Wenling sobemo-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFA0|A0A1L3KFA0_9VIRU RNA-directed RNA polymerase OS=Wenling sobemo-like virus 1 OX=1923540 PE=4 SV=1
MM1 pKa = 6.87STLVSIVKK9 pKa = 10.38DD10 pKa = 3.51LVDD13 pKa = 3.43VEE15 pKa = 4.91NPVDD19 pKa = 4.33YY20 pKa = 7.62PTLDD24 pKa = 3.3WFNHH28 pKa = 4.71VLLHH32 pKa = 6.88DD33 pKa = 4.07VTPSSSTGLPWKK45 pKa = 10.1KK46 pKa = 10.19RR47 pKa = 11.84GMDD50 pKa = 3.18KK51 pKa = 10.97GSSFVEE57 pKa = 4.26FGEE60 pKa = 4.04IKK62 pKa = 10.45EE63 pKa = 4.31NAIHH67 pKa = 7.18LYY69 pKa = 9.95DD70 pKa = 3.7AVVSRR75 pKa = 11.84VLALSEE81 pKa = 4.25GVEE84 pKa = 4.4SDD86 pKa = 5.01PISCFIKK93 pKa = 10.54PEE95 pKa = 3.72PHH97 pKa = 6.66KK98 pKa = 10.29PSKK101 pKa = 10.94VKK103 pKa = 10.76DD104 pKa = 3.6GAWRR108 pKa = 11.84IISGVGITDD117 pKa = 3.85CFVDD121 pKa = 5.05RR122 pKa = 11.84MLFGNFLNGSIEE134 pKa = 4.09KK135 pKa = 10.59ARR137 pKa = 11.84TLDD140 pKa = 3.6NPLLPGFVPYY150 pKa = 10.65FGGYY154 pKa = 9.0RR155 pKa = 11.84DD156 pKa = 3.65LARR159 pKa = 11.84KK160 pKa = 8.68FVRR163 pKa = 11.84PEE165 pKa = 3.79TADD168 pKa = 3.25KK169 pKa = 11.24SSWDD173 pKa = 3.27WTVQGWMVTFLRR185 pKa = 11.84RR186 pKa = 11.84FFEE189 pKa = 4.46AVTDD193 pKa = 4.31SKK195 pKa = 10.85WHH197 pKa = 6.43GILNNRR203 pKa = 11.84LTALFQDD210 pKa = 3.96AEE212 pKa = 4.49LDD214 pKa = 3.69FEE216 pKa = 5.1GYY218 pKa = 10.24RR219 pKa = 11.84FRR221 pKa = 11.84QNCDD225 pKa = 3.09GIMKK229 pKa = 9.86SGCLGTIIFNSLLQLAIHH247 pKa = 6.18VLSCMRR253 pKa = 11.84SGEE256 pKa = 4.08DD257 pKa = 3.45LKK259 pKa = 11.14PFYY262 pKa = 10.9AVGDD266 pKa = 3.76DD267 pKa = 4.73TIQEE271 pKa = 4.42TCSEE275 pKa = 4.46EE276 pKa = 3.9YY277 pKa = 9.16WDD279 pKa = 3.94EE280 pKa = 4.15VKK282 pKa = 10.78KK283 pKa = 11.03CGAIVKK289 pKa = 9.2EE290 pKa = 4.9RR291 pKa = 11.84IGGWPAEE298 pKa = 4.15FIGIYY303 pKa = 10.68FNDD306 pKa = 4.0TVGLPMYY313 pKa = 8.17ATKK316 pKa = 10.98NFFRR320 pKa = 11.84LLFAEE325 pKa = 5.4AEE327 pKa = 4.14LLDD330 pKa = 5.71DD331 pKa = 4.27ILAGYY336 pKa = 7.89MRR338 pKa = 11.84LYY340 pKa = 11.05ANSPLFTILNEE351 pKa = 3.62LAIRR355 pKa = 11.84KK356 pKa = 8.39GVTLSKK362 pKa = 10.64DD363 pKa = 3.42DD364 pKa = 3.88CLTWFNGG371 pKa = 3.39

Molecular weight: 41.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFA0|A0A1L3KFA0_9VIRU RNA-directed RNA polymerase OS=Wenling sobemo-like virus 1 OX=1923540 PE=4 SV=1
MM1 pKa = 7.33FGIKK5 pKa = 10.03ALAEE9 pKa = 4.01RR10 pKa = 11.84VLGGVMAVIRR20 pKa = 11.84FVYY23 pKa = 10.0VVGVFALQVAAADD36 pKa = 3.84VLLVAGDD43 pKa = 3.61YY44 pKa = 10.64TNYY47 pKa = 8.8NTVANLANGNGLYY60 pKa = 10.34NWLLPTTYY68 pKa = 10.14FKK70 pKa = 11.03HH71 pKa = 6.53LFTALVVVIAGRR83 pKa = 11.84FMFEE87 pKa = 3.88FRR89 pKa = 11.84STVNALKK96 pKa = 10.33PPSYY100 pKa = 11.0DD101 pKa = 3.19GDD103 pKa = 3.98ADD105 pKa = 3.89RR106 pKa = 11.84MKK108 pKa = 10.83AYY110 pKa = 9.2TFTVKK115 pKa = 10.51DD116 pKa = 3.98FSKK119 pKa = 11.02DD120 pKa = 3.42YY121 pKa = 9.99MRR123 pKa = 11.84QPGFTSEE130 pKa = 3.96SMRR133 pKa = 11.84AGSVIHH139 pKa = 6.4TNLGEE144 pKa = 4.43VKK146 pKa = 10.48GCLHH150 pKa = 6.41VLDD153 pKa = 5.13NNSKK157 pKa = 8.13TVGFAQVVKK166 pKa = 9.77GHH168 pKa = 6.01IVMPEE173 pKa = 4.08HH174 pKa = 7.76VLMAADD180 pKa = 4.75GGRR183 pKa = 11.84LCGPYY188 pKa = 10.34GGLVALNPKK197 pKa = 9.2MFEE200 pKa = 4.04LVKK203 pKa = 10.03TDD205 pKa = 3.49VVVCPLVEE213 pKa = 4.01SHH215 pKa = 6.99RR216 pKa = 11.84KK217 pKa = 8.47ILAVKK222 pKa = 10.23DD223 pKa = 3.21ADD225 pKa = 3.58FRR227 pKa = 11.84PSFRR231 pKa = 11.84NGAFAQMRR239 pKa = 11.84TTPLMDD245 pKa = 5.38PDD247 pKa = 3.34AHH249 pKa = 5.73MVKK252 pKa = 10.02PGCVVQGKK260 pKa = 7.44VQPNDD265 pKa = 3.18MFGMVSFSGSTRR277 pKa = 11.84PGMSGGGYY285 pKa = 9.65YY286 pKa = 10.32VDD288 pKa = 3.92GALVAMHH295 pKa = 6.93LCGGLSNMGVSMGFVMEE312 pKa = 4.41SVARR316 pKa = 11.84PEE318 pKa = 4.49ADD320 pKa = 3.15LDD322 pKa = 3.89TYY324 pKa = 10.46EE325 pKa = 4.36WLMKK329 pKa = 9.88SAEE332 pKa = 4.13YY333 pKa = 10.65EE334 pKa = 5.26DD335 pKa = 4.38GFEE338 pKa = 4.49WDD340 pKa = 3.63NTHH343 pKa = 7.3DD344 pKa = 4.03PSLVIVKK351 pKa = 9.87DD352 pKa = 3.52VNNRR356 pKa = 11.84YY357 pKa = 9.75HH358 pKa = 7.14EE359 pKa = 4.32VDD361 pKa = 3.87LDD363 pKa = 3.68YY364 pKa = 11.51APAEE368 pKa = 4.33FYY370 pKa = 10.95DD371 pKa = 3.75VLGMRR376 pKa = 11.84KK377 pKa = 9.12PKK379 pKa = 10.5VGVRR383 pKa = 11.84NRR385 pKa = 11.84GIDD388 pKa = 3.75YY389 pKa = 10.69SNKK392 pKa = 10.19DD393 pKa = 3.78YY394 pKa = 11.26EE395 pKa = 4.48PEE397 pKa = 3.94AKK399 pKa = 9.88VGYY402 pKa = 9.35DD403 pKa = 3.31HH404 pKa = 7.5QIMKK408 pKa = 9.29TGYY411 pKa = 10.26LKK413 pKa = 10.92DD414 pKa = 3.69PSSLPKK420 pKa = 10.59EE421 pKa = 4.02MLPVGTRR428 pKa = 11.84LRR430 pKa = 11.84AFVEE434 pKa = 4.28EE435 pKa = 4.72EE436 pKa = 4.04DD437 pKa = 5.94VEE439 pKa = 4.86DD440 pKa = 4.65QDD442 pKa = 6.53DD443 pKa = 3.84PVQIDD448 pKa = 3.67DD449 pKa = 3.73AFQRR453 pKa = 11.84ARR455 pKa = 11.84DD456 pKa = 3.7RR457 pKa = 11.84LHH459 pKa = 7.52DD460 pKa = 3.79YY461 pKa = 10.29FKK463 pKa = 11.56NLIAEE468 pKa = 4.7LAADD472 pKa = 3.84VARR475 pKa = 11.84PSKK478 pKa = 10.8EE479 pKa = 3.37EE480 pKa = 3.43AAINVEE486 pKa = 4.04PFLEE490 pKa = 4.21EE491 pKa = 3.47SRR493 pKa = 11.84ALVEE497 pKa = 4.12QKK499 pKa = 11.08GKK501 pKa = 10.05ISRR504 pKa = 11.84RR505 pKa = 11.84LQEE508 pKa = 4.41INTVMHH514 pKa = 6.81GAKK517 pKa = 10.01KK518 pKa = 8.96AQKK521 pKa = 9.72VGKK524 pKa = 7.55TQLLASKK531 pKa = 7.98TQTLEE536 pKa = 3.5EE537 pKa = 3.89MRR539 pKa = 11.84QAKK542 pKa = 8.6EE543 pKa = 3.99MKK545 pKa = 9.67KK546 pKa = 9.88HH547 pKa = 6.48ADD549 pKa = 3.08AATQLLRR556 pKa = 11.84TNDD559 pKa = 3.52LKK561 pKa = 11.22KK562 pKa = 10.16KK563 pKa = 10.3SKK565 pKa = 10.17NARR568 pKa = 11.84QNAKK572 pKa = 10.08RR573 pKa = 11.84KK574 pKa = 8.45EE575 pKa = 4.26QVDD578 pKa = 3.82QMRR581 pKa = 11.84LTQDD585 pKa = 3.18YY586 pKa = 11.2LMEE589 pKa = 4.05QLQQKK594 pKa = 8.23TGQTANDD601 pKa = 3.81LHH603 pKa = 7.23QQMLDD608 pKa = 3.06AMRR611 pKa = 11.84ARR613 pKa = 11.84PSTSQAGFIKK623 pKa = 10.55KK624 pKa = 9.9

Molecular weight: 69.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

995

371

624

497.5

55.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.638 ± 1.369

1.307 ± 0.518

7.236 ± 0.189

5.729 ± 0.123

5.126 ± 0.982

7.538 ± 0.17

2.111 ± 0.3

3.719 ± 1.347

6.633 ± 0.592

9.045 ± 0.729

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.819 ± 1.013

4.121 ± 0.047

4.221 ± 0.056

3.317 ± 1.199

5.226 ± 0.228

5.226 ± 0.757

4.824 ± 0.181

8.543 ± 0.935

1.307 ± 0.846

3.317 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski