Geobacter sp. Red88

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3510 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A9XFR3|A0A5A9XFR3_9DELT NCS2 family permease OS=Geobacter sp. Red88 OX=2509454 GN=ET418_07535 PE=3 SV=1
MM1 pKa = 7.32EE2 pKa = 5.12RR3 pKa = 11.84WICTICQYY11 pKa = 11.28VYY13 pKa = 11.06DD14 pKa = 4.95PATGDD19 pKa = 3.62PDD21 pKa = 4.01RR22 pKa = 11.84GVPPGTPFEE31 pKa = 4.48SLPDD35 pKa = 3.51DD36 pKa = 3.85WTCPLCGAGKK46 pKa = 10.06DD47 pKa = 3.71AFEE50 pKa = 5.15KK51 pKa = 10.55EE52 pKa = 4.16

Molecular weight: 5.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A9XDL5|A0A5A9XDL5_9DELT MerR family transcriptional regulator OS=Geobacter sp. Red88 OX=2509454 GN=ET418_12355 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNTSRR12 pKa = 11.84KK13 pKa = 7.49RR14 pKa = 11.84THH16 pKa = 6.16GFLVRR21 pKa = 11.84MSTKK25 pKa = 10.31NGRR28 pKa = 11.84LVIKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.27SLSVRR45 pKa = 11.84IAAKK49 pKa = 10.27

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3510

0

3510

1106654

28

3694

315.3

34.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.996 ± 0.049

1.315 ± 0.019

5.176 ± 0.028

6.261 ± 0.047

3.969 ± 0.026

8.06 ± 0.037

2.11 ± 0.023

6.037 ± 0.032

4.783 ± 0.047

10.152 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.018

3.293 ± 0.033

4.615 ± 0.03

3.257 ± 0.023

6.2 ± 0.049

5.705 ± 0.042

5.496 ± 0.049

7.245 ± 0.033

1.0 ± 0.015

2.752 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski