Legionella wadsworthii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A378LUK2|A0A378LUK2_9GAMM 3' 5'-cyclic-nucleotide phosphodiesterase OS=Legionella wadsworthii OX=28088 GN=cpdA_1 PE=4 SV=1
MM1 pKa = 7.43AAIDD5 pKa = 4.18ISPTTNDD12 pKa = 2.22IRR14 pKa = 11.84FSVSAADD21 pKa = 4.9KK22 pKa = 9.9VAEE25 pKa = 4.56LIKK28 pKa = 10.88EE29 pKa = 4.16EE30 pKa = 4.34EE31 pKa = 4.22NPNLNLRR38 pKa = 11.84VSITGGGCSGFQYY51 pKa = 10.7GFSFDD56 pKa = 3.71EE57 pKa = 5.92DD58 pKa = 3.53INEE61 pKa = 4.74DD62 pKa = 3.57DD63 pKa = 4.06TVVVQYY69 pKa = 10.89CSDD72 pKa = 3.38GVSSVRR78 pKa = 11.84LLVDD82 pKa = 3.38SMSYY86 pKa = 10.53QYY88 pKa = 11.57LHH90 pKa = 7.17DD91 pKa = 4.61AEE93 pKa = 4.55IDD95 pKa = 3.89YY96 pKa = 10.99VQGIQGEE103 pKa = 4.42QFVIRR108 pKa = 11.84NPNAKK113 pKa = 6.55TTCGCGSSFSIDD125 pKa = 4.83DD126 pKa = 3.84EE127 pKa = 5.58DD128 pKa = 5.54DD129 pKa = 3.33NN130 pKa = 5.29

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A378LT35|A0A378LT35_9GAMM Uncharacterized protein OS=Legionella wadsworthii OX=28088 GN=NCTC11532_01179 PE=4 SV=1
MM1 pKa = 7.44TNLNRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84HH9 pKa = 5.26MNLLIQARR17 pKa = 11.84RR18 pKa = 11.84QKK20 pKa = 10.78LFSITKK26 pKa = 9.59NVGNAKK32 pKa = 10.23

Molecular weight: 3.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3179

0

3179

1046041

29

2944

329.0

37.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.217 ± 0.043

1.198 ± 0.018

4.71 ± 0.031

6.097 ± 0.049

4.692 ± 0.032

6.019 ± 0.049

2.606 ± 0.025

7.666 ± 0.039

6.484 ± 0.044

10.891 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.021

5.005 ± 0.032

4.146 ± 0.03

4.496 ± 0.032

4.048 ± 0.036

6.745 ± 0.034

5.143 ± 0.034

5.728 ± 0.033

1.156 ± 0.017

3.515 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski