Butyrivibrio crossotus CAG:259

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; environmental samples

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5LWY2|R5LWY2_9FIRM YitL protein OS=Butyrivibrio crossotus CAG:259 OX=1263062 GN=BN569_00806 PE=3 SV=1
MM1 pKa = 7.76KK2 pKa = 10.11EE3 pKa = 3.82PEE5 pKa = 3.74IDD7 pKa = 3.48IEE9 pKa = 4.18EE10 pKa = 4.74DD11 pKa = 3.34STDD14 pKa = 3.36IEE16 pKa = 4.53VIEE19 pKa = 4.83IDD21 pKa = 3.91SDD23 pKa = 4.27TIDD26 pKa = 3.62GAAADD31 pKa = 4.42SDD33 pKa = 4.16VASNIQNVSEE43 pKa = 4.34EE44 pKa = 4.2NDD46 pKa = 3.68PPVQPDD52 pKa = 3.51NEE54 pKa = 4.27IEE56 pKa = 4.23NTDD59 pKa = 3.34TTEE62 pKa = 3.86QTTSDD67 pKa = 4.39DD68 pKa = 3.71DD69 pKa = 4.38FEE71 pKa = 6.31IIPAEE76 pKa = 4.25AVPDD80 pKa = 4.73DD81 pKa = 4.12YY82 pKa = 11.88DD83 pKa = 4.51NYY85 pKa = 10.66ILTPDD90 pKa = 3.13NRR92 pKa = 11.84KK93 pKa = 8.66FHH95 pKa = 5.81ITKK98 pKa = 9.97FDD100 pKa = 2.95IRR102 pKa = 11.84RR103 pKa = 11.84YY104 pKa = 9.64IIIFILTCVMVFCMYY119 pKa = 10.38QIISRR124 pKa = 11.84YY125 pKa = 8.76IGYY128 pKa = 10.09ARR130 pKa = 11.84EE131 pKa = 3.8RR132 pKa = 11.84RR133 pKa = 11.84HH134 pKa = 5.89NSEE137 pKa = 3.85INNSVIEE144 pKa = 4.59EE145 pKa = 4.19NTSTPDD151 pKa = 4.25LSTDD155 pKa = 3.2TYY157 pKa = 11.77KK158 pKa = 11.13NINPGLAIDD167 pKa = 4.48FDD169 pKa = 4.45KK170 pKa = 11.16LLQINEE176 pKa = 4.24ACAGWIQIPSVGISYY191 pKa = 8.87PIVQGPDD198 pKa = 2.56NDD200 pKa = 4.23YY201 pKa = 10.75YY202 pKa = 11.43LHH204 pKa = 6.9YY205 pKa = 10.64DD206 pKa = 3.49ISQNEE211 pKa = 3.99AWSGAIYY218 pKa = 10.54LDD220 pKa = 3.65YY221 pKa = 11.04RR222 pKa = 11.84SDD224 pKa = 3.46RR225 pKa = 11.84SLDD228 pKa = 3.58EE229 pKa = 3.76QHH231 pKa = 5.27FTIYY235 pKa = 9.58GHH237 pKa = 6.48HH238 pKa = 6.56MNDD241 pKa = 2.74GSMFANLLKK250 pKa = 10.66YY251 pKa = 10.38DD252 pKa = 4.39DD253 pKa = 4.35PEE255 pKa = 4.65FYY257 pKa = 10.54QKK259 pKa = 10.79NQDD262 pKa = 3.14NFNNYY267 pKa = 8.74IYY269 pKa = 9.74IYY271 pKa = 9.8QKK273 pKa = 10.89GRR275 pKa = 11.84VLVYY279 pKa = 9.98QIFSVVDD286 pKa = 3.39TYY288 pKa = 11.83YY289 pKa = 11.25DD290 pKa = 3.5SDD292 pKa = 4.11PEE294 pKa = 4.26SFLVLISDD302 pKa = 4.55DD303 pKa = 3.97EE304 pKa = 4.67TKK306 pKa = 10.63KK307 pKa = 10.95SYY309 pKa = 10.95LNHH312 pKa = 6.22IRR314 pKa = 11.84QKK316 pKa = 10.32QLYY319 pKa = 5.64EE320 pKa = 3.69TGYY323 pKa = 10.6FADD326 pKa = 3.82TNDD329 pKa = 4.26RR330 pKa = 11.84LLTLYY335 pKa = 9.21TCQSDD340 pKa = 3.86STSKK344 pKa = 9.78EE345 pKa = 3.33RR346 pKa = 11.84HH347 pKa = 4.69MVHH350 pKa = 6.77AKK352 pKa = 10.41LIAEE356 pKa = 4.63ISNN359 pKa = 3.56

Molecular weight: 41.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5LUI2|R5LUI2_9FIRM Oxidoreductase Gfo/Idh/MocA family OS=Butyrivibrio crossotus CAG:259 OX=1263062 GN=BN569_00355 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.27VHH16 pKa = 6.08GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 4.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1976

0

1976

629432

30

1985

318.5

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.744 ± 0.058

1.41 ± 0.022

6.326 ± 0.045

7.229 ± 0.055

4.154 ± 0.044

6.837 ± 0.056

1.479 ± 0.023

8.739 ± 0.055

7.849 ± 0.047

8.072 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.003 ± 0.025

5.419 ± 0.04

2.871 ± 0.028

2.165 ± 0.024

3.925 ± 0.035

6.011 ± 0.044

5.533 ± 0.048

7.084 ± 0.045

0.644 ± 0.018

4.501 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski