Hoeflea phototrophica (strain DSM 17068 / NCIMB 14078 / DFL-43)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Hoeflea; Hoeflea phototrophica

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9D7Y7|A9D7Y7_HOEPD Prephenate dehydrogenase OS=Hoeflea phototrophica (strain DSM 17068 / NCIMB 14078 / DFL-43) OX=411684 GN=HPDFL43_16911 PE=4 SV=1
MM1 pKa = 7.57NDD3 pKa = 2.63ISQGKK8 pKa = 7.75NARR11 pKa = 11.84QPATLTSNLPIDD23 pKa = 3.86QEE25 pKa = 4.55GAGPLKK31 pKa = 10.59SRR33 pKa = 11.84IVITITGGVAEE44 pKa = 5.04LADD47 pKa = 3.91AEE49 pKa = 4.27NWPSGLEE56 pKa = 3.97LYY58 pKa = 10.22IVDD61 pKa = 3.69YY62 pKa = 10.95DD63 pKa = 4.25VNEE66 pKa = 3.94GDD68 pKa = 4.12YY69 pKa = 11.52DD70 pKa = 4.11EE71 pKa = 6.19DD72 pKa = 4.23CADD75 pKa = 3.49VDD77 pKa = 4.48GEE79 pKa = 4.36CSIRR83 pKa = 11.84CYY85 pKa = 11.11DD86 pKa = 3.78SIHH89 pKa = 6.37RR90 pKa = 11.84VSNSYY95 pKa = 9.5AQNVAMEE102 pKa = 4.12YY103 pKa = 10.67EE104 pKa = 4.28RR105 pKa = 11.84LNASS109 pKa = 3.2

Molecular weight: 11.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9DH42|A9DH42_HOEPD Branched-chain-amino-acid aminotransferase OS=Hoeflea phototrophica (strain DSM 17068 / NCIMB 14078 / DFL-43) OX=411684 GN=ilvE PE=3 SV=1
MM1 pKa = 7.76SDD3 pKa = 3.72HH4 pKa = 6.47NRR6 pKa = 11.84ASQGRR11 pKa = 11.84LARR14 pKa = 11.84FCEE17 pKa = 4.13TGKK20 pKa = 10.74LCLFLKK26 pKa = 10.48RR27 pKa = 11.84LFGARR32 pKa = 11.84TVIRR36 pKa = 11.84ADD38 pKa = 3.56GLDD41 pKa = 3.34EE42 pKa = 4.25RR43 pKa = 11.84LKK45 pKa = 10.74RR46 pKa = 11.84DD47 pKa = 2.9IGIAGGIHH55 pKa = 7.49DD56 pKa = 5.12GGLDD60 pKa = 3.61GSARR64 pKa = 11.84PDD66 pKa = 3.31PRR68 pKa = 11.84GDD70 pKa = 2.85HH71 pKa = 5.56YY72 pKa = 11.4RR73 pKa = 11.84RR74 pKa = 11.84LLRR77 pKa = 11.84RR78 pKa = 11.84GYY80 pKa = 9.77PLGG83 pKa = 3.85

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4185

0

4185

1320306

20

3620

315.5

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.95 ± 0.041

0.874 ± 0.012

5.928 ± 0.031

5.912 ± 0.033

3.875 ± 0.026

8.573 ± 0.036

2.041 ± 0.016

5.628 ± 0.026

3.507 ± 0.028

9.75 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.017

2.83 ± 0.019

4.903 ± 0.029

3.092 ± 0.019

6.482 ± 0.039

5.859 ± 0.029

5.289 ± 0.031

7.237 ± 0.03

1.292 ± 0.014

2.202 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski