Bacillus phage 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Svunavirus; Bacillus virus 1

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6XMI7|A6XMI7_9CAUD Uncharacterized protein OS=Bacillus phage 1 OX=2785079 PE=4 SV=1
MM1 pKa = 7.59LVRR4 pKa = 11.84FSLLYY9 pKa = 9.85KK10 pKa = 10.2SGYY13 pKa = 9.27EE14 pKa = 3.8DD15 pKa = 4.64VIEE18 pKa = 4.23QVCLPSEE25 pKa = 4.24VGNIIQTIRR34 pKa = 11.84GSFYY38 pKa = 10.24EE39 pKa = 4.68DD40 pKa = 3.15SPGYY44 pKa = 10.27ISFGDD49 pKa = 3.98EE50 pKa = 3.8NLKK53 pKa = 10.86GYY55 pKa = 9.82IINVQEE61 pKa = 4.09VARR64 pKa = 11.84VKK66 pKa = 10.39MEE68 pKa = 3.72ILEE71 pKa = 4.77GGDD74 pKa = 3.43SS75 pKa = 3.4

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3RH36|B3RH36_9CAUD FtsK/SpoiiiE family protein OS=Bacillus phage 1 OX=2785079 PE=4 SV=1
MM1 pKa = 7.42FGLGKK6 pKa = 9.64PRR8 pKa = 11.84SKK10 pKa = 10.37FGKK13 pKa = 8.39WVDD16 pKa = 3.65RR17 pKa = 11.84MGLTQEE23 pKa = 4.24EE24 pKa = 4.75IARR27 pKa = 11.84KK28 pKa = 9.44AKK30 pKa = 10.2VGRR33 pKa = 11.84TTISNMCKK41 pKa = 10.17DD42 pKa = 3.79PEE44 pKa = 4.24YY45 pKa = 10.71KK46 pKa = 10.08PRR48 pKa = 11.84ISTWVKK54 pKa = 9.66VEE56 pKa = 4.07RR57 pKa = 11.84ALKK60 pKa = 10.07SLGYY64 pKa = 8.81SVKK67 pKa = 10.63RR68 pKa = 11.84EE69 pKa = 3.94DD70 pKa = 4.72FFDD73 pKa = 3.24II74 pKa = 4.78

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10591

32

955

196.1

22.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.308 ± 0.564

0.935 ± 0.171

5.675 ± 0.246

8.148 ± 0.374

4.287 ± 0.261

6.052 ± 0.331

1.813 ± 0.218

7.261 ± 0.276

7.875 ± 0.366

8.649 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.169

4.579 ± 0.268

3.305 ± 0.185

3.73 ± 0.218

5.731 ± 0.316

5.193 ± 0.301

5.278 ± 0.294

6.307 ± 0.31

1.19 ± 0.132

4.041 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski