Salmonella phage vB_SenS_Sasha

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sashavirus; Salmonella virus Sasha

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8DTM0|A0A1P8DTM0_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_Sasha OX=1913114 GN=CPTSasha_12 PE=4 SV=1
MM1 pKa = 7.78NNLLLNKK8 pKa = 9.74KK9 pKa = 8.45IVKK12 pKa = 8.29MQIASDD18 pKa = 3.78KK19 pKa = 10.29EE20 pKa = 3.82ALLFVTDD27 pKa = 5.48DD28 pKa = 3.77GQQLIVRR35 pKa = 11.84VDD37 pKa = 3.81AEE39 pKa = 4.75CCSHH43 pKa = 5.55TWIEE47 pKa = 4.49SVEE50 pKa = 4.06LPALGFPFTVIACDD64 pKa = 3.68DD65 pKa = 3.87LDD67 pKa = 4.0MNKK70 pKa = 9.92EE71 pKa = 3.79PLEE74 pKa = 4.18NEE76 pKa = 4.12EE77 pKa = 3.55YY78 pKa = 10.48DD79 pKa = 3.55YY80 pKa = 11.68LQFYY84 pKa = 8.68GAKK87 pKa = 8.33ITTDD91 pKa = 3.12KK92 pKa = 11.27GDD94 pKa = 3.55LVIDD98 pKa = 3.88YY99 pKa = 9.81RR100 pKa = 11.84NSSNGYY106 pKa = 8.08YY107 pKa = 10.16GGSIVWPGEE116 pKa = 3.43DD117 pKa = 3.17HH118 pKa = 7.44FYY120 pKa = 11.47GGVFGQNISDD130 pKa = 4.38EE131 pKa = 4.38EE132 pKa = 4.35WEE134 pKa = 4.98DD135 pKa = 3.41IQDD138 pKa = 3.72AGGGDD143 pKa = 3.28NHH145 pKa = 6.63EE146 pKa = 4.42PVV148 pKa = 3.59

Molecular weight: 16.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8DTS9|A0A1P8DTS9_9CAUD Putative lipoprotein OS=Salmonella phage vB_SenS_Sasha OX=1913114 GN=CPTSasha_72 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 9.94PRR4 pKa = 11.84YY5 pKa = 9.61RR6 pKa = 11.84LMRR9 pKa = 11.84LSQCPNGTRR18 pKa = 11.84FYY20 pKa = 10.62FYY22 pKa = 10.67RR23 pKa = 11.84LKK25 pKa = 10.69QLLPFTLIANSFKK38 pKa = 10.28KK39 pKa = 10.09HH40 pKa = 4.64EE41 pKa = 4.86SIYY44 pKa = 10.96HH45 pKa = 6.08EE46 pKa = 3.95GHH48 pKa = 7.07DD49 pKa = 4.3FNITARR55 pKa = 11.84KK56 pKa = 8.79YY57 pKa = 8.07RR58 pKa = 11.84TSIHH62 pKa = 5.97KK63 pKa = 9.51LVWAKK68 pKa = 10.96VNN70 pKa = 3.44

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

16597

46

971

204.9

22.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.616 ± 0.516

1.265 ± 0.143

6.001 ± 0.222

6.031 ± 0.295

3.543 ± 0.195

7.134 ± 0.33

1.862 ± 0.212

5.603 ± 0.209

5.634 ± 0.256

7.7 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.549 ± 0.167

5.26 ± 0.226

4.645 ± 0.315

4.296 ± 0.5

5.236 ± 0.255

5.766 ± 0.312

6.236 ± 0.331

7.188 ± 0.254

1.609 ± 0.128

3.826 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski