Desulfuribacillus alkaliarsenatis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillales incertae sedis; Desulfuribacillus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2879 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1E5G3B0|A0A1E5G3B0_9BACL Uncharacterized protein OS=Desulfuribacillus alkaliarsenatis OX=766136 GN=BHF68_05585 PE=4 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84FTKK5 pKa = 10.18KK6 pKa = 10.13VVSICVIFMFILGTALPTGLAAEE29 pKa = 4.13QQIQINFNGQSYY41 pKa = 10.09NADD44 pKa = 3.92FYY46 pKa = 11.78LEE48 pKa = 5.16DD49 pKa = 6.1DD50 pKa = 3.14ITYY53 pKa = 10.56INVDD57 pKa = 3.85SLKK60 pKa = 10.36KK61 pKa = 10.39IPGLEE66 pKa = 4.03VNEE69 pKa = 4.1YY70 pKa = 10.79NYY72 pKa = 10.68VPLRR76 pKa = 11.84AFFEE80 pKa = 4.69GQGATVTWNAQSKK93 pKa = 10.61VIDD96 pKa = 3.71ISWRR100 pKa = 11.84EE101 pKa = 4.05TNDD104 pKa = 2.24GWTANDD110 pKa = 3.58LVVKK114 pKa = 10.69AEE116 pKa = 4.21EE117 pKa = 4.0VLKK120 pKa = 10.66EE121 pKa = 3.85YY122 pKa = 9.39NTYY125 pKa = 10.9RR126 pKa = 11.84MAGDD130 pKa = 3.49GTIDD134 pKa = 3.54ISISGNDD141 pKa = 3.49AADD144 pKa = 3.78AFPFDD149 pKa = 4.0GMTLAIEE156 pKa = 4.88GSFSQEE162 pKa = 3.42PLAMYY167 pKa = 7.52MKK169 pKa = 9.48QTMTMPINFEE179 pKa = 4.05EE180 pKa = 4.19MGLTEE185 pKa = 4.95EE186 pKa = 4.58EE187 pKa = 4.13LALMGMDD194 pKa = 3.1TSMVTEE200 pKa = 4.5MVWKK204 pKa = 10.65DD205 pKa = 3.1NAIYY209 pKa = 10.46QRR211 pKa = 11.84ILPLQEE217 pKa = 3.3QWIVNNIEE225 pKa = 4.22DD226 pKa = 4.69LGMSDD231 pKa = 3.85TFNDD235 pKa = 4.28LLQMTPQQNLEE246 pKa = 4.11LMTKK250 pKa = 10.16YY251 pKa = 10.74GVFYY255 pKa = 10.52TFGDD259 pKa = 3.85DD260 pKa = 3.91VEE262 pKa = 4.54VDD264 pKa = 3.82GIEE267 pKa = 4.33YY268 pKa = 10.62YY269 pKa = 10.7VVNSTLSADD278 pKa = 3.67TFRR281 pKa = 11.84NLMEE285 pKa = 5.99DD286 pKa = 3.25MFDD289 pKa = 4.51DD290 pKa = 4.96LMPPEE295 pKa = 5.0LLTEE299 pKa = 4.29EE300 pKa = 4.15SDD302 pKa = 3.8EE303 pKa = 4.31VSVMIDD309 pKa = 3.32EE310 pKa = 4.53IFANINMNFYY320 pKa = 10.54QQTLINKK327 pKa = 7.44EE328 pKa = 4.12TLTTDD333 pKa = 3.68FLIYY337 pKa = 10.94DD338 pKa = 3.91MVMSLALADD347 pKa = 3.98MFSQEE352 pKa = 5.89DD353 pKa = 4.33GLQEE357 pKa = 4.16DD358 pKa = 4.87APEE361 pKa = 4.24SMLMSMSGVYY371 pKa = 9.94HH372 pKa = 7.17LYY374 pKa = 10.89DD375 pKa = 3.7FGVEE379 pKa = 3.9IEE381 pKa = 4.82LPDD384 pKa = 3.48VSDD387 pKa = 5.62AITQEE392 pKa = 4.36EE393 pKa = 4.92YY394 pKa = 10.2MEE396 pKa = 3.92QQMQLFEE403 pKa = 4.34SLEE406 pKa = 4.21PEE408 pKa = 4.41VQQ410 pKa = 3.1

Molecular weight:
46.83 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1E5G318|A0A1E5G318_9BACL Arsenic-transporting ATPase OS=Desulfuribacillus alkaliarsenatis OX=766136 GN=BHF68_04430 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84KK13 pKa = 8.71KK14 pKa = 8.74VHH16 pKa = 5.61GFRR19 pKa = 11.84KK20 pKa = 9.89RR21 pKa = 11.84MSSSSGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.38KK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.71VLSAA44 pKa = 4.05

Molecular weight:
5.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2879

0

2879

915468

37

2151

318.0

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.119 ± 0.047

0.867 ± 0.019

5.354 ± 0.036

7.215 ± 0.049

4.078 ± 0.033

6.485 ± 0.042

1.94 ± 0.021

8.811 ± 0.047

6.574 ± 0.052

9.604 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.022

4.895 ± 0.038

3.373 ± 0.022

3.985 ± 0.035

4.213 ± 0.032

5.807 ± 0.029

5.366 ± 0.028

7.074 ± 0.036

0.886 ± 0.015

3.719 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski