Bacillus phage B5S

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Bequatrovirus; Bacillus virus B4; Bacillus phage B4

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 272 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9PRI5|J9PRI5_9CAUD Uncharacterized protein OS=Bacillus phage B5S OX=1126949 GN=B5S_0215 PE=4 SV=1
MM1 pKa = 7.17YY2 pKa = 10.23SWNNEE7 pKa = 3.68DD8 pKa = 5.28FEE10 pKa = 5.07FEE12 pKa = 4.21GLVGKK17 pKa = 8.15TFTRR21 pKa = 11.84IKK23 pKa = 11.03NEE25 pKa = 3.79GSDD28 pKa = 3.41EE29 pKa = 4.13LVFYY33 pKa = 9.52DD34 pKa = 4.02TDD36 pKa = 4.01GEE38 pKa = 4.54IYY40 pKa = 11.1VMYY43 pKa = 9.82HH44 pKa = 5.33QQDD47 pKa = 3.89CCEE50 pKa = 3.9SVYY53 pKa = 10.66IEE55 pKa = 6.01DD56 pKa = 4.19IDD58 pKa = 5.85GDD60 pKa = 4.37LEE62 pKa = 4.64DD63 pKa = 5.43LLHH66 pKa = 7.07SPILMAEE73 pKa = 4.22AVSEE77 pKa = 4.26SGNDD81 pKa = 3.17SDD83 pKa = 5.59YY84 pKa = 11.06GTSTWTFYY92 pKa = 11.03KK93 pKa = 10.27IATIKK98 pKa = 10.04GTVTLRR104 pKa = 11.84WLGEE108 pKa = 4.07SNGYY112 pKa = 9.78YY113 pKa = 9.83SEE115 pKa = 4.79SVDD118 pKa = 3.9FVRR121 pKa = 11.84VTDD124 pKa = 4.05EE125 pKa = 3.8EE126 pKa = 4.49DD127 pKa = 3.1KK128 pKa = 11.1RR129 pKa = 11.84SYY131 pKa = 10.71QEE133 pKa = 3.82NNN135 pKa = 2.88

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9PQS5|J9PQS5_9CAUD Uncharacterized protein OS=Bacillus phage B5S OX=1126949 GN=B5S_0038 PE=4 SV=1
MM1 pKa = 6.84TRR3 pKa = 11.84HH4 pKa = 5.4VRR6 pKa = 11.84YY7 pKa = 9.46KK8 pKa = 10.23QLNKK12 pKa = 9.81ATIKK16 pKa = 10.38QATINSIMLRR26 pKa = 11.84TPSIEE31 pKa = 4.23EE32 pKa = 3.47NRR34 pKa = 11.84QVYY37 pKa = 9.8KK38 pKa = 11.25AMGILDD44 pKa = 4.1EE45 pKa = 4.64GFLWFSPRR53 pKa = 11.84RR54 pKa = 11.84WHH56 pKa = 5.51QVKK59 pKa = 7.8MTNLIYY65 pKa = 10.1LTGKK69 pKa = 10.29EE70 pKa = 4.47SFDD73 pKa = 3.36AMKK76 pKa = 10.81ARR78 pKa = 11.84IFQIIDD84 pKa = 2.96VRR86 pKa = 11.84TWKK89 pKa = 10.72

Molecular weight: 10.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

272

0

272

48806

43

2019

179.4

20.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.962 ± 0.19

0.809 ± 0.063

6.391 ± 0.141

8.495 ± 0.332

3.977 ± 0.106

6.485 ± 0.232

1.842 ± 0.098

6.596 ± 0.134

8.144 ± 0.198

8.104 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.85 ± 0.083

5.342 ± 0.133

2.926 ± 0.136

3.319 ± 0.136

4.268 ± 0.11

5.372 ± 0.159

6.225 ± 0.232

6.899 ± 0.17

1.217 ± 0.058

4.778 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski