Hubei virga-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJT4|A0A1L3KJT4_9VIRU Non-structural protein 2 OS=Hubei virga-like virus 15 OX=1923330 PE=4 SV=1
MM1 pKa = 7.48LVFNFLLLFTSTVAVHH17 pKa = 6.7RR18 pKa = 11.84PIPNYY23 pKa = 10.92DD24 pKa = 3.62PDD26 pKa = 3.99DD27 pKa = 4.62KK28 pKa = 11.27FWFSPPSADD37 pKa = 3.93AGKK40 pKa = 8.45LTKK43 pKa = 9.86MYY45 pKa = 10.21DD46 pKa = 3.15LHH48 pKa = 8.67GGFKK52 pKa = 8.86WCHH55 pKa = 5.1GSVLRR60 pKa = 11.84YY61 pKa = 9.62GFWEE65 pKa = 4.37GLNAFTIYY73 pKa = 9.83MYY75 pKa = 10.81LEE77 pKa = 4.54KK78 pKa = 10.72DD79 pKa = 3.68LYY81 pKa = 10.67RR82 pKa = 11.84LRR84 pKa = 11.84NKK86 pKa = 8.43VTIHH90 pKa = 6.17TGQYY94 pKa = 9.5VYY96 pKa = 11.33SLVGAHH102 pKa = 7.31LLTNDD107 pKa = 4.27RR108 pKa = 11.84EE109 pKa = 4.39FEE111 pKa = 4.22NFNSKK116 pKa = 10.4HH117 pKa = 6.04SLACFPDD124 pKa = 3.39TGLPKK129 pKa = 9.77CTLRR133 pKa = 11.84EE134 pKa = 3.66ICKK137 pKa = 10.32ARR139 pKa = 11.84TGKK142 pKa = 10.56KK143 pKa = 9.58PFPLVGTCEE152 pKa = 4.09NKK154 pKa = 10.25DD155 pKa = 3.35FRR157 pKa = 11.84IVEE160 pKa = 4.29RR161 pKa = 11.84GADD164 pKa = 3.77FDD166 pKa = 4.46PQCVCYY172 pKa = 10.45GDD174 pKa = 4.95CDD176 pKa = 3.94HH177 pKa = 7.46DD178 pKa = 4.57CEE180 pKa = 5.51HH181 pKa = 6.47STLHH185 pKa = 5.95NYY187 pKa = 9.99KK188 pKa = 10.56GIEE191 pKa = 3.86FSLGYY196 pKa = 9.92RR197 pKa = 11.84FNSKK201 pKa = 10.35PPLKK205 pKa = 10.74VSVDD209 pKa = 3.65RR210 pKa = 11.84YY211 pKa = 10.88GVGCIDD217 pKa = 4.0GLTSLAHH224 pKa = 6.38TYY226 pKa = 8.1WQTANYY232 pKa = 9.2HH233 pKa = 5.79PAYY236 pKa = 9.7LANSGVLQTFRR247 pKa = 11.84LRR249 pKa = 11.84KK250 pKa = 9.24EE251 pKa = 4.2FEE253 pKa = 4.15DD254 pKa = 4.65LRR256 pKa = 11.84PTVLNVTISNRR267 pKa = 11.84YY268 pKa = 7.92VCDD271 pKa = 3.45DD272 pKa = 3.24AMSRR276 pKa = 11.84RR277 pKa = 11.84YY278 pKa = 9.07HH279 pKa = 6.55ALDD282 pKa = 3.5GVVIYY287 pKa = 7.3PTAIDD292 pKa = 3.6CTEE295 pKa = 4.04FVPFDD300 pKa = 3.62FHH302 pKa = 7.27TLICKK307 pKa = 10.0DD308 pKa = 3.28ALFVPMCPTEE318 pKa = 3.99VFKK321 pKa = 11.26PMVARR326 pKa = 11.84YY327 pKa = 9.19RR328 pKa = 11.84YY329 pKa = 10.24YY330 pKa = 10.78DD331 pKa = 3.26ITNHH335 pKa = 5.43RR336 pKa = 11.84RR337 pKa = 11.84VVGALDD343 pKa = 3.7LVGAIQNQTGDD354 pKa = 3.37TMVDD358 pKa = 2.95HH359 pKa = 7.0FFDD362 pKa = 3.98HH363 pKa = 6.86FAGNATRR370 pKa = 11.84HH371 pKa = 4.9HH372 pKa = 5.53VVRR375 pKa = 11.84RR376 pKa = 11.84SISDD380 pKa = 3.86WISGVVEE387 pKa = 4.55KK388 pKa = 10.89LFEE391 pKa = 4.93PIKK394 pKa = 10.81YY395 pKa = 9.61LVQEE399 pKa = 4.15ILDD402 pKa = 4.74LIRR405 pKa = 11.84PILVEE410 pKa = 3.55IAGEE414 pKa = 4.06IFKK417 pKa = 10.98LILDD421 pKa = 3.73ILFDD425 pKa = 4.13LVGILDD431 pKa = 3.98SLLKK435 pKa = 10.68KK436 pKa = 10.91LEE438 pKa = 4.16DD439 pKa = 3.76QIHH442 pKa = 6.08NLVDD446 pKa = 5.1LIRR449 pKa = 11.84VLLHH453 pKa = 6.27HH454 pKa = 7.13LFSIVTRR461 pKa = 11.84LLLSLEE467 pKa = 4.21CSYY470 pKa = 11.59HH471 pKa = 7.51LFEE474 pKa = 6.38ALFLVAFFRR483 pKa = 11.84YY484 pKa = 8.67IFHH487 pKa = 7.24SIYY490 pKa = 10.11IASLLTFFIFLIIGFEE506 pKa = 4.25RR507 pKa = 11.84IYY509 pKa = 10.11PSPVYY514 pKa = 10.48YY515 pKa = 10.58YY516 pKa = 10.74LSEE519 pKa = 4.12KK520 pKa = 10.3FRR522 pKa = 11.84LSVCSVPVRR531 pKa = 11.84YY532 pKa = 10.12LMNLQGAYY540 pKa = 10.05AVLKK544 pKa = 10.78DD545 pKa = 3.59LFEE548 pKa = 4.36YY549 pKa = 10.95LSGFMPNFLYY559 pKa = 10.48QGSYY563 pKa = 11.28DD564 pKa = 3.83LFIIASLMVSAFIISFFFSDD584 pKa = 3.34VV585 pKa = 3.09

Molecular weight: 67.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK08|A0A1L3KK08_9VIRU Uncharacterized protein OS=Hubei virga-like virus 15 OX=1923330 PE=4 SV=1
MM1 pKa = 7.53SSADD5 pKa = 3.73RR6 pKa = 11.84DD7 pKa = 3.83DD8 pKa = 3.8ARR10 pKa = 11.84TRR12 pKa = 11.84MVDD15 pKa = 2.83SRR17 pKa = 11.84GITTIKK23 pKa = 10.45GGNLTGATLIQSVQDD38 pKa = 3.27AYY40 pKa = 10.29TNVMQNAIATSMMALLVIITLAEE63 pKa = 3.97HH64 pKa = 6.57AQTKK68 pKa = 10.44GPLEE72 pKa = 4.27SMRR75 pKa = 11.84DD76 pKa = 3.4ALAALVDD83 pKa = 3.91SGEE86 pKa = 4.02MSGAKK91 pKa = 10.09NFIASAVHH99 pKa = 6.4NILVYY104 pKa = 10.2VVNHH108 pKa = 6.77KK109 pKa = 10.77DD110 pKa = 3.63VIIEE114 pKa = 4.26VAWIWWPWVVKK125 pKa = 10.34PSTKK129 pKa = 10.5NMWMSVVLSFFAVFAEE145 pKa = 4.14LTLFEE150 pKa = 4.65IVALANAYY158 pKa = 8.01FLWVSLRR165 pKa = 11.84NPAHH169 pKa = 6.31KK170 pKa = 9.07TLAFIFGMVILVVSLEE186 pKa = 4.03FNLTKK191 pKa = 10.36EE192 pKa = 4.31KK193 pKa = 10.53PLTPTDD199 pKa = 3.44IPHH202 pKa = 6.56TFDD205 pKa = 4.25PFPNVKK211 pKa = 10.36NPDD214 pKa = 3.99DD215 pKa = 4.72RR216 pKa = 11.84PPAPDD221 pKa = 3.35FATITKK227 pKa = 10.26KK228 pKa = 10.49EE229 pKa = 4.01GARR232 pKa = 11.84VARR235 pKa = 11.84SIGSGTIPPPTKK247 pKa = 9.99PFKK250 pKa = 10.87

Molecular weight: 27.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3289

250

2454

1096.3

125.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.142 ± 0.855

2.128 ± 0.391

6.811 ± 0.377

5.594 ± 0.727

5.655 ± 0.751

5.838 ± 0.39

2.402 ± 0.443

6.233 ± 0.241

5.564 ± 0.394

9.03 ± 0.889

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.01 ± 0.365

4.165 ± 0.211

3.922 ± 0.635

2.067 ± 0.173

6.476 ± 0.804

5.929 ± 0.087

5.442 ± 0.47

7.662 ± 0.315

0.973 ± 0.275

4.926 ± 0.685
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski