Bacillus phage BCD7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Becedseptimavirus; Bacillus virus BCD7

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 140 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9PUK5|J9PUK5_9CAUD Uncharacterized protein OS=Bacillus phage BCD7 OX=1136534 GN=BCD7_0009 PE=4 SV=1
MM1 pKa = 7.53TYY3 pKa = 10.02IEE5 pKa = 4.82NVDD8 pKa = 4.09FTFVNPNDD16 pKa = 3.26IEE18 pKa = 4.45YY19 pKa = 10.37FDD21 pKa = 3.5TSYY24 pKa = 10.96IINYY28 pKa = 9.36HH29 pKa = 5.53NDD31 pKa = 2.69KK32 pKa = 10.0MDD34 pKa = 4.36YY35 pKa = 10.05IVYY38 pKa = 10.43ANCEE42 pKa = 3.69QDD44 pKa = 5.1ALDD47 pKa = 4.1ALIDD51 pKa = 3.74FLVARR56 pKa = 11.84EE57 pKa = 4.13EE58 pKa = 4.14TGLLYY63 pKa = 10.28TRR65 pKa = 11.84EE66 pKa = 4.58IIEE69 pKa = 4.34EE70 pKa = 4.22WNDD73 pKa = 4.15DD74 pKa = 3.14IDD76 pKa = 6.05DD77 pKa = 4.01YY78 pKa = 12.04VSAGNEE84 pKa = 3.12GRR86 pKa = 11.84YY87 pKa = 8.68YY88 pKa = 11.05VCTAYY93 pKa = 10.76LRR95 pKa = 11.84ITKK98 pKa = 10.28RR99 pKa = 11.84EE100 pKa = 3.95DD101 pKa = 2.79

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9PUK9|J9PUK9_9CAUD Uncharacterized protein OS=Bacillus phage BCD7 OX=1136534 GN=BCD7_0029 PE=4 SV=1
MM1 pKa = 8.13LFMNVFATPKK11 pKa = 9.85GAGGGGGRR19 pKa = 11.84AQTPAGGGLPRR30 pKa = 11.84TAPRR34 pKa = 11.84TLGGGGSASAGRR46 pKa = 11.84QARR49 pKa = 11.84QARR52 pKa = 11.84ASRR55 pKa = 11.84AKK57 pKa = 10.46LPRR60 pKa = 11.84TAPRR64 pKa = 11.84RR65 pKa = 11.84MGGRR69 pKa = 3.39

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

140

0

140

28075

41

1736

200.5

22.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.333 ± 0.328

0.73 ± 0.079

5.98 ± 0.215

8.67 ± 0.417

4.178 ± 0.132

6.76 ± 0.353

1.959 ± 0.093

6.483 ± 0.216

8.655 ± 0.348

7.437 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.195 ± 0.161

5.065 ± 0.194

3.266 ± 0.12

3.758 ± 0.166

4.88 ± 0.228

4.997 ± 0.161

5.927 ± 0.3

6.753 ± 0.158

1.104 ± 0.064

3.868 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski