Burkholderia virus BcepC6B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Ryyoungvirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6J1R9|Q6J1R9_9CAUD Gp08 OS=Burkholderia virus BcepC6B OX=279280 GN=g08 PE=4 SV=1
MM1 pKa = 7.36YY2 pKa = 9.86IDD4 pKa = 4.17SLLEE8 pKa = 3.62FSRR11 pKa = 11.84AQALSASGASTNIIDD26 pKa = 4.68LGSDD30 pKa = 3.13RR31 pKa = 11.84DD32 pKa = 3.74IGPGRR37 pKa = 11.84PLWVVVSPSVAADD50 pKa = 3.3NTTGDD55 pKa = 3.63EE56 pKa = 4.49TYY58 pKa = 10.76SIALQTDD65 pKa = 3.44DD66 pKa = 4.13NAAFSSPTTIATVAPAAASFGAGVRR91 pKa = 11.84VVIGMPFANEE101 pKa = 3.6RR102 pKa = 11.84YY103 pKa = 9.62LRR105 pKa = 11.84LNYY108 pKa = 9.55TLGGTTPSVTLNAFLTDD125 pKa = 3.39QDD127 pKa = 4.0PASWQAYY134 pKa = 7.97PDD136 pKa = 4.95GIAA139 pKa = 4.07

Molecular weight: 14.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6J1P9|Q6J1P9_9CAUD Gp28 OS=Burkholderia virus BcepC6B OX=279280 GN=g28 PE=4 SV=1
MM1 pKa = 7.29LRR3 pKa = 11.84GEE5 pKa = 4.49VRR7 pKa = 11.84HH8 pKa = 5.75VMQTIKK14 pKa = 10.52PRR16 pKa = 11.84ARR18 pKa = 11.84QLSAHH23 pKa = 5.44NWYY26 pKa = 8.15VTNRR30 pKa = 11.84DD31 pKa = 3.71GISAFDD37 pKa = 3.65TTLDD41 pKa = 3.59GALALYY47 pKa = 7.77FRR49 pKa = 11.84CVLHH53 pKa = 6.18TMMEE57 pKa = 4.15RR58 pKa = 11.84RR59 pKa = 11.84PP60 pKa = 3.57

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

13174

49

888

286.4

30.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.489 ± 0.729

0.903 ± 0.131

6.391 ± 0.267

5.131 ± 0.333

2.884 ± 0.192

8.479 ± 0.37

1.685 ± 0.159

4.319 ± 0.163

3.841 ± 0.28

8.335 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.15

3.484 ± 0.286

5.184 ± 0.24

4.19 ± 0.413

6.748 ± 0.402

5.48 ± 0.381

6.414 ± 0.426

6.627 ± 0.254

1.731 ± 0.165

2.361 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski