Clostridiaceae bacterium JG1575

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; unclassified Clostridiaceae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0N2U5|A0A2I0N2U5_9CLOT Methionine aminopeptidase OS=Clostridiaceae bacterium JG1575 OX=1658742 GN=map PE=3 SV=1
MM1 pKa = 8.25SEE3 pKa = 3.96EE4 pKa = 4.08KK5 pKa = 10.77KK6 pKa = 9.49FTEE9 pKa = 3.89EE10 pKa = 3.53AKK12 pKa = 10.89AEE14 pKa = 4.09EE15 pKa = 4.87SCCSHH20 pKa = 6.78EE21 pKa = 4.45EE22 pKa = 3.71AHH24 pKa = 7.3KK25 pKa = 10.4DD26 pKa = 3.53HH27 pKa = 7.16CCGEE31 pKa = 4.5HH32 pKa = 5.7KK33 pKa = 10.24HH34 pKa = 5.53EE35 pKa = 5.93HH36 pKa = 6.81DD37 pKa = 4.28GEE39 pKa = 4.56CCGEE43 pKa = 4.26HH44 pKa = 5.63KK45 pKa = 10.3HH46 pKa = 5.25EE47 pKa = 5.87HH48 pKa = 6.21NGEE51 pKa = 3.92CCKK54 pKa = 10.68NKK56 pKa = 10.36KK57 pKa = 8.36EE58 pKa = 4.08AEE60 pKa = 4.05EE61 pKa = 3.94AAEE64 pKa = 4.33EE65 pKa = 4.27VTLDD69 pKa = 3.85EE70 pKa = 5.49DD71 pKa = 4.11LEE73 pKa = 4.44EE74 pKa = 6.41DD75 pKa = 5.18GEE77 pKa = 4.47EE78 pKa = 4.09EE79 pKa = 3.79DD80 pKa = 3.83HH81 pKa = 6.58GAVVVMEE88 pKa = 4.68NEE90 pKa = 4.26DD91 pKa = 3.8GTTEE95 pKa = 4.15EE96 pKa = 4.78YY97 pKa = 10.44PVVDD101 pKa = 3.66EE102 pKa = 4.88FEE104 pKa = 4.63YY105 pKa = 11.15DD106 pKa = 3.11GSVYY110 pKa = 10.95VLVEE114 pKa = 4.09NADD117 pKa = 3.7GTVTPLRR124 pKa = 11.84ADD126 pKa = 3.74GEE128 pKa = 4.37DD129 pKa = 3.74GEE131 pKa = 5.08LAFLSEE137 pKa = 4.4EE138 pKa = 4.06EE139 pKa = 4.01FAEE142 pKa = 4.33VSNAYY147 pKa = 10.49NEE149 pKa = 4.61FEE151 pKa = 4.56DD152 pKa = 5.31AYY154 pKa = 11.27SDD156 pKa = 3.77EE157 pKa = 5.79DD158 pKa = 4.14DD159 pKa = 3.89MEE161 pKa = 4.59LVEE164 pKa = 5.28EE165 pKa = 5.25DD166 pKa = 3.29IEE168 pKa = 4.78IDD170 pKa = 3.55IEE172 pKa = 4.31EE173 pKa = 4.39KK174 pKa = 10.94

Molecular weight: 19.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0N1K5|A0A2I0N1K5_9CLOT Uncharacterized protein OS=Clostridiaceae bacterium JG1575 OX=1658742 GN=ABB02_00688 PE=4 SV=1
MM1 pKa = 7.32ARR3 pKa = 11.84EE4 pKa = 3.72ISPNKK9 pKa = 9.67EE10 pKa = 3.34NSTEE14 pKa = 4.07SRR16 pKa = 11.84SGGQRR21 pKa = 11.84EE22 pKa = 3.85EE23 pKa = 3.69RR24 pKa = 11.84RR25 pKa = 11.84EE26 pKa = 3.75YY27 pKa = 10.67RR28 pKa = 11.84RR29 pKa = 11.84TNPRR33 pKa = 11.84VRR35 pKa = 11.84RR36 pKa = 11.84SRR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 8.51VCQFCVDD47 pKa = 3.45HH48 pKa = 7.15VDD50 pKa = 3.81TIDD53 pKa = 3.6YY54 pKa = 10.86KK55 pKa = 11.14DD56 pKa = 3.51VPKK59 pKa = 10.4LRR61 pKa = 11.84KK62 pKa = 9.93YY63 pKa = 8.24ITEE66 pKa = 4.01RR67 pKa = 11.84GKK69 pKa = 10.04ILPRR73 pKa = 11.84RR74 pKa = 11.84ISGTCAKK81 pKa = 9.14HH82 pKa = 5.58QRR84 pKa = 11.84VLTEE88 pKa = 4.42AIKK91 pKa = 10.29RR92 pKa = 11.84ARR94 pKa = 11.84NIALLPFTNDD104 pKa = 2.6

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2035

0

2035

668580

30

2118

328.5

36.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.732 ± 0.046

1.014 ± 0.018

5.025 ± 0.038

7.129 ± 0.063

4.09 ± 0.036

7.434 ± 0.046

2.096 ± 0.024

5.796 ± 0.048

5.755 ± 0.049

11.202 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.024

3.267 ± 0.036

4.528 ± 0.039

3.806 ± 0.031

5.75 ± 0.047

5.714 ± 0.035

5.172 ± 0.041

6.744 ± 0.043

0.951 ± 0.019

3.094 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski