Dokdonia sp. (strain 4H-3-7-5) (Krokinobacter sp. (strain 4H-3-7-5))

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Dokdonia; unclassified Dokdonia

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2978 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4B1M5|F4B1M5_DOKS4 Uncharacterized protein OS=Dokdonia sp. (strain 4H-3-7-5) OX=983548 GN=Krodi_0556 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.35KK3 pKa = 10.14FLFIILLCSYY13 pKa = 10.88AFAKK17 pKa = 10.58AQTTDD22 pKa = 3.1PALLVNIQKK31 pKa = 10.65ASQAEE36 pKa = 3.52IDD38 pKa = 4.0AFNIANLEE46 pKa = 3.77IGMLVYY52 pKa = 9.97NTDD55 pKa = 2.79VNRR58 pKa = 11.84IFEE61 pKa = 4.4YY62 pKa = 7.66TTSGFLVILTDD73 pKa = 3.59ADD75 pKa = 3.5VSEE78 pKa = 4.58TVTTFVDD85 pKa = 3.54NGNGTATYY93 pKa = 10.05TNEE96 pKa = 3.59NNTAVTVGIVGPQGPAGADD115 pKa = 3.3GATGATGPQGPAGADD130 pKa = 3.3GATGATGPQGPAGADD145 pKa = 3.3GATGATGPQGPAGADD160 pKa = 3.3GATGATGPQGPAGADD175 pKa = 3.3GATGATGAIGPQGPAGADD193 pKa = 3.3GATGATGPQGPIGPKK208 pKa = 9.26GTDD211 pKa = 3.19GATGATGPQGPAGADD226 pKa = 3.3GATGATGPQGDD237 pKa = 4.63TGPQGPTGATGADD250 pKa = 4.21GATGPAGPTGAQGPVGPQGPAGPTGAQGVEE280 pKa = 4.89GPQGPVGPSGTYY292 pKa = 9.04TGFFIIDD299 pKa = 3.52NSTLNTTGTYY309 pKa = 9.13SQSISGIPFEE319 pKa = 4.49PSQVTFKK326 pKa = 10.71AHH328 pKa = 6.21PNIGSFNINDD338 pKa = 3.92DD339 pKa = 3.88NALGANNTGLLEE351 pKa = 4.17NTFGAMNGFARR362 pKa = 11.84AGSPIAQAVIFSGGSGSSINNISRR386 pKa = 11.84YY387 pKa = 10.31ANNTQCIGLRR397 pKa = 11.84YY398 pKa = 8.09TNNNGSNLGVISASLASFTATGFNLNVTYY427 pKa = 10.21TLGTTGSAEE436 pKa = 4.14VQNDD440 pKa = 3.21ILDD443 pKa = 3.66EE444 pKa = 4.23SVIVLFTAYY453 pKa = 9.47EE454 pKa = 3.89

Molecular weight: 43.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4AZV2|F4AZV2_DOKS4 Transcription termination factor Rho OS=Dokdonia sp. (strain 4H-3-7-5) OX=983548 GN=rho PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.92GRR40 pKa = 11.84KK41 pKa = 8.34KK42 pKa = 10.21ISVSSEE48 pKa = 3.74TRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 10.68KK53 pKa = 9.8

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2978

0

2978

1016983

31

5218

341.5

38.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.23 ± 0.048

0.698 ± 0.013

6.061 ± 0.067

6.577 ± 0.038

4.93 ± 0.044

6.682 ± 0.044

1.75 ± 0.03

7.573 ± 0.042

6.802 ± 0.077

9.126 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.026

5.524 ± 0.062

3.453 ± 0.029

3.452 ± 0.028

3.731 ± 0.041

6.352 ± 0.038

6.346 ± 0.096

6.566 ± 0.047

0.989 ± 0.02

3.907 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski