Avon-Heathcote Estuary associated circular virus 24

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IB82|A0A0C5IB82_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 24 OX=1618248 PE=4 SV=1
MM1 pKa = 7.04AQSRR5 pKa = 11.84SWCFTLNNYY14 pKa = 7.96VQADD18 pKa = 3.79IDD20 pKa = 3.6RR21 pKa = 11.84LAAFGEE27 pKa = 4.5TDD29 pKa = 3.28DD30 pKa = 3.9CTYY33 pKa = 11.26LVFGKK38 pKa = 10.34EE39 pKa = 4.14VGEE42 pKa = 4.34SGTPHH47 pKa = 6.17LQGFAIFPRR56 pKa = 11.84KK57 pKa = 9.44LRR59 pKa = 11.84LRR61 pKa = 11.84AVKK64 pKa = 10.13SHH66 pKa = 6.75IGNGAHH72 pKa = 6.93LEE74 pKa = 4.19CARR77 pKa = 11.84GSPLQASDD85 pKa = 3.65YY86 pKa = 10.15CKK88 pKa = 10.49KK89 pKa = 10.86DD90 pKa = 2.89GDD92 pKa = 3.88FTEE95 pKa = 5.15FGSFGGVRR103 pKa = 11.84PGLSGRR109 pKa = 11.84FGQFVEE115 pKa = 4.62WLDD118 pKa = 3.45EE119 pKa = 4.32HH120 pKa = 7.04YY121 pKa = 11.0KK122 pKa = 10.29EE123 pKa = 5.27GNVHH127 pKa = 5.33QPSRR131 pKa = 11.84ALIAATWPDD140 pKa = 3.61LYY142 pKa = 11.33VRR144 pKa = 11.84YY145 pKa = 8.32HH146 pKa = 5.87TKK148 pKa = 10.45LFEE151 pKa = 4.34LVGVLAPKK159 pKa = 10.27PILQEE164 pKa = 3.89GDD166 pKa = 3.16PNAWQTTLIEE176 pKa = 4.5ALGLDD181 pKa = 3.56PDD183 pKa = 3.68DD184 pKa = 4.49RR185 pKa = 11.84KK186 pKa = 10.64IYY188 pKa = 10.2FYY190 pKa = 11.39VDD192 pKa = 3.07EE193 pKa = 4.94TGGSGKK199 pKa = 10.07SWLTRR204 pKa = 11.84YY205 pKa = 10.52LMTSRR210 pKa = 11.84DD211 pKa = 3.77DD212 pKa = 3.69VQALSIGKK220 pKa = 9.51RR221 pKa = 11.84DD222 pKa = 4.82DD223 pKa = 3.07IAHH226 pKa = 7.1AIDD229 pKa = 3.48VTKK232 pKa = 10.62KK233 pKa = 9.76VFLFNVPRR241 pKa = 11.84TQMEE245 pKa = 4.25FLQYY249 pKa = 10.86SILEE253 pKa = 4.2SLKK256 pKa = 10.54DD257 pKa = 3.68RR258 pKa = 11.84MVMSPKK264 pKa = 9.37YY265 pKa = 9.59NSMMKK270 pKa = 9.94VLHH273 pKa = 6.07SVPHH277 pKa = 5.07VVVFSNEE284 pKa = 3.75EE285 pKa = 3.67PDD287 pKa = 3.73RR288 pKa = 11.84TKK290 pKa = 10.55LTADD294 pKa = 3.88RR295 pKa = 11.84FAVTHH300 pKa = 5.9IRR302 pKa = 11.84NII304 pKa = 3.59

Molecular weight: 34.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IB82|A0A0C5IB82_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 24 OX=1618248 PE=4 SV=1
MM1 pKa = 7.19SAVVTYY7 pKa = 9.64GPRR10 pKa = 11.84AARR13 pKa = 11.84FAMRR17 pKa = 11.84TYY19 pKa = 10.16RR20 pKa = 11.84NRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84VATYY28 pKa = 10.84ARR30 pKa = 11.84GARR33 pKa = 11.84MALNLARR40 pKa = 11.84NKK42 pKa = 10.03RR43 pKa = 11.84VRR45 pKa = 11.84QAVGQAYY52 pKa = 9.87RR53 pKa = 11.84RR54 pKa = 11.84VKK56 pKa = 10.12RR57 pKa = 11.84RR58 pKa = 11.84KK59 pKa = 7.27TNSFKK64 pKa = 10.91GRR66 pKa = 11.84APRR69 pKa = 11.84SNKK72 pKa = 8.86QRR74 pKa = 11.84SNTQNNQQLVVNHH87 pKa = 6.86LEE89 pKa = 4.06STIPSWPPAASDD101 pKa = 3.75SDD103 pKa = 3.77PKK105 pKa = 10.91QGFRR109 pKa = 11.84RR110 pKa = 11.84GNFIMLDD117 pKa = 3.45ALKK120 pKa = 10.94GCWTFKK126 pKa = 10.69NSGRR130 pKa = 11.84YY131 pKa = 8.12PVTVHH136 pKa = 6.25FACVQSVADD145 pKa = 4.1ANANAKK151 pKa = 9.57IDD153 pKa = 3.88FFSNPGGTDD162 pKa = 2.82KK163 pKa = 11.63YY164 pKa = 11.28KK165 pKa = 11.25DD166 pKa = 3.66FVDD169 pKa = 3.77SKK171 pKa = 11.58NVFDD175 pKa = 5.45IDD177 pKa = 4.51KK178 pKa = 10.97DD179 pKa = 3.99CLGLNKK185 pKa = 10.43NKK187 pKa = 10.17FNILFHH193 pKa = 6.32KK194 pKa = 10.21KK195 pKa = 9.39IYY197 pKa = 10.24LEE199 pKa = 4.29EE200 pKa = 5.42GEE202 pKa = 4.98HH203 pKa = 6.69VDD205 pKa = 3.38GANIRR210 pKa = 11.84TYY212 pKa = 11.01SLSEE216 pKa = 3.94SKK218 pKa = 10.93YY219 pKa = 7.82MKK221 pKa = 9.55TIRR224 pKa = 11.84VYY226 pKa = 10.8RR227 pKa = 11.84PIKK230 pKa = 10.43KK231 pKa = 10.33NIEE234 pKa = 4.13FEE236 pKa = 4.33NKK238 pKa = 8.5STLEE242 pKa = 3.61PLKK245 pKa = 10.6NIKK248 pKa = 9.41YY249 pKa = 9.77LYY251 pKa = 9.21WFVPRR256 pKa = 11.84YY257 pKa = 8.98STDD260 pKa = 3.1QPLAGQTKK268 pKa = 8.74GEE270 pKa = 4.01RR271 pKa = 11.84AVTVDD276 pKa = 3.05HH277 pKa = 6.97TDD279 pKa = 2.92SVLYY283 pKa = 10.06RR284 pKa = 11.84DD285 pKa = 3.98RR286 pKa = 11.84LL287 pKa = 3.62

Molecular weight: 33.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

591

287

304

295.5

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.276 ± 0.468

1.184 ± 0.088

6.43 ± 0.542

4.23 ± 0.694

5.245 ± 0.233

6.43 ± 0.763

2.538 ± 0.505

4.061 ± 0.145

7.107 ± 1.018

7.614 ± 1.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.183

5.245 ± 1.536

4.399 ± 0.138

3.553 ± 0.044

8.122 ± 1.258

6.261 ± 0.007

5.584 ± 0.006

7.107 ± 0.088

1.354 ± 0.196

4.23 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski