Butyrivibrio proteoclasticus (strain ATCC 51982 / DSM 14932 / B316) (Clostridium proteoclasticum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; Butyrivibrio proteoclasticus

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3799 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0RZY4|E0RZY4_BUTPB Acyltransferase OS=Butyrivibrio proteoclasticus (strain ATCC 51982 / DSM 14932 / B316) OX=515622 GN=bpr_I0437 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.42KK3 pKa = 10.14KK4 pKa = 10.78LSVLLAGVMVCTMFTACGKK23 pKa = 10.25EE24 pKa = 4.05NASEE28 pKa = 4.08NADD31 pKa = 3.31NAASTEE37 pKa = 3.87NSADD41 pKa = 3.33AAATEE46 pKa = 4.27TSVAEE51 pKa = 4.59GMALTIDD58 pKa = 4.19FDD60 pKa = 4.1NLEE63 pKa = 4.43TEE65 pKa = 4.34TLNDD69 pKa = 3.24VTASDD74 pKa = 3.86IVTLGQYY81 pKa = 10.14KK82 pKa = 9.97EE83 pKa = 4.35LSFEE87 pKa = 4.14VAKK90 pKa = 11.18DD91 pKa = 3.57EE92 pKa = 4.36VTDD95 pKa = 4.67AEE97 pKa = 4.45VEE99 pKa = 4.35DD100 pKa = 4.44YY101 pKa = 10.91VANLWSTKK109 pKa = 10.35PLMLDD114 pKa = 3.2VTDD117 pKa = 5.0RR118 pKa = 11.84AVQDD122 pKa = 3.49GDD124 pKa = 4.11TVNIDD129 pKa = 3.63YY130 pKa = 10.54VGKK133 pKa = 10.53YY134 pKa = 10.47ADD136 pKa = 3.68TKK138 pKa = 10.45EE139 pKa = 4.28AFDD142 pKa = 3.99GGTAEE147 pKa = 4.6GASLTIGSNSYY158 pKa = 10.55IEE160 pKa = 4.6GFEE163 pKa = 4.17SGLVGVNIGDD173 pKa = 3.92TVDD176 pKa = 4.04LNLTFPEE183 pKa = 4.25NYY185 pKa = 9.72GAEE188 pKa = 4.06NLAGKK193 pKa = 10.07DD194 pKa = 3.3VVFTVTVNSIQAPDD208 pKa = 3.6TEE210 pKa = 5.27MSDD213 pKa = 2.92EE214 pKa = 3.93WAAGLGYY221 pKa = 10.82DD222 pKa = 4.84GVTDD226 pKa = 4.6LATLRR231 pKa = 11.84DD232 pKa = 4.0YY233 pKa = 11.38ARR235 pKa = 11.84KK236 pKa = 7.73TLEE239 pKa = 4.24DD240 pKa = 3.27QAQNEE245 pKa = 4.24FDD247 pKa = 3.7STVEE251 pKa = 3.8NTAVQTVYY259 pKa = 11.22DD260 pKa = 3.85STAFGDD266 pKa = 3.83IPQALINRR274 pKa = 11.84YY275 pKa = 7.78MKK277 pKa = 9.8QQKK280 pKa = 9.89QMLDD284 pKa = 3.66YY285 pKa = 10.4QATMYY290 pKa = 10.38SYY292 pKa = 11.61YY293 pKa = 10.63YY294 pKa = 9.95GQQLTADD301 pKa = 4.0SLLANYY307 pKa = 8.23MNAEE311 pKa = 4.15GFVGTVDD318 pKa = 5.59DD319 pKa = 4.35YY320 pKa = 12.15LNDD323 pKa = 3.31ISKK326 pKa = 11.13DD327 pKa = 3.29MAQQYY332 pKa = 11.55LMFQAIADD340 pKa = 4.06EE341 pKa = 4.39EE342 pKa = 4.67NITVSDD348 pKa = 4.12EE349 pKa = 4.51EE350 pKa = 3.75IDD352 pKa = 4.53AYY354 pKa = 11.15LKK356 pKa = 10.55NAYY359 pKa = 8.9EE360 pKa = 4.23NASTTSFSSFEE371 pKa = 4.14EE372 pKa = 4.31YY373 pKa = 10.41KK374 pKa = 10.82EE375 pKa = 4.16SLDD378 pKa = 3.92LEE380 pKa = 4.66IYY382 pKa = 10.59RR383 pKa = 11.84EE384 pKa = 3.83GLMADD389 pKa = 3.38KK390 pKa = 10.85VVAFIVEE397 pKa = 4.17NANVVATAATEE408 pKa = 4.09DD409 pKa = 3.75AASEE413 pKa = 4.28SSSAEE418 pKa = 3.63ASADD422 pKa = 3.44AASEE426 pKa = 4.24SSSTEE431 pKa = 3.88TVAEE435 pKa = 4.14

Molecular weight: 47.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0RW10|E0RW10_BUTPB AAA-ATPase_like domain-containing protein OS=Butyrivibrio proteoclasticus (strain ATCC 51982 / DSM 14932 / B316) OX=515622 GN=bpr_I0125 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 8.74MTFQPHH8 pKa = 5.93KK9 pKa = 8.68LQRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3799

0

3799

1301855

30

5853

342.7

38.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.2 ± 0.046

1.373 ± 0.017

6.63 ± 0.033

7.243 ± 0.042

4.317 ± 0.03

6.819 ± 0.039

1.635 ± 0.015

7.696 ± 0.041

7.02 ± 0.036

8.329 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.993 ± 0.02

4.905 ± 0.03

3.079 ± 0.022

2.76 ± 0.019

4.064 ± 0.029

6.279 ± 0.032

5.345 ± 0.039

6.777 ± 0.028

0.938 ± 0.016

4.597 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski