Nocardioides simplex (Arthrobacter simplex)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Pimelobacter

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5426 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1DLI9|A0A0A1DLI9_NOCSI Uncharacterized protein OS=Nocardioides simplex OX=2045 GN=KR76_04770 PE=4 SV=1
MM1 pKa = 8.12SLMTAASACSSAPDD15 pKa = 3.19SDD17 pKa = 3.51APTRR21 pKa = 11.84FTIRR25 pKa = 11.84PDD27 pKa = 3.87DD28 pKa = 3.56YY29 pKa = 10.45HH30 pKa = 8.37VPYY33 pKa = 10.57AGTAADD39 pKa = 3.45GRR41 pKa = 11.84RR42 pKa = 11.84FFLSEE47 pKa = 3.91EE48 pKa = 4.32LGTPEE53 pKa = 6.24DD54 pKa = 4.13PDD56 pKa = 3.1TWYY59 pKa = 11.14VGLFLWHH66 pKa = 7.5PDD68 pKa = 2.97GSFDD72 pKa = 3.64EE73 pKa = 5.1VVAEE77 pKa = 4.59RR78 pKa = 11.84VATAPASDD86 pKa = 4.0RR87 pKa = 11.84LRR89 pKa = 11.84EE90 pKa = 4.04TYY92 pKa = 9.99LASLGDD98 pKa = 3.93YY99 pKa = 9.95VLEE102 pKa = 4.35PVEE105 pKa = 4.18VTPFTTEE112 pKa = 2.93IDD114 pKa = 4.14GITFGWAVSEE124 pKa = 4.21YY125 pKa = 10.53QGIWSINIEE134 pKa = 4.28PGDD137 pKa = 3.9FIAYY141 pKa = 9.39NEE143 pKa = 4.1PWDD146 pKa = 3.98GVDD149 pKa = 3.55YY150 pKa = 8.86DD151 pKa = 3.95TT152 pKa = 5.87

Molecular weight: 16.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5XB63|A0A0C5XB63_NOCSI Indolepyruvate oxidoreductase subunit IorB II OS=Nocardioides simplex OX=2045 GN=KR76_15960 PE=4 SV=1
MM1 pKa = 7.44ARR3 pKa = 11.84SSLLRR8 pKa = 11.84PTVAAAPGPRR18 pKa = 11.84EE19 pKa = 4.01GRR21 pKa = 11.84RR22 pKa = 11.84SRR24 pKa = 11.84AGRR27 pKa = 11.84RR28 pKa = 11.84AVPTRR33 pKa = 11.84SGRR36 pKa = 11.84SRR38 pKa = 11.84LPWGRR43 pKa = 3.46

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5426

0

5426

1722844

37

2714

317.5

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.869 ± 0.049

0.728 ± 0.009

6.507 ± 0.031

5.377 ± 0.029

2.661 ± 0.021

9.442 ± 0.034

2.116 ± 0.015

3.397 ± 0.021

1.953 ± 0.027

10.326 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.668 ± 0.014

1.717 ± 0.018

5.829 ± 0.027

2.651 ± 0.016

7.624 ± 0.038

4.915 ± 0.024

6.227 ± 0.039

9.509 ± 0.031

1.507 ± 0.014

1.976 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski