Drosophila sechellia (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; A

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4I0V5|B4I0V5_DROSE GM12668 OS=Drosophila sechellia OX=7238 GN=Dsec\GM12668 PE=4 SV=1
MM1 pKa = 7.94VYY3 pKa = 9.87IVHH6 pKa = 6.58NKK8 pKa = 10.06DD9 pKa = 5.28DD10 pKa = 4.93LDD12 pKa = 3.86QQLTLAEE19 pKa = 4.75DD20 pKa = 3.63KK21 pKa = 11.19LVVIDD26 pKa = 7.67FYY28 pKa = 11.88ADD30 pKa = 2.63WCGPCKK36 pKa = 10.27IIAPKK41 pKa = 10.59LEE43 pKa = 4.14EE44 pKa = 4.08LAEE47 pKa = 4.16QYY49 pKa = 10.83SDD51 pKa = 2.91RR52 pKa = 11.84VVVLKK57 pKa = 11.24VNVDD61 pKa = 3.48EE62 pKa = 5.41NEE64 pKa = 4.9DD65 pKa = 3.19ITVEE69 pKa = 4.09YY70 pKa = 9.83KK71 pKa = 10.66VNSMPTFVFIKK82 pKa = 10.57GGDD85 pKa = 3.65VLEE88 pKa = 4.76LLVGCNSDD96 pKa = 3.7KK97 pKa = 10.7LAKK100 pKa = 10.51LMEE103 pKa = 4.29EE104 pKa = 3.96HH105 pKa = 6.69AGVYY109 pKa = 7.38TSCVEE114 pKa = 5.19DD115 pKa = 4.67IIDD118 pKa = 4.46DD119 pKa = 3.9EE120 pKa = 5.32SIADD124 pKa = 3.81EE125 pKa = 4.33NAACSDD131 pKa = 3.74SDD133 pKa = 4.29NDD135 pKa = 4.63NDD137 pKa = 3.98NTNDD141 pKa = 3.69NTNDD145 pKa = 3.49NYY147 pKa = 11.45NDD149 pKa = 3.63NDD151 pKa = 3.97DD152 pKa = 4.19DD153 pKa = 6.47ADD155 pKa = 4.05VIFCEE160 pKa = 5.1VSAHH164 pKa = 7.09DD165 pKa = 3.85EE166 pKa = 4.28DD167 pKa = 5.52SVLEE171 pKa = 4.21HH172 pKa = 6.97

Molecular weight: 19.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4HXN7|B4HXN7_DROSE GM16005 OS=Drosophila sechellia OX=7238 GN=Dsec\GM16005 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.56LFIALSVLVAMASALPQFGGNANANANANANAQGGFGGFGGRR44 pKa = 11.84PGFGGGPGFGGGPGFGGSPGFAGRR68 pKa = 11.84PGFGAGPGFGGGPGFGGRR86 pKa = 11.84PGFGGGPGFGGGPGFGGRR104 pKa = 11.84PGFGGGPGFGGGSSSASASSSASASGGGRR133 pKa = 11.84GGAGSASASSSANASGNRR151 pKa = 11.84FGG153 pKa = 5.19

Molecular weight: 13.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16134

4

16138

7110928

8

14551

440.6

49.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.423 ± 0.024

1.934 ± 0.026

5.16 ± 0.017

6.348 ± 0.022

3.573 ± 0.015

6.1 ± 0.024

2.675 ± 0.014

4.868 ± 0.015

5.544 ± 0.023

9.166 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.39 ± 0.011

4.614 ± 0.014

5.477 ± 0.026

5.122 ± 0.028

5.644 ± 0.018

8.27 ± 0.027

5.598 ± 0.019

5.916 ± 0.017

1.032 ± 0.007

2.978 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski