Anhanga virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Anhanga phlebovirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SHW0|A0A1S5SHW0_9VIRU Nucleoprotein OS=Anhanga virus OX=904722 PE=3 SV=1
MM1 pKa = 7.45LNYY4 pKa = 10.5YY5 pKa = 10.59LFDD8 pKa = 4.27RR9 pKa = 11.84PVVKK13 pKa = 10.38KK14 pKa = 10.59AEE16 pKa = 4.31KK17 pKa = 10.18KK18 pKa = 9.48RR19 pKa = 11.84VVYY22 pKa = 10.08YY23 pKa = 9.07MAHH26 pKa = 5.97NNYY29 pKa = 8.84VSKK32 pKa = 10.59PVACYY37 pKa = 9.89HH38 pKa = 5.55GLEE41 pKa = 4.82FIVDD45 pKa = 3.82HH46 pKa = 6.48FKK48 pKa = 10.98PEE50 pKa = 3.92PSACLDD56 pKa = 3.2FHH58 pKa = 6.73EE59 pKa = 5.7FYY61 pKa = 11.06DD62 pKa = 4.29RR63 pKa = 11.84GYY65 pKa = 11.14LPTLIQGDD73 pKa = 4.32GPSQVRR79 pKa = 11.84SSAPPPDD86 pKa = 4.92GIFEE90 pKa = 4.71FISDD94 pKa = 4.25FSTDD98 pKa = 2.7IFEE101 pKa = 5.43SFDD104 pKa = 3.5EE105 pKa = 4.18MSMLDD110 pKa = 3.48AVRR113 pKa = 11.84WPTGTPSLQFFHH125 pKa = 7.0HH126 pKa = 6.18WRR128 pKa = 11.84NRR130 pKa = 11.84SPGYY134 pKa = 10.27NWTSKK139 pKa = 11.21SMIASEE145 pKa = 4.6LLTATTEE152 pKa = 4.0MCLCCAFPRR161 pKa = 11.84MHH163 pKa = 6.87GFIRR167 pKa = 11.84NIARR171 pKa = 11.84RR172 pKa = 11.84MDD174 pKa = 4.08LDD176 pKa = 4.37LKK178 pKa = 10.54LFPGGDD184 pKa = 3.09IVTEE188 pKa = 3.98ICHH191 pKa = 5.13IQCVKK196 pKa = 9.55MMKK199 pKa = 10.13AALIEE204 pKa = 4.35RR205 pKa = 11.84GEE207 pKa = 4.54DD208 pKa = 3.36KK209 pKa = 10.59PLSPVTEE216 pKa = 5.34LILTAMGEE224 pKa = 4.28YY225 pKa = 9.02TRR227 pKa = 11.84EE228 pKa = 3.74YY229 pKa = 11.15DD230 pKa = 3.8PMLKK234 pKa = 10.49EE235 pKa = 5.2LMDD238 pKa = 3.76EE239 pKa = 4.02AANYY243 pKa = 10.02FDD245 pKa = 4.41EE246 pKa = 4.37TLKK249 pKa = 11.18

Molecular weight: 28.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SHW0|A0A1S5SHW0_9VIRU Nucleoprotein OS=Anhanga virus OX=904722 PE=3 SV=1
MM1 pKa = 7.36SFSKK5 pKa = 10.44IALEE9 pKa = 4.16FGGEE13 pKa = 4.23SVDD16 pKa = 3.57VDD18 pKa = 4.95EE19 pKa = 4.6ISKK22 pKa = 9.62WVSEE26 pKa = 4.19FAYY29 pKa = 10.11QGYY32 pKa = 9.44DD33 pKa = 2.98ANRR36 pKa = 11.84VIEE39 pKa = 5.45LIQQYY44 pKa = 8.75GRR46 pKa = 11.84KK47 pKa = 9.01RR48 pKa = 11.84DD49 pKa = 2.99WKK51 pKa = 9.14MDD53 pKa = 3.64VKK55 pKa = 11.06RR56 pKa = 11.84MIVLALTRR64 pKa = 11.84GNKK67 pKa = 7.63PDD69 pKa = 3.45KK70 pKa = 9.8MRR72 pKa = 11.84KK73 pKa = 8.97KK74 pKa = 9.83MSAEE78 pKa = 3.77GVKK81 pKa = 10.2ILDD84 pKa = 3.76EE85 pKa = 4.22LVNVYY90 pKa = 9.83KK91 pKa = 10.78LKK93 pKa = 10.7SSSPGRR99 pKa = 11.84DD100 pKa = 3.29DD101 pKa = 3.72LTLSRR106 pKa = 11.84VAAAFAAWTCQATEE120 pKa = 3.77AVEE123 pKa = 4.72NYY125 pKa = 9.86MPVTGAAMDD134 pKa = 4.14EE135 pKa = 4.46VSPQYY140 pKa = 9.91PRR142 pKa = 11.84AMMHH146 pKa = 6.66PSFAGLIDD154 pKa = 4.19PQLKK158 pKa = 9.47PDD160 pKa = 3.45QRR162 pKa = 11.84EE163 pKa = 4.23LVVKK167 pKa = 10.44AHH169 pKa = 6.3SLFLIRR175 pKa = 11.84FSRR178 pKa = 11.84VINVALRR185 pKa = 11.84GRR187 pKa = 11.84PKK189 pKa = 10.69SEE191 pKa = 3.75VEE193 pKa = 3.99MSFSQPMNAAINSNFLTSDD212 pKa = 3.02NRR214 pKa = 11.84RR215 pKa = 11.84EE216 pKa = 3.94MLKK219 pKa = 10.71KK220 pKa = 10.65LGIVDD225 pKa = 3.72KK226 pKa = 11.21NGDD229 pKa = 3.72VVPSVIAAARR239 pKa = 11.84AYY241 pKa = 10.43DD242 pKa = 3.73KK243 pKa = 11.01EE244 pKa = 4.17II245 pKa = 4.68

Molecular weight: 27.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3949

245

2096

987.3

111.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.166 ± 0.838

2.836 ± 0.758

5.9 ± 0.468

6.812 ± 0.132

4.482 ± 0.469

5.9 ± 0.533

2.431 ± 0.264

7.116 ± 0.566

6.761 ± 0.258

8.483 ± 0.379

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.343 ± 0.547

4.204 ± 0.299

3.419 ± 0.551

3.191 ± 0.331

5.546 ± 0.357

9.445 ± 0.598

4.685 ± 0.388

6.077 ± 0.476

1.19 ± 0.043

3.013 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski