gamma proteobacterium IMCC1989

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; unclassified Alteromonadales

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3LI17|F3LI17_9GAMM Uncharacterized protein OS=gamma proteobacterium IMCC1989 OX=937772 GN=IMCC1989_1176 PE=4 SV=1
MM1 pKa = 7.85SDD3 pKa = 2.97NSYY6 pKa = 9.12DD7 pKa = 3.37TTTDD11 pKa = 3.77LNPSSSCLGFDD22 pKa = 4.55DD23 pKa = 5.0FCNLLMPYY31 pKa = 10.26GALNSPAEE39 pKa = 4.09LHH41 pKa = 6.78GLLCGKK47 pKa = 10.04LCGGAKK53 pKa = 9.03LTRR56 pKa = 11.84DD57 pKa = 3.86DD58 pKa = 3.62WLQAAWEE65 pKa = 4.25FLDD68 pKa = 5.36LTDD71 pKa = 4.55TPDD74 pKa = 3.19GQAQDD79 pKa = 3.99EE80 pKa = 4.71VAMLLDD86 pKa = 3.34VTMQQLTSGMYY97 pKa = 10.73DD98 pKa = 3.26MQLLLPEE105 pKa = 5.04NDD107 pKa = 3.13TDD109 pKa = 4.87LDD111 pKa = 3.64QRR113 pKa = 11.84TQALAEE119 pKa = 4.06WCHH122 pKa = 5.68GFLGGFGTAGIDD134 pKa = 3.31PDD136 pKa = 3.72KK137 pKa = 11.12TFTSDD142 pKa = 3.32AADD145 pKa = 3.83ALRR148 pKa = 11.84DD149 pKa = 3.42MAAIVQAAVSDD160 pKa = 4.11EE161 pKa = 4.04EE162 pKa = 5.38DD163 pKa = 3.53EE164 pKa = 4.43EE165 pKa = 4.36EE166 pKa = 4.52QEE168 pKa = 4.63KK169 pKa = 10.91DD170 pKa = 3.44YY171 pKa = 11.61SDD173 pKa = 4.72LIEE176 pKa = 4.33YY177 pKa = 10.42VRR179 pKa = 11.84MAAMHH184 pKa = 6.88FYY186 pKa = 10.88AEE188 pKa = 4.03HH189 pKa = 7.11HH190 pKa = 6.78INEE193 pKa = 5.0DD194 pKa = 3.67DD195 pKa = 4.39LPTVHH200 pKa = 7.11

Molecular weight: 22.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3LIR4|F3LIR4_9GAMM Transcriptional regulator OS=gamma proteobacterium IMCC1989 OX=937772 GN=IMCC1989_1555 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84GPHH5 pKa = 5.46NLKK8 pKa = 8.85HH9 pKa = 5.16TFGRR13 pKa = 11.84RR14 pKa = 11.84LRR16 pKa = 11.84AVGVPYY22 pKa = 7.93EE23 pKa = 4.18TRR25 pKa = 11.84QVLLGHH31 pKa = 6.82RR32 pKa = 11.84NGDD35 pKa = 3.6VTTHH39 pKa = 5.61YY40 pKa = 10.83SGAEE44 pKa = 3.84LQEE47 pKa = 5.01LIDD50 pKa = 3.95AAEE53 pKa = 4.21KK54 pKa = 10.55VVSEE58 pKa = 4.22KK59 pKa = 10.88SRR61 pKa = 11.84AYY63 pKa = 10.2AYY65 pKa = 8.49KK66 pKa = 10.46KK67 pKa = 9.71PALRR71 pKa = 11.84LLRR74 pKa = 11.84RR75 pKa = 11.84AVNRR79 pKa = 3.82

Molecular weight: 9.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2844

0

2844

816074

37

2598

286.9

31.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.387 ± 0.046

1.018 ± 0.017

5.798 ± 0.039

6.215 ± 0.048

3.926 ± 0.038

6.486 ± 0.042

2.311 ± 0.027

6.847 ± 0.036

5.492 ± 0.042

10.08 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.024

4.527 ± 0.04

3.8 ± 0.03

4.442 ± 0.034

4.527 ± 0.043

7.103 ± 0.047

5.558 ± 0.042

6.929 ± 0.043

1.122 ± 0.019

3.034 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski