Vicia cryptic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3R740|A3R740_9VIRU Coat protein OS=Vicia cryptic virus OX=54289 GN=CP PE=4 SV=1
MM1 pKa = 8.35DD2 pKa = 4.02YY3 pKa = 10.79LISAFNRR10 pKa = 11.84ITHH13 pKa = 6.2WFITPTNFEE22 pKa = 4.52YY23 pKa = 10.24IGNFSLPPGLLRR35 pKa = 11.84VNDD38 pKa = 3.63VAIANHH44 pKa = 6.43KK45 pKa = 8.33CTLEE49 pKa = 4.19RR50 pKa = 11.84SFHH53 pKa = 6.3TYY55 pKa = 10.89LFDD58 pKa = 4.72HH59 pKa = 7.13EE60 pKa = 4.96IKK62 pKa = 10.5RR63 pKa = 11.84IMIDD67 pKa = 3.03HH68 pKa = 6.73RR69 pKa = 11.84RR70 pKa = 11.84SDD72 pKa = 3.31ITEE75 pKa = 4.04DD76 pKa = 4.04SILEE80 pKa = 4.1DD81 pKa = 4.32FFAGDD86 pKa = 3.55FPYY89 pKa = 10.81FEE91 pKa = 4.8VPFDD95 pKa = 3.47EE96 pKa = 4.41HH97 pKa = 8.55VEE99 pKa = 4.07YY100 pKa = 10.84GLQCMADD107 pKa = 3.51AFRR110 pKa = 11.84PPRR113 pKa = 11.84PCRR116 pKa = 11.84PAHH119 pKa = 6.19ILDD122 pKa = 3.78VKK124 pKa = 10.61HH125 pKa = 6.44GYY127 pKa = 8.11PYY129 pKa = 10.43KK130 pKa = 10.06WNVNAEE136 pKa = 4.36PPFSTDD142 pKa = 3.38EE143 pKa = 4.07YY144 pKa = 11.14FLTQRR149 pKa = 11.84KK150 pKa = 6.66TFGEE154 pKa = 5.1FIRR157 pKa = 11.84MHH159 pKa = 6.57EE160 pKa = 4.21YY161 pKa = 10.71EE162 pKa = 5.95HH163 pKa = 7.09IDD165 pKa = 2.9KK166 pKa = 11.07DD167 pKa = 3.99DD168 pKa = 3.55FFRR171 pKa = 11.84RR172 pKa = 11.84HH173 pKa = 6.25PNLEE177 pKa = 3.7SHH179 pKa = 7.24DD180 pKa = 4.02FLRR183 pKa = 11.84TIVPPKK189 pKa = 10.3FGYY192 pKa = 10.37LKK194 pKa = 9.81STIFSWTRR202 pKa = 11.84RR203 pKa = 11.84WHH205 pKa = 6.57HH206 pKa = 6.35IIKK209 pKa = 10.58LGFTDD214 pKa = 4.06TTGLDD219 pKa = 3.64NNGYY223 pKa = 10.02LYY225 pKa = 11.06NRR227 pKa = 11.84FIFPMLLHH235 pKa = 6.66TKK237 pKa = 8.25TAIVKK242 pKa = 10.22KK243 pKa = 10.36DD244 pKa = 3.95DD245 pKa = 4.07PNKK248 pKa = 9.93MRR250 pKa = 11.84TIWGASKK257 pKa = 9.86PWIIADD263 pKa = 3.44TMFYY267 pKa = 10.22WEE269 pKa = 3.73YY270 pKa = 10.07QAWVKK275 pKa = 10.49HH276 pKa = 5.59NPGSTPMLWGFEE288 pKa = 4.25TFTGGWFRR296 pKa = 11.84LNQLLFCGLIRR307 pKa = 11.84RR308 pKa = 11.84SFITLDD314 pKa = 2.77WSRR317 pKa = 11.84FDD319 pKa = 3.15KK320 pKa = 10.78RR321 pKa = 11.84AYY323 pKa = 10.21FPLLRR328 pKa = 11.84KK329 pKa = 9.74IMYY332 pKa = 7.15TVKK335 pKa = 10.63SFLTFEE341 pKa = 4.03EE342 pKa = 6.21GYY344 pKa = 10.49VPTHH348 pKa = 6.91AAPNHH353 pKa = 5.48PQWNQDD359 pKa = 3.0KK360 pKa = 9.59TDD362 pKa = 3.37KK363 pKa = 11.32LEE365 pKa = 4.89RR366 pKa = 11.84LWLWTLEE373 pKa = 4.18NLFEE377 pKa = 4.94APIILPDD384 pKa = 2.89GRR386 pKa = 11.84MYY388 pKa = 10.65RR389 pKa = 11.84RR390 pKa = 11.84HH391 pKa = 5.78FAGIPSGLFITQLLDD406 pKa = 2.7SWYY409 pKa = 9.85NYY411 pKa = 9.3TMLATILHH419 pKa = 6.57ALGFNPSNCIIKK431 pKa = 10.11VQGDD435 pKa = 3.54DD436 pKa = 3.82SIIRR440 pKa = 11.84LNVLVPSEE448 pKa = 4.1RR449 pKa = 11.84HH450 pKa = 5.0DD451 pKa = 3.66HH452 pKa = 5.31LMSRR456 pKa = 11.84IVEE459 pKa = 3.9LAEE462 pKa = 4.18YY463 pKa = 9.65YY464 pKa = 10.69FNSIVNVEE472 pKa = 3.75KK473 pKa = 11.17SEE475 pKa = 3.71IRR477 pKa = 11.84NRR479 pKa = 11.84LNGCEE484 pKa = 3.81VLSYY488 pKa = 10.62RR489 pKa = 11.84NHH491 pKa = 6.44NGLPFRR497 pKa = 11.84DD498 pKa = 4.47EE499 pKa = 3.98IAMLAQFYY507 pKa = 7.91HH508 pKa = 6.12TKK510 pKa = 10.61ARR512 pKa = 11.84DD513 pKa = 3.58PTPEE517 pKa = 3.33ITMAQAIGFAYY528 pKa = 10.18ASCATHH534 pKa = 6.83TRR536 pKa = 11.84VLWVLEE542 pKa = 4.51DD543 pKa = 3.15IYY545 pKa = 11.41NYY547 pKa = 10.59YY548 pKa = 9.92RR549 pKa = 11.84DD550 pKa = 3.28QGYY553 pKa = 7.49TPNRR557 pKa = 11.84AGLTLTFGDD566 pKa = 4.55SPDD569 pKa = 3.29LTMPEE574 pKa = 4.14MPLDD578 pKa = 3.84HH579 pKa = 7.26FPTKK583 pKa = 10.63SEE585 pKa = 3.49IVRR588 pKa = 11.84YY589 pKa = 7.53LTCTNYY595 pKa = 10.3RR596 pKa = 11.84NEE598 pKa = 4.12AQNARR603 pKa = 11.84TWPRR607 pKa = 11.84TLFINAPAEE616 pKa = 3.97

Molecular weight: 72.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3R740|A3R740_9VIRU Coat protein OS=Vicia cryptic virus OX=54289 GN=CP PE=4 SV=1
MM1 pKa = 7.36EE2 pKa = 4.96AHH4 pKa = 6.24TPAADD9 pKa = 3.52VNGPNIPSGAVSQPEE24 pKa = 4.09IAPHH28 pKa = 5.56NQAAPNVSGAAQAIIAPTPARR49 pKa = 11.84KK50 pKa = 8.18RR51 pKa = 11.84TPHH54 pKa = 6.06GPIPAATSSGNTSAPALLEE73 pKa = 3.9LAAAYY78 pKa = 9.36PMYY81 pKa = 10.13TEE83 pKa = 4.07QRR85 pKa = 11.84RR86 pKa = 11.84SFNFFIPDD94 pKa = 3.38SQMMFHH100 pKa = 7.04SLGICDD106 pKa = 4.37SMMNSTDD113 pKa = 3.04RR114 pKa = 11.84FLRR117 pKa = 11.84SSPAWLPIVSQLYY130 pKa = 10.2ISVLWNVMIIRR141 pKa = 11.84ILSHH145 pKa = 6.05TGYY148 pKa = 10.85APSFTDD154 pKa = 4.84LLNTLTTDD162 pKa = 3.96LQIEE166 pKa = 4.57EE167 pKa = 4.74CMVPGPLVPFFQSLASINGPFDD189 pKa = 4.03WIGDD193 pKa = 3.7ITPALPGFDD202 pKa = 4.3SLWDD206 pKa = 3.52ATNFTPHH213 pKa = 6.4ASYY216 pKa = 10.46TRR218 pKa = 11.84FVPIPAILLDD228 pKa = 3.47QLYY231 pKa = 10.07YY232 pKa = 9.99CATYY236 pKa = 10.89VGVNQGDD243 pKa = 4.75LYY245 pKa = 10.28PTFTWYY251 pKa = 10.88RR252 pKa = 11.84NIFTRR257 pKa = 11.84TGANTPALLRR267 pKa = 11.84MGPNLCGSLFTTSNQFDD284 pKa = 3.55AARR287 pKa = 11.84TYY289 pKa = 9.62WRR291 pKa = 11.84ACFGTGFTRR300 pKa = 11.84VNVTAAAFTNYY311 pKa = 9.88LQLLGLRR318 pKa = 11.84SQTGEE323 pKa = 3.9PQTAWFQNVTMVMQKK338 pKa = 10.16YY339 pKa = 8.49AQHH342 pKa = 6.55FNGSVPLKK350 pKa = 10.8SIDD353 pKa = 3.47LTGIGAVALTGTPVNNTAVRR373 pKa = 11.84DD374 pKa = 3.58WLYY377 pKa = 10.5PPNANIEE384 pKa = 4.18PFTTGRR390 pKa = 11.84FNPRR394 pKa = 11.84RR395 pKa = 11.84EE396 pKa = 3.76IPAQLRR402 pKa = 11.84IRR404 pKa = 11.84FSHH407 pKa = 6.63SDD409 pKa = 3.38HH410 pKa = 6.46EE411 pKa = 4.78LEE413 pKa = 4.97LQAEE417 pKa = 4.5QYY419 pKa = 11.17AIAAHH424 pKa = 5.61TNIRR428 pKa = 11.84WAANIATQHH437 pKa = 5.67EE438 pKa = 4.36RR439 pKa = 11.84TAINPAHH446 pKa = 6.87LHH448 pKa = 6.17QGDD451 pKa = 4.3YY452 pKa = 11.31WNMTPFRR459 pKa = 11.84HH460 pKa = 5.94TGHH463 pKa = 7.36LGLKK467 pKa = 6.8TQYY470 pKa = 10.6AQLIASRR477 pKa = 11.84YY478 pKa = 7.81HH479 pKa = 5.22QLAANRR485 pKa = 11.84VDD487 pKa = 3.38

Molecular weight: 53.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1103

487

616

551.5

63.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.978 ± 2.193

1.179 ± 0.1

4.986 ± 0.853

4.261 ± 1.053

6.346 ± 0.802

5.077 ± 0.445

3.808 ± 0.345

6.528 ± 0.379

2.539 ± 1.136

8.704 ± 0.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.049

5.712 ± 0.297

7.072 ± 0.619

3.354 ± 1.176

6.256 ± 0.606

4.986 ± 0.64

8.069 ± 0.639

3.717 ± 0.258

2.448 ± 0.261

4.442 ± 0.494
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski