Varroa mite associated genomovirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; unclassified Genomoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I8B2L9|A0A2I8B2L9_9VIRU Capsid protein OS=Varroa mite associated genomovirus 1 OX=2077299 PE=4 SV=1
MM1 pKa = 7.77PSAFHH6 pKa = 6.55LKK8 pKa = 9.2NRR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 10.59VLFTYY17 pKa = 10.25SQAGPDD23 pKa = 3.36FDD25 pKa = 3.8YY26 pKa = 10.74WAVVDD31 pKa = 5.11LLGDD35 pKa = 4.08LGAEE39 pKa = 3.99CVIGRR44 pKa = 11.84EE45 pKa = 3.95EE46 pKa = 3.96HH47 pKa = 6.75ADD49 pKa = 3.49GGIHH53 pKa = 5.86FHH55 pKa = 6.37VFTDD59 pKa = 4.01FGRR62 pKa = 11.84LFSTRR67 pKa = 11.84KK68 pKa = 9.62VRR70 pKa = 11.84VFDD73 pKa = 4.04VGGKK77 pKa = 9.28HH78 pKa = 6.77PNIQPIGRR86 pKa = 11.84TPATAYY92 pKa = 10.46DD93 pKa = 3.92YY94 pKa = 10.93AIKK97 pKa = 10.92DD98 pKa = 3.52GDD100 pKa = 4.04VVAGGLGRR108 pKa = 11.84PGGDD112 pKa = 4.46CDD114 pKa = 3.84WDD116 pKa = 3.81PDD118 pKa = 4.21NFWSSAAHH126 pKa = 6.35CGSSDD131 pKa = 3.73EE132 pKa = 4.63FLHH135 pKa = 7.2FCDD138 pKa = 4.74QLATRR143 pKa = 11.84DD144 pKa = 4.14LIKK147 pKa = 10.71SFPNFRR153 pKa = 11.84AYY155 pKa = 10.47ANWKK159 pKa = 7.95WNSGVPEE166 pKa = 3.98YY167 pKa = 10.39DD168 pKa = 3.22QPAGAIFDD176 pKa = 3.85TSAAPGIDD184 pKa = 3.0EE185 pKa = 4.61WLSQSALGTGKK196 pKa = 10.2SRR198 pKa = 11.84ARR200 pKa = 3.48

Molecular weight: 22.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I8B2L9|A0A2I8B2L9_9VIRU Capsid protein OS=Varroa mite associated genomovirus 1 OX=2077299 PE=4 SV=1
MM1 pKa = 7.07SSKK4 pKa = 10.7RR5 pKa = 11.84ILNLTSTKK13 pKa = 10.4KK14 pKa = 10.23KK15 pKa = 10.86DD16 pKa = 3.66VMLQYY21 pKa = 11.06TNIDD25 pKa = 3.73FGSISPGAYY34 pKa = 8.41NQKK37 pKa = 9.06EE38 pKa = 3.75AAMTGNFRR46 pKa = 11.84YY47 pKa = 8.31MIPWIATGRR56 pKa = 11.84PGIDD60 pKa = 2.84ISGGGAGPIDD70 pKa = 3.74EE71 pKa = 4.48STRR74 pKa = 11.84TKK76 pKa = 7.5TTCFMRR82 pKa = 11.84GLKK85 pKa = 10.18EE86 pKa = 4.45SITLRR91 pKa = 11.84SGDD94 pKa = 3.67GQPWLWRR101 pKa = 11.84RR102 pKa = 11.84ICFTLKK108 pKa = 9.95GDD110 pKa = 5.02RR111 pKa = 11.84IHH113 pKa = 6.77SLQAAGMNLYY123 pKa = 10.29DD124 pKa = 3.87RR125 pKa = 11.84QNGAGASGVIRR136 pKa = 11.84PATNWPATTPGGAEE150 pKa = 4.06LEE152 pKa = 4.07QLMFRR157 pKa = 11.84GVRR160 pKa = 11.84NIDD163 pKa = 3.14WADD166 pKa = 3.36SFLAPLDD173 pKa = 3.48STRR176 pKa = 11.84ISVKK180 pKa = 9.94YY181 pKa = 10.78DD182 pKa = 2.85KK183 pKa = 8.59TTTLASGNSEE193 pKa = 3.93GFIRR197 pKa = 11.84TYY199 pKa = 10.82HH200 pKa = 6.44RR201 pKa = 11.84WHH203 pKa = 7.17PMNKK207 pKa = 9.09NLVYY211 pKa = 10.83GDD213 pKa = 4.2DD214 pKa = 3.69QNGQSVQLTPWSTEE228 pKa = 3.64GKK230 pKa = 9.96QGMGDD235 pKa = 4.0FYY237 pKa = 11.72VVDD240 pKa = 4.38LFKK243 pKa = 11.61SNGNPDD249 pKa = 3.22AQLGLRR255 pKa = 11.84YY256 pKa = 9.73NATLYY261 pKa = 9.25WHH263 pKa = 7.02EE264 pKa = 4.27KK265 pKa = 9.3

Molecular weight: 29.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

799

200

334

266.3

29.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.26 ± 0.787

1.502 ± 0.317

8.26 ± 0.939

3.504 ± 0.361

5.507 ± 0.893

10.638 ± 0.24

3.004 ± 0.67

4.631 ± 0.439

4.255 ± 0.435

7.259 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.627 ± 0.752

3.63 ± 0.915

4.881 ± 0.19

3.004 ± 0.502

6.508 ± 0.2

6.508 ± 0.327

5.382 ± 1.269

4.631 ± 0.693

3.63 ± 0.453

3.379 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski