Dehalococcoidia bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Dehalococcoidia; unclassified Dehalococcoidia

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2903 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I1QVH3|A0A6I1QVH3_9CHLR ATP-binding cassette domain-containing protein OS=Dehalococcoidia bacterium OX=2026734 GN=GEU28_03685 PE=4 SV=1
MM1 pKa = 6.93MHH3 pKa = 7.24CNDD6 pKa = 3.74QNCAGGDD13 pKa = 3.7EE14 pKa = 5.5SITSPDD20 pKa = 3.31TADD23 pKa = 3.4VVGLDD28 pKa = 3.39TSLALDD34 pKa = 3.4ASGFPVVSYY43 pKa = 11.37QDD45 pKa = 3.57LTNGDD50 pKa = 4.36LKK52 pKa = 10.62ILRR55 pKa = 11.84CNDD58 pKa = 2.95QDD60 pKa = 3.92CFGNNEE66 pKa = 4.66SIASPDD72 pKa = 3.46SEE74 pKa = 4.57GVVGDD79 pKa = 3.95YY80 pKa = 10.73TSLALNARR88 pKa = 11.84GFPVVSYY95 pKa = 11.04FDD97 pKa = 3.63GTNGDD102 pKa = 4.5LKK104 pKa = 11.35VMHH107 pKa = 7.04CNDD110 pKa = 4.45PNCVLAVNEE119 pKa = 4.43WPRR122 pKa = 11.84CMGQLATIFAVPGQPTVGTNGKK144 pKa = 9.72DD145 pKa = 3.59VIQGTLGNDD154 pKa = 4.9FIRR157 pKa = 11.84GRR159 pKa = 11.84GGDD162 pKa = 3.94DD163 pKa = 3.73VICGLGGNDD172 pKa = 5.01LIAGNEE178 pKa = 4.17DD179 pKa = 3.51SDD181 pKa = 4.42TVNGGPGRR189 pKa = 11.84DD190 pKa = 2.91RR191 pKa = 11.84ALYY194 pKa = 10.62GGTPPVNVNLAAGTAIGQGNDD215 pKa = 3.07TLIAIEE221 pKa = 4.67DD222 pKa = 3.81ASGSSGDD229 pKa = 3.85DD230 pKa = 3.31GLRR233 pKa = 11.84TSNAANRR240 pKa = 11.84LWGFGGVDD248 pKa = 3.55TLVGLAGNDD257 pKa = 3.17ILTGGPGPDD266 pKa = 4.18HH267 pKa = 7.03LNGGPGTDD275 pKa = 3.86FCNGSVGLGDD285 pKa = 3.34TAVNCEE291 pKa = 4.03TTRR294 pKa = 11.84FVPP297 pKa = 4.34

Molecular weight: 30.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I1QL85|A0A6I1QL85_9CHLR Phosphotransferase OS=Dehalococcoidia bacterium OX=2026734 GN=GEU28_03000 PE=4 SV=1
MM1 pKa = 8.05PKK3 pKa = 9.18RR4 pKa = 11.84TWQPKK9 pKa = 8.36RR10 pKa = 11.84LPRR13 pKa = 11.84KK14 pKa = 9.06RR15 pKa = 11.84KK16 pKa = 8.83HH17 pKa = 5.84GFLARR22 pKa = 11.84MATRR26 pKa = 11.84GGRR29 pKa = 11.84RR30 pKa = 11.84VVRR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84RR41 pKa = 11.84QLAVV45 pKa = 3.09

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2903

0

2903

842137

26

1566

290.1

31.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.717 ± 0.06

0.84 ± 0.014

6.062 ± 0.041

6.751 ± 0.045

3.492 ± 0.031

8.956 ± 0.045

1.961 ± 0.022

4.755 ± 0.036

2.203 ± 0.031

10.067 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.118 ± 0.019

2.412 ± 0.024

5.409 ± 0.036

3.079 ± 0.026

7.648 ± 0.052

5.699 ± 0.031

5.095 ± 0.032

8.058 ± 0.044

1.339 ± 0.018

2.335 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski