Pseudomonas phage PspYZU08

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pijolavirus; Pseudomonas virus PspYZU08

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U7NN78|A0A2U7NN78_9CAUD Internal virion protein C OS=Pseudomonas phage PspYZU08 OX=1983557 GN=PspYZU08_41 PE=4 SV=1
MM1 pKa = 7.2EE2 pKa = 4.47VSMLSAIQYY11 pKa = 10.25YY12 pKa = 9.75IDD14 pKa = 4.54NPDD17 pKa = 4.38DD18 pKa = 4.0VPDD21 pKa = 4.16IPQASADD28 pKa = 3.84YY29 pKa = 10.55LAARR33 pKa = 11.84CNVSYY38 pKa = 10.44IQRR41 pKa = 11.84TGILDD46 pKa = 3.47QLRR49 pKa = 11.84KK50 pKa = 10.24EE51 pKa = 4.55GFSEE55 pKa = 3.82QALYY59 pKa = 10.41GFCEE63 pKa = 4.27GLSAVVEE70 pKa = 4.81IIEE73 pKa = 4.18LMQEE77 pKa = 3.82QRR79 pKa = 11.84HH80 pKa = 4.83LQEE83 pKa = 3.99

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U7NS43|A0A2U7NS43_9CAUD Uncharacterized protein OS=Pseudomonas phage PspYZU08 OX=1983557 GN=PspYZU08_26 PE=4 SV=1
MM1 pKa = 7.65VILHH5 pKa = 5.76SNKK8 pKa = 8.05YY9 pKa = 5.54TAPNVHH15 pKa = 6.09IRR17 pKa = 11.84TNKK20 pKa = 8.3HH21 pKa = 4.23TSQKK25 pKa = 9.93VLKK28 pKa = 9.84KK29 pKa = 10.25KK30 pKa = 9.93PQIHH34 pKa = 5.79KK35 pKa = 10.26QDD37 pKa = 3.17GKK39 pKa = 11.37VYY41 pKa = 10.17FVLQSDD47 pKa = 4.35LDD49 pKa = 3.69AARR52 pKa = 11.84QYY54 pKa = 10.3CQRR57 pKa = 11.84PLVSYY62 pKa = 10.08KK63 pKa = 11.03GNWPRR68 pKa = 11.84LAFLWARR75 pKa = 11.84VKK77 pKa = 10.72EE78 pKa = 4.29YY79 pKa = 11.54VNDD82 pKa = 3.73

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12330

56

1327

256.9

28.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.846 ± 0.414

0.843 ± 0.126

6.326 ± 0.205

6.886 ± 0.342

3.82 ± 0.199

8.062 ± 0.297

1.987 ± 0.181

5.053 ± 0.158

6.115 ± 0.34

7.989 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.177

3.82 ± 0.173

3.755 ± 0.186

4.931 ± 0.333

5.661 ± 0.248

5.702 ± 0.33

5.264 ± 0.182

6.334 ± 0.358

1.436 ± 0.147

3.285 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski