722 ± 1.848

6.627 ± 0.733

5.287 ± 0.676

6.404 ± 0.623

2.085 ± 0.508

3.425 ± 0.49
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski