Hubei sobemo-like virus 35

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEI2|A0A1L3KEI2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 35 OX=1923222 PE=4 SV=1
MM1 pKa = 7.12PQRR4 pKa = 11.84YY5 pKa = 8.84DD6 pKa = 2.73WGQDD10 pKa = 3.09VSYY13 pKa = 11.15LEE15 pKa = 4.42EE16 pKa = 4.16LSKK19 pKa = 10.82EE20 pKa = 4.14YY21 pKa = 10.3SWPSFGHH28 pKa = 4.69TAEE31 pKa = 3.83IHH33 pKa = 4.95SLRR36 pKa = 11.84WHH38 pKa = 6.44LPLRR42 pKa = 11.84DD43 pKa = 3.64RR44 pKa = 11.84VEE46 pKa = 3.91QTPPQDD52 pKa = 3.2KK53 pKa = 10.27EE54 pKa = 4.35AILGEE59 pKa = 4.17TTKK62 pKa = 11.06YY63 pKa = 9.02VTDD66 pKa = 3.57AMAFIPEE73 pKa = 4.63DD74 pKa = 3.56FMQYY78 pKa = 7.54SHH80 pKa = 6.12YY81 pKa = 10.5VRR83 pKa = 11.84VVKK86 pKa = 10.22EE87 pKa = 4.29LEE89 pKa = 4.07WDD91 pKa = 3.61SSPGFPYY98 pKa = 9.77MYY100 pKa = 9.44EE101 pKa = 4.15YY102 pKa = 10.53PNNRR106 pKa = 11.84AFFRR110 pKa = 11.84VKK112 pKa = 10.58DD113 pKa = 3.88GLPDD117 pKa = 3.73PEE119 pKa = 5.04RR120 pKa = 11.84VDD122 pKa = 4.04ACWAMVQEE130 pKa = 4.28RR131 pKa = 11.84LKK133 pKa = 11.27NRR135 pKa = 11.84DD136 pKa = 3.08SDD138 pKa = 4.59PIRR141 pKa = 11.84LFIKK145 pKa = 10.25PEE147 pKa = 3.55PHH149 pKa = 5.44TKK151 pKa = 10.35KK152 pKa = 10.57KK153 pKa = 10.27IEE155 pKa = 4.05EE156 pKa = 3.86KK157 pKa = 10.2RR158 pKa = 11.84FRR160 pKa = 11.84LISSVSVVDD169 pKa = 4.32QILDD173 pKa = 3.28QMIFGFQNQAFLDD186 pKa = 4.18NHH188 pKa = 5.96HH189 pKa = 6.61HH190 pKa = 5.55TPVRR194 pKa = 11.84VGWGWMKK201 pKa = 10.88GGWNSVPKK209 pKa = 10.26SGMVACDD216 pKa = 3.15KK217 pKa = 10.85TGWDD221 pKa = 3.4WTVSAWLVDD230 pKa = 3.87LEE232 pKa = 4.45LEE234 pKa = 4.17LRR236 pKa = 11.84SRR238 pKa = 11.84LIFGTEE244 pKa = 3.38KK245 pKa = 10.98SLWEE249 pKa = 3.98DD250 pKa = 3.53LARR253 pKa = 11.84FRR255 pKa = 11.84YY256 pKa = 9.74HH257 pKa = 6.79EE258 pKa = 4.38LFIKK262 pKa = 10.72NEE264 pKa = 3.74FMTSGGLVFRR274 pKa = 11.84VRR276 pKa = 11.84QPGLMKK282 pKa = 10.34SGCVNTIVSNSLMQLLLHH300 pKa = 5.74VRR302 pKa = 11.84VSIEE306 pKa = 4.12LGEE309 pKa = 4.15QIHH312 pKa = 6.6NIWAMGDD319 pKa = 3.43DD320 pKa = 4.81TIQDD324 pKa = 3.7FMPFLPEE331 pKa = 3.67YY332 pKa = 10.96AEE334 pKa = 4.23LLGRR338 pKa = 11.84YY339 pKa = 9.05CILKK343 pKa = 9.8QVSGDD348 pKa = 3.68SEE350 pKa = 4.26FAGFRR355 pKa = 11.84WDD357 pKa = 3.21GDD359 pKa = 4.24YY360 pKa = 10.93IEE362 pKa = 4.89PLYY365 pKa = 10.08PEE367 pKa = 3.8KK368 pKa = 10.62HH369 pKa = 5.98AFQILHH375 pKa = 5.94VKK377 pKa = 10.26EE378 pKa = 3.8KK379 pKa = 10.29DD380 pKa = 3.22KK381 pKa = 11.44KK382 pKa = 10.72VFSLSYY388 pKa = 11.0NLLYY392 pKa = 10.01WKK394 pKa = 10.52SRR396 pKa = 11.84FLPYY400 pKa = 9.77IQRR403 pKa = 11.84LMPVPDD409 pKa = 3.19IGFTRR414 pKa = 11.84IWDD417 pKa = 3.79GEE419 pKa = 4.13

Molecular weight: 49.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEI2|A0A1L3KEI2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 35 OX=1923222 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 5.88SIKK5 pKa = 10.55QLALEE10 pKa = 4.21TLQNAGQLQLVGCLIILAVVVSNRR34 pKa = 11.84RR35 pKa = 11.84LITFPMVPLALWLILWDD52 pKa = 3.42TRR54 pKa = 11.84EE55 pKa = 4.14RR56 pKa = 11.84IVYY59 pKa = 9.54RR60 pKa = 11.84PVMPDD65 pKa = 3.5FSWVQAWVLMASEE78 pKa = 4.51SFQRR82 pKa = 11.84AFEE85 pKa = 4.03ASKK88 pKa = 10.52AYY90 pKa = 9.1MMGVIGVMVLLRR102 pKa = 11.84VWISLRR108 pKa = 11.84QSLKK112 pKa = 10.32EE113 pKa = 3.55VSLRR117 pKa = 11.84LRR119 pKa = 11.84GVTRR123 pKa = 11.84VQYY126 pKa = 10.03EE127 pKa = 3.83AMRR130 pKa = 11.84EE131 pKa = 3.88GSAFEE136 pKa = 4.18KK137 pKa = 10.93APIPACQVQICEE149 pKa = 4.22AGLFVNSHH157 pKa = 6.65LGYY160 pKa = 10.46GIRR163 pKa = 11.84VEE165 pKa = 4.16NCLVVPRR172 pKa = 11.84HH173 pKa = 5.56VLLAGKK179 pKa = 8.98WPTNKK184 pKa = 10.38LIISVPLRR192 pKa = 11.84PSMVLEE198 pKa = 3.94GRR200 pKa = 11.84MEE202 pKa = 4.06EE203 pKa = 4.2SQKK206 pKa = 10.59FDD208 pKa = 3.21LAYY211 pKa = 10.59FFLGEE216 pKa = 4.2AQWAALGAKK225 pKa = 9.59KK226 pKa = 9.34ATLTASSLQGRR237 pKa = 11.84ASCAGYY243 pKa = 10.74AGLSRR248 pKa = 11.84GDD250 pKa = 3.62LKK252 pKa = 10.54KK253 pKa = 9.41TNSVGQYY260 pKa = 10.37SYY262 pKa = 11.66GGSTKK267 pKa = 10.52AGYY270 pKa = 10.07SGAAYY275 pKa = 10.53VMQGQVAGIHH285 pKa = 6.18IGVDD289 pKa = 3.39CEE291 pKa = 4.67VNVGLSSRR299 pKa = 11.84LIAKK303 pKa = 8.97EE304 pKa = 3.91LEE306 pKa = 4.22YY307 pKa = 10.52LTVTEE312 pKa = 4.0ARR314 pKa = 11.84RR315 pKa = 11.84NHH317 pKa = 5.83GVSGLAAYY325 pKa = 7.47DD326 pKa = 3.71TPVQLVEE333 pKa = 4.58THH335 pKa = 7.36DD336 pKa = 3.16NWQDD340 pKa = 3.24LDD342 pKa = 3.83IEE344 pKa = 4.29EE345 pKa = 4.98LMYY348 pKa = 10.87ASRR351 pKa = 11.84SGDD354 pKa = 3.16EE355 pKa = 5.34RR356 pKa = 11.84ATEE359 pKa = 3.99WLRR362 pKa = 11.84KK363 pKa = 8.68NQHH366 pKa = 4.9KK367 pKa = 10.14HH368 pKa = 5.64KK369 pKa = 9.31KK370 pKa = 9.15HH371 pKa = 4.83PMYY374 pKa = 9.87WEE376 pKa = 4.17SMKK379 pKa = 10.63QALPSMDD386 pKa = 4.73PEE388 pKa = 4.34TLLQVQNAFNKK399 pKa = 10.39AFTFLQEE406 pKa = 4.34EE407 pKa = 4.84TCSCSGLLHH416 pKa = 6.39ACEE419 pKa = 4.27GKK421 pKa = 9.86RR422 pKa = 11.84GKK424 pKa = 10.8VIDD427 pKa = 3.92QRR429 pKa = 11.84PSTSRR434 pKa = 11.84VIGQSDD440 pKa = 4.07VPQEE444 pKa = 4.16AEE446 pKa = 3.83QGLTEE451 pKa = 4.44TPLHH455 pKa = 6.14RR456 pKa = 11.84ANMLKK461 pKa = 10.2RR462 pKa = 11.84IEE464 pKa = 4.15SLEE467 pKa = 4.12EE468 pKa = 3.62NEE470 pKa = 4.62KK471 pKa = 10.64ILVEE475 pKa = 4.45SVSNMDD481 pKa = 4.11EE482 pKa = 4.01NVKK485 pKa = 10.29DD486 pKa = 3.46LRR488 pKa = 11.84GNVTQIDD495 pKa = 3.69DD496 pKa = 5.24DD497 pKa = 3.78IYY499 pKa = 11.11KK500 pKa = 10.06IYY502 pKa = 10.77GRR504 pKa = 11.84IAFLEE509 pKa = 3.88EE510 pKa = 3.46RR511 pKa = 11.84LQNVEE516 pKa = 3.41LWAIDD521 pKa = 3.33RR522 pKa = 11.84GFANLPAALFSEE534 pKa = 5.31DD535 pKa = 3.38SPQVVAQKK543 pKa = 10.68RR544 pKa = 11.84RR545 pKa = 11.84TSKK548 pKa = 10.78NPAIPLVEE556 pKa = 5.04RR557 pKa = 11.84ISALKK562 pKa = 10.15LKK564 pKa = 10.56AYY566 pKa = 10.35GEE568 pKa = 4.37STKK571 pKa = 9.93PRR573 pKa = 11.84KK574 pKa = 9.3IVTPPLIRR582 pKa = 11.84KK583 pKa = 8.79VRR585 pKa = 11.84DD586 pKa = 3.85AIATRR591 pKa = 11.84TQSKK595 pKa = 9.73NVEE598 pKa = 4.1VEE600 pKa = 4.16LEE602 pKa = 4.35HH603 pKa = 8.46ISAAEE608 pKa = 3.78EE609 pKa = 3.86ARR611 pKa = 11.84EE612 pKa = 4.22KK613 pKa = 10.14MSSMTQKK620 pKa = 9.71QQRR623 pKa = 11.84EE624 pKa = 4.17RR625 pKa = 11.84YY626 pKa = 8.82KK627 pKa = 10.6EE628 pKa = 3.81LRR630 pKa = 11.84NKK632 pKa = 10.14QNLTPEE638 pKa = 4.24EE639 pKa = 4.11EE640 pKa = 4.29QFKK643 pKa = 9.55QQHH646 pKa = 5.71LEE648 pKa = 3.83SRR650 pKa = 11.84RR651 pKa = 11.84KK652 pKa = 8.95RR653 pKa = 11.84NARR656 pKa = 11.84RR657 pKa = 11.84RR658 pKa = 11.84TGKK661 pKa = 10.37KK662 pKa = 8.03STGNSRR668 pKa = 11.84STSSSSGKK676 pKa = 9.87NEE678 pKa = 4.08PSTSTQQ684 pKa = 2.85

Molecular weight: 76.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1103

419

684

551.5

63.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.437 ± 1.6

1.179 ± 0.125

4.442 ± 1.254

7.978 ± 0.325

3.626 ± 1.177

5.984 ± 0.01

2.267 ± 0.468

4.896 ± 0.199

5.984 ± 0.143

9.701 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.992 ± 0.196

3.354 ± 0.408

4.533 ± 0.669

5.168 ± 0.622

6.981 ± 0.434

7.525 ± 0.605

4.261 ± 0.515

7.072 ± 0.218

2.448 ± 0.768

3.173 ± 0.495
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski