Wenling crustacean virus 11

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alphacrustrhavirus; Zhejiang alphacrustrhavirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN83|A0A1L3KN83_9VIRU Uncharacterized protein OS=Wenling crustacean virus 11 OX=1923480 PE=4 SV=1
MM1 pKa = 7.51ASGVASLEE9 pKa = 3.8RR10 pKa = 11.84LIRR13 pKa = 11.84STDD16 pKa = 2.68ISLNSGRR23 pKa = 11.84GITWNDD29 pKa = 3.01QTLKK33 pKa = 10.8KK34 pKa = 9.81FWKK37 pKa = 9.45RR38 pKa = 11.84HH39 pKa = 4.22YY40 pKa = 10.4AVPNADD46 pKa = 3.86LMKK49 pKa = 10.59SIYY52 pKa = 10.21TDD54 pKa = 3.96FLSAYY59 pKa = 8.94CGKK62 pKa = 10.18FAGFQTGPLIRR73 pKa = 11.84AAILSTLTPPGDD85 pKa = 3.04KK86 pKa = 11.01HH87 pKa = 5.24MFGYY91 pKa = 9.8VFKK94 pKa = 10.54GVVPIYY100 pKa = 10.32DD101 pKa = 3.86PSAPPPPRR109 pKa = 11.84ATPTAKK115 pKa = 9.29TQVPQQSAIQTEE127 pKa = 4.49LSSANTYY134 pKa = 8.97LAKK137 pKa = 10.54DD138 pKa = 3.44NHH140 pKa = 7.02DD141 pKa = 3.51ISSDD145 pKa = 3.86DD146 pKa = 3.74EE147 pKa = 5.87SDD149 pKa = 5.07DD150 pKa = 4.17EE151 pKa = 6.98GDD153 pKa = 4.97DD154 pKa = 4.44EE155 pKa = 6.37PITPPDD161 pKa = 2.97IVGFQEE167 pKa = 4.11TMLKK171 pKa = 10.49YY172 pKa = 10.87IMIAPGNSYY181 pKa = 9.68MVLGFLAMICFRR193 pKa = 11.84IIVRR197 pKa = 11.84DD198 pKa = 3.78PKK200 pKa = 10.84AIRR203 pKa = 11.84LYY205 pKa = 10.21FANQRR210 pKa = 11.84RR211 pKa = 11.84ILALISSCTGVVMRR225 pKa = 11.84TGIPAPTAAFLDD237 pKa = 4.15DD238 pKa = 4.55LVVSIPKK245 pKa = 9.88GQTIASEE252 pKa = 4.33VLADD256 pKa = 3.55MTYY259 pKa = 11.6LLIKK263 pKa = 10.06SGPASPVVRR272 pKa = 11.84FLEE275 pKa = 4.83GGCMVHH281 pKa = 7.6LSGNGLGLIDD291 pKa = 6.29LIDD294 pKa = 3.72RR295 pKa = 11.84VANKK299 pKa = 10.68YY300 pKa = 9.54EE301 pKa = 3.91VTIAMVLSLLLTSRR315 pKa = 11.84SVKK318 pKa = 9.77SVGRR322 pKa = 11.84VVEE325 pKa = 4.31FLVNAPKK332 pKa = 10.29ILSWKK337 pKa = 7.96WSRR340 pKa = 11.84AITDD344 pKa = 3.52RR345 pKa = 11.84AFQDD349 pKa = 4.16LRR351 pKa = 11.84VQNNLTLTGYY361 pKa = 9.91VVALAAEE368 pKa = 4.51ADD370 pKa = 3.79AEE372 pKa = 4.37SEE374 pKa = 3.7IWKK377 pKa = 9.7IRR379 pKa = 11.84ALEE382 pKa = 4.26DD383 pKa = 3.39LPAATKK389 pKa = 10.32AQAIAWAKK397 pKa = 10.15QYY399 pKa = 11.16LILRR403 pKa = 11.84DD404 pKa = 3.89KK405 pKa = 8.84EE406 pKa = 4.6TKK408 pKa = 9.26TSHH411 pKa = 6.54FEE413 pKa = 3.2ASAAYY418 pKa = 9.54RR419 pKa = 11.84RR420 pKa = 11.84VAAGIEE426 pKa = 4.27SEE428 pKa = 4.33EE429 pKa = 4.35EE430 pKa = 3.82EE431 pKa = 4.74SEE433 pKa = 4.81SDD435 pKa = 4.72DD436 pKa = 4.24GDD438 pKa = 3.52ISSDD442 pKa = 3.74EE443 pKa = 5.83DD444 pKa = 3.59DD445 pKa = 5.28APWNN449 pKa = 3.68

Molecular weight: 49.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KN87|A0A1L3KN87_9VIRU Uncharacterized protein OS=Wenling crustacean virus 11 OX=1923480 PE=4 SV=1
MM1 pKa = 8.05LMGRR5 pKa = 11.84VDD7 pKa = 3.63ATVTHH12 pKa = 5.93YY13 pKa = 10.93SEE15 pKa = 4.04YY16 pKa = 7.17TTRR19 pKa = 11.84HH20 pKa = 3.99EE21 pKa = 4.34TIALVILSGLFDD33 pKa = 4.76PYY35 pKa = 11.06LPRR38 pKa = 11.84EE39 pKa = 4.18SLFVLASEE47 pKa = 4.5LSLRR51 pKa = 11.84FSLAEE56 pKa = 4.5SISPDD61 pKa = 3.23CVMGVSDD68 pKa = 4.56GKK70 pKa = 10.08PVVIHH75 pKa = 5.44LQKK78 pKa = 9.92RR79 pKa = 11.84TATVKK84 pKa = 10.45VLYY87 pKa = 10.2ALPDD91 pKa = 3.71EE92 pKa = 5.19GWTHH96 pKa = 5.21TGEE99 pKa = 4.26YY100 pKa = 9.99EE101 pKa = 4.07NTFSAEE107 pKa = 4.23TSCSFGIVSTNCSIHH122 pKa = 5.1ATVRR126 pKa = 11.84QKK128 pKa = 10.82KK129 pKa = 9.76VSDD132 pKa = 3.84VVSKK136 pKa = 11.26KK137 pKa = 10.5MIDD140 pKa = 3.01QGLGYY145 pKa = 10.16RR146 pKa = 11.84RR147 pKa = 11.84LRR149 pKa = 11.84GVGDD153 pKa = 3.35VTRR156 pKa = 11.84GLLINIHH163 pKa = 4.06QTIRR167 pKa = 11.84EE168 pKa = 4.21KK169 pKa = 10.37GTEE172 pKa = 3.69RR173 pKa = 11.84ALKK176 pKa = 9.07HH177 pKa = 5.06VKK179 pKa = 10.13RR180 pKa = 11.84SEE182 pKa = 3.86EE183 pKa = 3.67AASFVGEE190 pKa = 4.16SSLVGPAGGSKK201 pKa = 10.81ALTFLKK207 pKa = 10.9NFVGKK212 pKa = 9.98NN213 pKa = 3.15

Molecular weight: 23.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3742

213

2106

748.4

83.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.44 ± 1.216

1.336 ± 0.322

5.104 ± 0.734

6.173 ± 0.535

3.554 ± 0.277

6.467 ± 0.299

2.352 ± 0.286

5.746 ± 0.423

5.852 ± 0.85

10.689 ± 0.679

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.031 ± 0.273

2.94 ± 0.177

5.158 ± 0.543

3.394 ± 0.255

5.211 ± 0.125

9.621 ± 0.555

6.173 ± 0.384

6.601 ± 0.475

2.138 ± 0.446

3.02 ± 0.38
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski