Escherichia phage phiEB49

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Rogunavirinae; Lindendrivevirus; Escherichia virus phiEB49

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8UBS5|F8UBS5_9CAUD Gp15 OS=Escherichia phage phiEB49 OX=1048207 PE=4 SV=1
MM1 pKa = 7.53NLPGLDD7 pKa = 3.81FDD9 pKa = 4.08EE10 pKa = 4.83SKK12 pKa = 11.23YY13 pKa = 10.09PVVFFGEE20 pKa = 4.02EE21 pKa = 4.38PYY23 pKa = 10.92RR24 pKa = 11.84KK25 pKa = 9.22IPFSDD30 pKa = 4.66WIPPDD35 pKa = 3.57YY36 pKa = 11.16LDD38 pKa = 3.51VLVQIEE44 pKa = 4.29VGKK47 pKa = 10.82AEE49 pKa = 4.21LMYY52 pKa = 10.31YY53 pKa = 10.4SPGCNMMMPSEE64 pKa = 4.22YY65 pKa = 10.49SPAVAALEE73 pKa = 4.41TEE75 pKa = 4.58FLDD78 pKa = 3.71YY79 pKa = 9.63WLKK82 pKa = 10.52EE83 pKa = 3.93IKK85 pKa = 10.33

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8UBT3|F8UBT3_9CAUD Gp23 OS=Escherichia phage phiEB49 OX=1048207 PE=4 SV=1
MM1 pKa = 8.22SEE3 pKa = 3.57IDD5 pKa = 4.26KK6 pKa = 9.8RR7 pKa = 11.84TLNGNNGTIRR17 pKa = 11.84TEE19 pKa = 3.92DD20 pKa = 3.06KK21 pKa = 10.74KK22 pKa = 10.7PRR24 pKa = 11.84KK25 pKa = 9.27RR26 pKa = 11.84PSGYY30 pKa = 10.17YY31 pKa = 8.95VLKK34 pKa = 10.9DD35 pKa = 3.51EE36 pKa = 4.52VRR38 pKa = 11.84AGLRR42 pKa = 11.84ARR44 pKa = 11.84LEE46 pKa = 4.11IVLDD50 pKa = 4.33FYY52 pKa = 11.62GSKK55 pKa = 11.29ANMAKK60 pKa = 9.48QLKK63 pKa = 7.09VTRR66 pKa = 11.84QAVEE70 pKa = 3.54EE71 pKa = 4.23WFNRR75 pKa = 11.84GMMSARR81 pKa = 11.84GAQLAHH87 pKa = 5.93NRR89 pKa = 11.84YY90 pKa = 9.16KK91 pKa = 10.35RR92 pKa = 11.84TGEE95 pKa = 3.84GFRR98 pKa = 11.84ATFCRR103 pKa = 11.84PDD105 pKa = 3.38LQFDD109 pKa = 4.36GNGKK113 pKa = 8.92PLTLRR118 pKa = 11.84CKK120 pKa = 9.49KK121 pKa = 9.98RR122 pKa = 11.84HH123 pKa = 4.94MLRR126 pKa = 11.84VVTDD130 pKa = 3.75AEE132 pKa = 4.42LATKK136 pKa = 9.15PEE138 pKa = 4.04CRR140 pKa = 11.84SWRR143 pKa = 11.84KK144 pKa = 9.4IKK146 pKa = 10.36AANEE150 pKa = 3.74AARR153 pKa = 11.84KK154 pKa = 9.56AKK156 pKa = 9.88EE157 pKa = 3.61

Molecular weight: 18.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14022

34

1129

189.5

21.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.565 ± 0.573

1.277 ± 0.181

6.262 ± 0.261

6.383 ± 0.333

3.894 ± 0.206

7.624 ± 0.337

1.505 ± 0.205

6.54 ± 0.268

6.654 ± 0.492

6.882 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.296

5.392 ± 0.294

2.953 ± 0.194

3.723 ± 0.394

4.643 ± 0.232

6.875 ± 0.374

5.784 ± 0.332

7.174 ± 0.262

1.234 ± 0.117

3.865 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski